Table of Content

25 November 2018, Volume 38 Issue 6

Previous Issue    Next Issue

Modified nonmonotone memory gradient method for unconstrained optimization

SU Ke, REN Lele, RONG Zixing, XU Chun

2018, 38(6):  561-566.  DOI: 10.3969/j.issn.1000-1565.2018.06.001

Asbtract ( 439 )   PDF (926KB) ( 248 )  

References | Related Articles | Metrics

A modified nonmonotone line search memory gradient method for unconstrained optimization is proposed in this paper. In this method, a new criterion is constructed to decide whether the trial point is acceptable or not. The presented algorithm is a generalization of the existing nonmonotone-type methods. Under some reasonable conditions, the global convergent properties are proved. The numerical results show that the algorithm is effective and flexible.

A class of close-to-convex analytic functions

QIAO Jinjing, HUANG Miaomiao, GUO Qiannan

2018, 38(6):  567-571.  DOI: 10.3969/j.issn.1000-1565.2018.06.002

Asbtract ( 468 )   PDF (901KB) ( 203 )  

References | Related Articles | Metrics

A class of close-to-convex functions is investigated, which is the generalization of the class of analytic functions whose derivatives have positive real parts. The following properties are discussed: distortion theorems, the radius of convexity, close-to-convexity of the partial sums and extremal functions.The subclass which contains functions with initial zero coefficients is also studied.

Mechanical properties of reinforced concrete columns under high temperature and axial load

NIU Xiaoyan, SHEN Linlin, CHEN Cong, DU Erxia

2018, 38(6):  572-575.  DOI: 10.3969/j.issn.1000-1565.2018.06.003

Asbtract ( 492 )   PDF (1819KB) ( 289 )  

References | Related Articles | Metrics

For the destruction of concrete columns at high temperature, the mechanical behavior of axially loaded reinforced concrete columns was studied based on the finite element analysis software ANSYS at different temperatures. The results show that the reinforced concrete columns show a downward deformation from top to bottom at all ambient temperatures. Meanwhile, the column shows a more obvious temperature effect. The overall distribution of equivalent stress is almost uniform in the model. Yet, there is a significant increase from the inside constraint surface to the outside constraint surface, and the equivalent stress reaches the maximum value at the boundary of the constraint surface.

Effect of film thickness of carbon fiber supported Re-Pt₃Ni composite on the performance of dye sensitized solar cells

YU Minsi, XIE Jianjun, SHAN Yiyang, ZHAO Hengqi, LAI Weidong, XIAO Jinchong, LI Ling

2018, 38(6):  576-581.  DOI: 10.3969/j.issn.1000-1565.2018.06.004

Asbtract ( 477 )   PDF (1661KB) ( 180 )  

References | Related Articles | Metrics

Carbon nanofibers were prepared by electrospinning, then Re-Pt₃Ni Supported by Carbon Nanofibers Composite were prepared by heating in oil bath. The composite electrodes with different film thickness were prepared by ball milling and spraying, and the electrodes were 5, 10, 15 and 20 μm respectively. The influence of film thickness on the performance of dye-sensitized solar cells was studied, Energy conversion efficiency were 5.12%, 7.67%, 8.43%, 8.09%. The results show that with the increase of the thickness of the electrode film, the performance of DSSCs first increases and then decreases, and when the thickness of the electrode is about 15 μm, the performance of DSSCs is the best. At the same time, it also- DOI:10.3969/j.issn.1000-1565.2018.06.004碳纤维负载Re-Pt₃Ni复合材料对电极膜厚对染敏电池性能影响于敏思¹,解建军¹,单一洋¹,赵恒启¹,赖伟东¹,肖金冲²,李玲¹(1.河北大学 物理科学与技术学院,河北省光电信息材料重点实验室,河北 保定 071002;2. 河北大学 化学与环境科学学院,河北 保定 071002)摘 要:采用静电纺丝技术制备碳纳米纤维,通过油浴加热法制备碳纳米纤维负载Re-Pt₃Ni复合材料.为了研究该复合材料对电极的膜厚对染料敏化太阳能电池性能的影响,通过球磨以及喷涂法得到不同膜厚的对电极,分别为5、10、15、20 μm,所得电池的能量转换效率分别为5.12%、7.67%、8.43%、8.09%.结果表明复合材料对电极膜厚存在一个最佳值,使电池的能量转换效率达到最高,同时,也表明复合材料有潜力成为Pt电极的替代品.关键词:染料敏化太阳能电池;对电极;碳纳米纤维;复合材料中图分类号:O643 文献标志码:A 【additional_page=587】文章编号:1000-1565(2018)06-0576-06Effect of film thickness of carbon fiber supported Re-Pt₃Ni composite on the performance of dye sensitized solar cellsYU Minsi¹, XIE Jianjun¹, SHAN Yiyang¹, ZHAO Hengqi¹, LAI Weidong¹, XIAO Jinchong², LI Ling¹(1.Hebei Key Lab of Optic-electronic Information and Materials, College of Physics Science and Technology, Hebei University, Baoding 071002, China;2. College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China)Abstract: Carbon nanofibers were prepared by electrospinning, then Re-Pt₃Ni Supported by Carbon Nanofibers Composite were prepared by heating in oil bath. The composite electrodes with different film thickness were prepared by ball milling and spraying, and the electrodes were 5, 10, 15 and 20 μm respectively. The influence of film thickness on the performance of dye-sensitized solar cells was studied, Energy conversion efficiency were 5.12%, 7.67%, 8.43%, 8.09%. The results show that with the increase of the thickness of the electrode film, the performance of DSSCs first increases and then decreases, and when the thickness of the electrode is about 15 μm, the performance of DSSCs is the best. At the same time, it also- 收稿日期:2018-06-26 基金项目:国家自然科学基金资助项目(51772073;21672051;21201053;51607054;51762013);河北大学研究生创新资助项目(hbu2018ss63);河北省杰出青年基金资助项目(A2017201082;A2018201019);河北省第二批青年拔尖人才计划项目(702800116025;70280011808);河北大学杰出青年基金资助项目(2015JQ02);河北大学2018年实验室开放项目(sy201849;sy201850) 第一作者:于敏思(1994—),女,北京平谷人,河北大学在读硕士研究生,主要从事新型纳米光电材料与器件研究.E-mail:minsiyuhbu@163.com 通信作者:李玲(1980—),女,河北保定人,河北大学副教授,主要从事新型纳米光电材料与器件研究.E-mail:lilinghbu@163.com第6期于敏思等:碳纤维负载Re-Pt₃Ni复合材料对电极膜厚对染敏电池性能影响shows that the composite has the potential to replace the Pt material.

Design of detection circuit system for MEMS optical fiber pressure sensor

DING Zhenjun,AN Jialin,JIA Kejun,WEI Zihui

2018, 38(6):  582-587.  DOI: 10.3969/j.issn.1000-1565.2018.06.005

Asbtract ( 596 )   PDF (1281KB) ( 474 )  

References | Related Articles | Metrics

According to the characteristics of reflective intensity modulated MEMS fiber optic pressure sensor, a detection circuit system based on STC89C52 microcontroller is designed. The system is divided into photoelectric conversion module, attenuation filter module, true RMS conversion module, A/D conversion module and liquid crystal display module. Using OPT101 as photoelectric conversion device, the light intensity signal transmitted by optical fiber is converted into voltage signal. After attenuation of voltage signal, it is output to AC/DC converter and A/D converter through filter. The results are displayed on the LCD screen and can be output to the computer through the interface. The test results show that the designed circuit has high precision and resolution.

Salt effects on the aggregation of reduced-denatured lysozyme

JIA Baohuan, YU Yang, WANG Yue, LIU Siyang, DU Baoan, MA Gang

2018, 38(6):  588-595.  DOI: 10.3969/j.issn.1000-1565.2018.06.006

Asbtract ( 424 )   PDF (2138KB) ( 224 )  

References | Related Articles | Metrics

Using reduced-denatured lysozyme as a model protein, the effects of sodium sulfate(Na₂SO₄), sodium perchlorate(NaClO₄), sodium nitrate(NaNO₃), and sodium chloride(NaCl)on protein aggregation were investigated. Turbidity assay were used to study the critical salt concentration on protein aggregation and the aggregation kinetics. It was found that the critical salt concentrations increased by following the order of SO₄^2- > ClO₄^- > NO₃^- > Cl^- and the aggregation rates decreased by following the order of SO₄^2- > ClO₄^- > NO₃^- > Cl^-.The fitted kinetic parameters showed a linear relationship with salt concentrations. All of the four salts induced the formation of amorphous protein aggregates as revealed by scanning electron microscopy. This work should be beneficial for a better understanding of protein aggregation.

Determination of anthraquinones in urine samples by pH-dependent solidified floating liquid-liquid microextraction-HPLC

DU Xuehong,XIN Xiaoying,WANG Jipeng,ZHANG Hongyi,YANG Bing

2018, 38(6):  596-602.  DOI: 10.3969/j.issn.1000-1565.2018.06.007

Asbtract ( 478 )   PDF (1501KB) ( 162 )  

References | Related Articles | Metrics

A method for the determination of anthraquinones in urine was developed by the combination of pH - dependent solidified floating liquid-liquid microextraction with HPLC. Octanoic acid chosen as extraction solvent was dissolved in alkaline solution, and 100 μL of it was taken and injected into 10 mL aqueous solution which had been acidified. After centrifugation for 4 min and ice bath for 2 min, the extract was floated on the surface of the aqueous sample. The extract was taken out and dissolved with 50 μL methanol for HPLC analysis. The main factors, such as acid type, sodium octanate volume, ion strength and centrifugal time, were systematically investigated. In the optimal conditions, there was a good linear relationship of peak areas for emodin and chrysophanol with their concentrations in the range of 5—400 ng/mL(R² > 0.999),with detection limit 0.75—0.8 ng/mL, and the relative standard deviation- DOI:10.3969/j.issn.1000-1565.2018.06.007pH依赖型悬浮固化液-液微萃取-HPLC测定尿液中2种蒽醌类物质杜雪红, 辛晓莹,王计彭, 张红医,杨冰(河北大学 化学与环境科学学院,河北 保定 071002 )摘 要:建立了一种pH依赖型悬浮固化液-液微萃取-HPLC测定尿液中大黄素和大黄酚的方法.该方法选择辛酸钠为萃取剂前体,在加入酸性样品溶液的过程中原位形成萃取剂辛酸.将100 μL辛酸钠溶液注入到事先酸化的10 mL样品水溶液中,经离心和冰浴后,萃取液固化并浮于溶液上层,取出固化的萃取液并溶解于50 μL甲醇供HPLC分析使用.对作为萃取剂的酸种类、辛酸钠体积、离子强度、离心时间等重要因素进行了优化.在最优条件下,大黄素和大黄酚在5~400 ng/mL内与峰面积间存在良好的线性关系(R² > 0.999),检出限为0.75~0.8 ng/mL,相对标准偏差为2.0%~3.2%(n=5).将该方法应用于添加后的尿液样品分析,回收率为82%~110%.该法萃取过程中避免了有机分散溶剂的使用,萃取时间短,操作简单,成本低,绿色环保,非常适于与液相色谱分析联合使用.关键词:分散液-液微萃取;辛酸;蒽醌类;高效液相色谱法中图分类号:O652.1 文献标志码:A 文章编号:1000-1565(2018)06-0596-07Determination of anthraquinones in urine samples by pH-dependent solidified floating liquid-liquid microextraction-HPLCDU Xuehong,XIN Xiaoying,WANG Jipeng,ZHANG Hongyi,YANG Bing(College of Chemistry and Environmental Science, Hebei University, Baoding 071002,China)Abstract: A method for the determination of anthraquinones in urine was developed by the combination of pH - dependent solidified floating liquid-liquid microextraction with HPLC. Octanoic acid chosen as extraction solvent was dissolved in alkaline solution, and 100 μL of it was taken and injected into 10 mL aqueous solution which had been acidified. After centrifugation for 4 min and ice bath for 2 min, the extract was floated on the surface of the aqueous sample. The extract was taken out and dissolved with 50 μL methanol for HPLC analysis. The main factors, such as acid type, sodium octanate volume, ion strength and centrifugal time, were systematically investigated. In the optimal conditions, there was a good linear relationship of peak areas for emodin and chrysophanol with their concentrations in the range of 5—400 ng/mL(R² > 0.999),with detection limit 0.75—0.8 ng/mL, and the relative standard deviation- 收稿日期:2018-02-18 基金项目:教育部留学回国人员科研启动基金(47批);河北大学校内科研项目(799207022;799207217014);河北大学2018年校级大学生创新创业计划项目及河北大学创新资助项目(hbu2018ss21) 第一作者:杜雪红(1989—),女,河北邯郸人,河北大学在读硕士研究生.E-mail:1043267494@qq.com 通信作者:张红医(1967—),男,河北保定人,河北大学教授,主要从事色谱、样品前处理和识别分析.E-mail:freetime20100401@163.com第6期杜雪红等:pH依赖型悬浮固化液-液微萃取-HPLC测定尿液中2种蒽醌类物质2.0%~3.2%(n=5). The method was applied to the analysis of urine samples, and the recovery after spiking was in the range of 82% and 110%. The extraction process avoids the use of organic dispersive solvents. The advantages of the proposed method are short extraction time, simple operation, low consumption and friendly environment, and very suitable for the combination with liquid chromatography analysis.

Influence of supports on the catalytic activity and stability of NiB nanocatalyst in the hydrogenation of cinnamic acid

HAN Xue, FENG Wenhui, NIE Shilin, LAN Xingwang

2018, 38(6):  603-609.  DOI: 10.3969/j.issn.1000-1565.2018.06.008

Asbtract ( 511 )   PDF (1658KB) ( 240 )  

References | Related Articles | Metrics

A series of supported NiB nanocatalysts by an impregnation-reduction method were prepared and evaluated in the hydrogenation of cinnamic acid, and the effect of different supports(γ-Al₂O₃, SiO₂, TiO₂)on the catalytic activity and stability was investigated. It is found that the support γ-Al₂O₃ showed a better dispersion effect on nickel active species, and the NiB/γ-Al₂O₃ catalyst had a less loss of active species after several runs. The catalytic activity of NiB/γ-Al₂O₃ catalyst in the hydrogenation of cinnamic acid showed the best stability. Notably, the NiB/γ-Al₂O₃ catalyst was gradually abnormally increased after three runs, and the conversion of cinnamic acid was enhanced to 75.0% after three times, the selectivity of hydrogenation product was kept in 100%. It may be due to that active species NiB in channels of support are constantly exposed during the reaction process, and the support can effectively hinder the loss- DOI:10.3969/j.issn.1000-1565.2018.06.008载体对纳米NiB催化肉桂酸加氢催化活性与稳定性的影响韩雪^1,2,冯文慧²,聂世琳²,兰兴旺²(1.河北省地质实验测试中心,河北 保定 071051;2.河北大学 化学与环境科学学院,河北 保定 071002)摘 要:采用浸渍-还原法制备负载型纳米NiB催化剂,以肉桂酸加氢反应为模型,考察载体(γ-Al₂O₃、SiO₂、TiO₂)对催化剂的催化活性和稳定性的影响.发现载体γ-Al₂O₃具有较好的分散镍活性组分的作用,在多次循环使用后催化剂活性组分流失较少.负载型NiB/γ-Al₂O₃催化剂在肉桂酸加氢反应中表现出较好的稳定性,此外,催化剂套用次数越多其活性反而越高,连续反应3次后肉桂酸的转化率可达75.0%,其加氢产物的选择性仍然保持在100%.通过系列表征,发现其最主要的原因可归为随着反应的不断进行载体孔道中的活性组分纳米NiB逐步暴露所致,更重要的是载体γ-Al₂O₃能够有效抑制活性组分的流失和团聚.关键词:γ-Al₂O₃;纳米NiB催化剂;稳定性;肉桂酸;加氢中图分类号:O643 文献标志码:A 文章编号:1000-1565(2018)06-0603-07Influence of supports on the catalytic activity and stability of NiB nanocatalyst in the hydrogenation of cinnamic acidHAN Xue^1,2, FENG Wenhui², NIE Shilin², LAN Xingwang²(1. Hebei Geological Experimental Testing Center, Baoding 071051, China;2. College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China)Abstract: A series of supported NiB nanocatalysts by an impregnation-reduction method were prepared and evaluated in the hydrogenation of cinnamic acid, and the effect of different supports(γ-Al₂O₃, SiO₂, TiO₂)on the catalytic activity and stability was investigated. It is found that the support γ-Al₂O₃ showed a better dispersion effect on nickel active species, and the NiB/γ-Al₂O₃ catalyst had a less loss of active species after several runs. The catalytic activity of NiB/γ-Al₂O₃ catalyst in the hydrogenation of cinnamic acid showed the best stability. Notably, the NiB/γ-Al₂O₃ catalyst was gradually abnormally increased after three runs, and the conversion of cinnamic acid was enhanced to 75.0% after three times, the selectivity of hydrogenation product was kept in 100%. It may be due to that active species NiB in channels of support are constantly exposed during the reaction process, and the support can effectively hinder the loss- 收稿日期:2018-07-01 基金项目:国家自然科学基金资助项目(21676068);河北省自然科学基金资助项目(B2018201118) 第一作者:韩雪(1983—),女,河北保定人,河北省地质实验测试中心工程师,主要从事绿色合成催化及分析测试研究.E-mail: yukixuer@163.com 通信作者:兰兴旺(1989—),男,河北唐山人,河北大学副教授,博士,主要从事纳米催化剂设计及二氧化碳转化反应和绿色合成研究.E-mail: hxlxw@sina.cn第6期韩雪等:载体对纳米NiB催化肉桂酸加氢催化活性与稳定性的影响and aggregation of active species.

Preparation and pharmacokinetics of aripiprazole long-acting injectable suspension

LI Xin,LIU Haofan,YANG Han,ZHAO Na,ZHANG Donghao,HU Liandong

2018, 38(6):  610-616.  DOI: 10.3969/j.issn.1000-1565.2018.06.009

Asbtract ( 738 )   PDF (2115KB) ( 375 )  

References | Related Articles | Metrics

To prepare aripiprazole long-acting injectable suspension and study its pharmacokinetics in rabbits. Suspension was prepared by high-pressure homogenization and the product was obtained after freeze-drying; The concentration of drug in plasma was determined by high performance liquid chromatography. The long-acting injectable suspension formulation obtained was uniform and stable in particle size and had no significant irritation to animal tissues. The in vivo pharmacokinetics results showed that the long-acting injection has a good sustained-release effect in vivo and can be sustainedly released for 15 days. Aripiprazole long-acting injectable suspension have controllable quality and can be released continuously and steadily, which is expected to provide a convenient, safe and effective new dosage form for schizophrenic patients.

Chemical constituents and antioxidant activity of Dracocephalum rupestre Hance

HAO Na, MA Weiwei, HUANG Hangjun, LI Jianfei, WANG Junli

2018, 38(6):  617-622.  DOI: 10.3969/j.issn.1000-1565.2018.06.010

Asbtract ( 601 )   PDF (1259KB) ( 302 )  

References | Related Articles | Metrics

For the utilization of the resources of Dracocephalum rupestre Hance, the content of total phenol and total flavonoids in different polar extracts was determined. The results showed that the content of total phenol and total flavonoids in ethyl acetate fraction was 74.5 mg/g and 426.70 mg/g, respectively, much higher than other fractions. Six compounds, eriodictyol, komaroviquinone, pedalitin, dracocequinone A, gardenin B and cirsimaritin were identified from the samples by LC-MS. In vitro antioxidant analysis showed that the D. rupestre Hance had strong antioxidant activity.

Antagonistic effect of Streptomyces microflavus Sy11 strain induced by Phytophthora infestans

JIANG Jizhi,WANG Xuening, LI Chengbin, ZHANG Hehua, LI Bing

2018, 38(6):  623-631.  DOI: 10.3969/j.issn.1000-1565.2018.06.011致病疫霉诱导放线菌Sy11菌株的抑菌作用

Asbtract ( 419 )   PDF (2649KB) ( 180 )  

References | Related Articles | Metrics

Actinomycetes Streptomyces microflavus Sy11 strain had strong suppression to mycelial growth and sporangia germination of Phytophthora infestans. And it was found that suppression was induced by P. infestans during antagonists screening in early experiments. In order to further reveal the induction effect and conditions of P. infestans to strain Sy11, dual culture method was used for investigating the suitable culture medium, temperature, time, and continual inhibition of Sy11 strain after induction. The results showed that only the alive P. infestans could induce the inhibition effect of Sy11, dead P. infestans could not; The strongest restrain of Sy11 was found on Rye medium, 20 ℃ for culture 7 d in dark, with free cell agar block which was 12 mm from Sy11 colony edge, the inhibition rate reached 79.38% and- DOI:10.3969/j.issn.1000-1565.2018.06.011致病疫霉诱导放线菌Sy11菌株的抑菌作用蒋继志¹,王雪宁¹,李成斌¹,张荷花¹,李兵²(1.河北大学 生命科学学院,河北 保定 071002;2.邢台医学高等专科学校 基础医学部,河北 邢台 054000)摘 要:在筛选致病疫霉拮抗菌过程中,发现放线菌Sy11菌株对致病疫霉的抑制作用是由于致病疫霉对Sy11的诱导作用.为了进一步明确致病疫霉对Sy11的诱导方式和条件,采用平板对峙共培养法探讨了致病疫霉诱导Sy11的适宜培养基、培养温度、培养时间以及诱导后Sy11菌株抑菌作用的持续时间等.结果显示,只有活体致病疫霉才能诱导Sy11的抑菌作用,死亡的致病疫霉菌体没有诱导作用;用黑麦培养基20 ℃黑暗对峙培养7 d,从Sy11菌落向外12 mm处的无菌体琼脂块的抑菌作用最强,抑菌率达到79.38%,与Sy11菌株完整菌落的抑菌率86.47%相近;而且发现致病疫霉诱导1次后,Sy11的抑菌能力即可连续传递13代,且抑菌率保持在65%以上.这些结果表明,Sy11是在受到活体致病疫霉诱导之后才对致病疫霉有抑制作用,且抑菌作用具有遗传性.关键词:致病疫霉;放线菌;相互感应;抑菌作用;诱导中图分类号:S435.32 文献标志码:A 文章编号:1000-1565(2018)06-0623-09Antagonistic effect of Streptomyces microflavus Sy11 strain induced by Phytophthora infestansJIANG Jizhi¹,WANG Xuening¹, LI Chengbin¹, ZHANG Hehua¹, LI Bing²(1.College of Life Sciences,Hebei University,Baoding 071002,China;2. Ministry of Basic Medicine,Xingtai Medical College, Xingtai 054000,China )Abstract:Actinomycetes Streptomyces microflavus Sy11 strain had strong suppression to mycelial growth and sporangia germination of Phytophthora infestans. And it was found that suppression was induced by P. infestans during antagonists screening in early experiments. In order to further reveal the induction effect and conditions of P. infestans to strain Sy11, dual culture method was used for investigating the suitable culture medium, temperature, time, and continual inhibition of Sy11 strain after induction. The results showed that only the alive P. infestans could induce the inhibition effect of Sy11, dead P. infestans could not; The strongest restrain of Sy11 was found on Rye medium, 20 ℃ for culture 7 d in dark, with free cell agar block which was 12 mm from Sy11 colony edge, the inhibition rate reached 79.38% and- 收稿日期:2018-01-31 基金项目:河北省自然科学基金资助项目(C 2015201231);公益性行业(农业)科研专项(201303018) 第一作者:蒋继志(1960—),男,宁夏中宁人,河北大学教授,博士生导师,主要从事植物病害生物防治及其分子机理研究.E-mail:jizhijiang909@163.com 通信作者:李兵(1978—),男,河北邢台人,邢台医学高等专科学校讲师,主要从事生物化学与药学研究.E-mail:liyanhd81@163.com第6期蒋继志等:致病疫霉诱导放线菌Sy11菌株的抑菌作用was close to that of whole colony of Sy11 strain(86.47%). In addition, prohibition capability of Sy11 could be continuously transmited for 13 generations after only once induction, and inhibition rate could remain above 65%. These findings suggest only alive P. infestans can induce the inhibition effect of Sy11, and the capability of Sy11 has inheritance.

Inhibitory effect of lipopeptide crude extract produced by Bacillus subtilis WL2 on Phytophthora infestans and its isolation and identification

WU Yanqing, WANG Youyou, WANG Chang, SHA Mengying

2018, 38(6):  632-639.  DOI: 10.3969/j.issn.1000-1565.2018.06.012枯草芽孢杆菌WL2脂肽粗提物对致病疫霉

Asbtract ( 522 )   PDF (1352KB) ( 243 )  

References | Related Articles | Metrics

In order to clarify the effect of lipopeptide secreted by Bacillus subtilis WL2 on Phytophthora infestans and the prevention potential on potato late blight, the paper strip method was adopted to evaluate the inhibitory activity of lipopeptide crude extract on mycelial growth and spores germination of P. infestans. In addition, in vitro tissue method was used to evaluate the disease prevention effect of lipopeptide crude extract. And the types of lipopeptide were also identified by ESI-MS.The results showed that when the concentration of lipopeptide crude extract was at 25 μg/mL, the diameter of inhibitory zone was 5.9 mm and mycelial recovery growth rate of P. infestans after inhibition was only 5.2 mm/d. There was- DOI:10.3969/j.issn.1000-1565.2018.06.012枯草芽孢杆菌WL2脂肽粗提物对致病疫霉的抑制作用及其分离鉴定吴艳清¹,王游游²,王畅¹,沙梦莹¹(1.保定学院 生物化工与环境工程学院,河北 保定 071000;2.河北大学 生命科学学院,河北 保定 071002)摘 要:为明确枯草芽孢杆菌(Bacillus subtilis)WL2产生的脂肽对致病疫霉菌丝体生长的影响及其预防马铃薯晚疫病的潜力,以滤纸片法检测脂肽粗提物对菌丝生长的抑制效果,显微镜观察脂肽粗提物对致病疫霉孢子萌发的影响,离体组织培养法明确脂肽粗提物对晚疫病的预防效果,高效液相色谱法(HPLC)纯化脂肽、电子喷雾质谱(ESI-MS)鉴定脂肽种类.结果表明,当脂肽粗提物质量浓度为25 μg/mL时,抑菌圈直径为5.9 mm,抑制后的菌丝恢复生长速度仅有5.2 mm/d,与对照组(11.2 mm/d)存在显著差异(P<0.05);当脂肽质量浓度为3 μg/mL时,孢子囊萌发比率只有9.7%,用质量浓度为4 μg/mL的脂肽抑制后,释放游动孢子比率只有7.6%,与对照组均存在显著差异(P<0.05);脂肽粗提物(20 μg/mL)对晚疫病的预防效果最好,病情指数最低(20.6),与对照组病情指数(73.1)有显著差异(P<0.05);经HPLC分离和ESI-MS鉴定,明确了WL2菌株可产iturinA和surfactin两类脂肽物质.以上结果说明WL2菌株产生的脂肽在预防马铃薯晚疫病方面具有较大的潜力.关键词:枯草芽孢杆菌;脂肽;抑制;致病疫霉;鉴定中图分类号:S435.32 文献标志码:A 文章编号:1000-1565(2018)06-0632-08Inhibitory effect of lipopeptide crude extract produced by Bacillus subtilis WL2 on Phytophthora infestans and its isolation and identificationWU Yanqing¹, WANG Youyou², WANG Chang¹, SHA Mengying¹(1.College of Biochemical and Environmental Engineering, Baoding University, Baoding 071000, China; 2. College of Life Sciences, Hebei University, Baoding 071002, China)Abstract: In order to clarify the effect of lipopeptide secreted by Bacillus subtilis WL2 on Phytophthora infestans and the prevention potential on potato late blight, the paper strip method was adopted to evaluate the inhibitory activity of lipopeptide crude extract on mycelial growth and spores germination of P. infestans. In addition, in vitro tissue method was used to evaluate the disease prevention effect of lipopeptide crude extract. And the types of lipopeptide were also identified by ESI-MS.The results showed that when the concentration of lipopeptide crude extract was at 25 μg/mL, the diameter of inhibitory zone was 5.9 mm and mycelial recovery growth rate of P. infestans after inhibition was only 5.2 mm/d. There was- 收稿日期:2018-04-10 基金项目:河北省青年自然科学基金资助项目(C2015104014);保定学院博士基金资助项目(2013Z01) 第一作者:吴艳清(1982—),男,河北河间人,保定学院讲师,博士,主要从事微生物资源开发及应用研究.E-mail:wuyanqing198212@126.com 通信作者:王游游(1992—),男,河北石家庄人,河北大学硕士研究生,主要从事植物病害生物防治研究.E-mail:15032257263@163.com第6期吴艳清等:枯草芽孢杆菌WL2脂肽粗提物对致病疫霉的抑制作用及其分离鉴定significant difference in comparison with control(11.2 mm/d, P<0.05). In addition, spores direct germination rate was 9.7% after inhibition with lipopeptide crude extract(3 μg/mL)and zoospore release rate was only 7.6% after inhibition with of lipopeptide crude extract(4 μg/mL). There were differences in comparison with control(P<0.05). In addition, lipopeptide crude extract(20 μg/mL)exhibited the best effect on disease prevention of potato late blight, and the disease index(20.6)was the lowest. There were differences in comparison with control(73.1, P<0.05). The lipopeptide produced by WL2 strain were iturinA and surfactin. These results indicate that lipopeptide produced by WL2 strain has great potential in controlling potato late blight in future.

Shilling attacks and security of recommender systems

TIAN Junfeng, CAI Hongyun

2018, 38(6):  640-647.  DOI: 10.3969/j.issn.1000-1565.2018.06.013

Asbtract ( 690 )   PDF (1116KB) ( 444 )  

References | Related Articles | Metrics

As an effective solution for solving the problem of information overload, collaborative recommender systems have been widely applied to many fields. However, the occurrence of shilling attacks has seriously affected the quality of recommendation. Therefore, how to protect recommender systems against shilling attacks has become hotspot issue in recommender systems. This paper first introduces the motivation of shilling attacks and features of various attack models, and then analyzes the state of the art in shilling attacks detection techniques and robust collaborative recommendation algorithms in detail. Finally, the future development and research directions are discussed.

CT beam hardening correction method based on projection space

ZHANG Xiaoke, YANG Kun, CHEN Jintao, XUE Linyan, LI Xiaowei

2018, 38(6):  648-655.  DOI: 10.3969/j.issn.1000-1565.2018.06.014

Asbtract ( 511 )   PDF (1410KB) ( 440 )  

References | Related Articles | Metrics

X ray spectrum of medical CT imaging system is polychromatic, which will cause CT image hardening artifacts, reduce image quality and interfere diagnosis.Therefore,the correction of the hardening artifacts of CT images is necessary. In this paper, a polynomial fitting correction method is proposed based on projection space optimization for single medium water and water bone mixture. Firstly, the projection correction model and the ideal projection model are constructed. Then, the projection correction model is compared with the ideal projection model to determine the correction coefficient when the difference is minimum. Finally, the correction coefficient is substituted into the projection correction model,and the corrected image can be reconstructed. The simulation results show that the proposed polynomial fitting correction method can remove CT hardening artifacts and improve the image accuracy, which is of great significance in the accurate quantitative analysis of CT.

Life cycle Environmental impact assessment of a closed-loop LED lamp supply chain

LONG Zhanlu, WU Jinzhuo, XU Wenxiu, XUE Jinran

2018, 38(6):  656-663.  DOI: 10.3969/j.issn.1000-1565.2018.06.015

Asbtract ( 452 )   PDF (1478KB) ( 437 )  

References | Related Articles | Metrics

Based on the theory of Life Cycle Assessment and SimaPro software(Version8.0), the Eco-indicator 99 method was used to analyze the life cycle impact of a closed-loop LED lamp supply chain and the results were compared with an open-loop LED lamp supply chain which has the same function unit. Results indicated that the environmental single score of a 21 W LED lamp in the entire process of the closed-loop supply chain was 5.48 Pt. The descending order of the three categories of environmental impact was: human health(3.81 Pt)> resources consumption(1.29 Pt)> ecological environment(0.381 Pt), indicating that the whole process of the closed-loop LED lamp supply chain had the most significant impact on human health. In addition, the environmental single score of the open-loop LED lamp supply chain with the same function unit was 5.71 Pt, increasing by 0.23 Pt compared with the closed-loop supply chain, which indicated that the closed-loop LED lamp supply chain was much more environmental friendly than the open-loop LED lamp supply chain.

Phylogenetic Diversity of heavy metal-resistant bacteria in Baiyangdian Lake and its relationship with ecological factors

CHEN Siyu, WANG Lina, YANG Huiqi, CHEN Yu, ZHANG Jin, LÜ Zhitang

2018, 38(6):  664-672.  DOI: 10.3969/j.issn.1000-1565.2018.06.016

Asbtract ( 499 )   PDF (1979KB) ( 205 )  

References | Related Articles | Metrics

In order to provide theoretical support and bacterial resources for the treatment of heavy metal pollution in Baiyangdian Lake, the phylogenetic diversity of heavy metal-resistant bacteria in Baiyangdian was studied using isolation and culture method, and the relationship between the distribution of heavy metal-resistant bacteria and ecological factors was analyzed. 54 strains of anti-80 mg/L Pb²⁺ bacteria, 46 strains of anti-80 mg/L Cu²⁺ bacteria, and 39 strains of anti-100 mg/L Cr(Ⅵ)bacteria were isolated- DOI:10.3969/j.issn.1000-1565.2018.06.016白洋淀抗重金属细菌多样性及其与生态因子相关性陈思雨¹, 王丽娜¹, 杨惠琪¹, 陈玉¹, 张瑾¹, 吕志堂^1,2(1.河北大学 生命科学学院 河北省微生物多样性研究与应用实验室, 河北 保定 071002;2.药物化学与分子诊断教育部重点实验室, 河北 保定 071002)摘 要:为了给白洋淀重金属污染治理提供理论支持和菌种资源, 采用分离培养的方法调查了白洋淀抗重金属细菌的系统发育多样性,并分析了抗重金属细菌分布与生态因子之间的关系. 从各采样点的样品中分离筛选得到抗80 mg/L Pb²⁺细菌54株、抗80 mg/L Cu²⁺细菌46株, 抗100 mg/L Cr(Ⅵ)细菌39株. 综合考虑各菌株的抗性及16S rRNA基因序列差异,将139株抗重金属菌去除重复后合并为69株, 分属于肠杆菌属(Enterobacter)、丛毛单胞菌属(Comamonas)、戴尔福特菌属(Delftia)、短波单胞菌属(Brevundimonas)、寡养单胞菌属(Stenotrophomonas)、假单胞菌属(Pseudomonas)和赖氨酸芽孢杆菌属(Lysinibacillus)共7个属13个种. 进一步采用PCA分析的方法对抗重金属细菌与生态因子相关性进行研究, 发现在菌群组成和分布范围方面抗Pb²⁺和Cu²⁺菌大体相似, 且假单胞菌属和寡养单胞菌属2个属容限最高, 表明菌株对于重金属具有交叉抗性;抗100 mg/L Cr(Ⅵ)细菌中赖氨酸杆菌属为特有属, 且分布广泛;白洋淀水域唯一能够同抗Pb²⁺、Cu²⁺和Cr(Ⅵ)3种重金属的细菌是寡养单胞菌属.关键词:白洋淀;抗重金属细菌;系统发育多样性;生态因子中图分类号:Q938.1+1 文献标志码:A 文章编号:1000-1565(2018)06-0664-09Phylogenetic Diversity of heavy metal-resistant bacteria in Baiyangdian Lake and its relationship with ecological factors CHEN Siyu¹, WANG Lina¹, YANG Huiqi¹, CHEN Yu¹, ZHANG Jin¹, LÜ Zhitang^1,2(1. Key Laboratory of Microbial Diversity Research and Application of Hebei Province,College of Life Sciences, Hebei University, Baoding 071002, China;2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China)Abstract: In order to provide theoretical support and bacterial resources for the treatment of heavy metal pollution in Baiyangdian Lake, the phylogenetic diversity of heavy metal-resistant bacteria in Baiyangdian was studied using isolation and culture method, and the relationship between the distribution of heavy metal-resistant bacteria and ecological factors was analyzed. 54 strains of anti-80 mg/L Pb²⁺ bacteria, 46 strains of anti-80 mg/L Cu²⁺ bacteria, and 39 strains of anti-100 mg/L Cr(Ⅵ)bacteria were isolated- 收稿日期:2018-05-20 基金项目:河北省生物学强势特色学科项目(1050-5030004);河北省生物工程重点学科项目(1050-5030023) 第一作者:陈思雨(1994—),女,河北安新人,河北大学在读硕士研究生. E-mail:331255943@qq.com 通信作者:吕志堂(1973—),男,河北曲周人,河北大学教授,博士,主要从事微生物资源与系统学研究. E-mail:lzt325@126.com第6期陈思雨等:白洋淀抗重金属细菌多样性及其与生态因子相关性and screened from the sampling sites. A total of 69 strains were combined after repeated strains were removed based on heavy metal-resistant characteristics sequence difference, which belonged to 13 species distributed in 7 genera Enterobacter, Comamonas, Delftia, Brevundimonas, Stenotrophomonas, Pseudomonas, and Lysinibacillus. Further PCA analysis was used to study the relationship between heavy metal resistant bacteria and ecological factors. In terms of group composition and distribution, the anti-Pb²⁺ and Cu²⁺ bacteria were generally similar, and Pseudomonas and Stenotrophomonas had the highest abundance, indicating that the strain had cross-resistance to heavy metals. Lysinibacillus is the unique 100 mg/L Cr(Ⅵ)resistant genus, and it is widely distributed. Stenotrophomonas is the only genus which can survive in three kinds of heavy metals Pb²⁺, Cu²⁺ and Cr(Ⅵ)within Baiyangdian Lake.