蜗牛肠道细菌及其纤维素酶性质

doi:10.3969/j.issn.1000-1565.2017.04.011蜗牛肠道细菌及其纤维素酶性质

河北大学学报(自然科学版) ›› 2017, Vol. 37 ›› Issue (4): 400-404.DOI: 10.3969/j.issn.1000-1565.2017.04.011蜗牛肠道细菌及其纤维素酶性质

蜗牛肠道细菌及其纤维素酶性质

昌艳萍,田其凡,郭欣,李彦芹,闫蕾蕾,李春青

收稿日期:2016-12-27 出版日期:2017-07-25 发布日期:2017-07-25
作者简介:昌艳萍(1969—),女,湖北仙桃人,河北大学副教授,博士,主要从事微生物技术的研究. E-mail:yanpingchang@126.com
基金资助:
河北大学博士基金资助项目(2014-276)

Composition of the intestinal bacteria and their cellulase characterization in Cathaica fasciola

CHANG Yanping, TIAN Qifan, GUO Xin, LI Yanqin, YAN Leilei, LI Chunqing

College of Life Sciences, Hebei University, Baoding 071002, China

Received:2016-12-27 Online:2017-07-25 Published:2017-07-25

摘要/Abstract

摘要： 为了探寻功能微生物,用几种不同培养基分离蜗牛肠道可培养细菌并筛选纤维素降解细菌,研究了纤维素酶学性质.结果表明,LB培养基培养蜗牛肠道细菌时菌落种类和数量最多,达3.3×10⁴ CFU/mL.刚果红染色显示,7种优势细菌全部具有产酶能力;菌S6粗酶液的内切葡聚糖酶(Cx)和滤纸酶(FPase)活性最高,分别为5.96和2.84 U/mL.对Cx酶学性质初步研究表明:该酶最适反应温度为35 ℃,在55 ~75 ℃时有较高的热稳定性;最适pH为6.0,在pH 3.0~8.0均有酶活力,pH 8.0相对酶活仍为60%;Mg²⁺、Na⁺、K⁺和Ca²⁺对酶反应具有促进作用,Zn²⁺、Cu²⁺和Mn²⁺为酶反应的抑制剂.研究蜗牛肠道细菌的组成,从中分离高产纤维素酶菌株,为纤维素生物降解转化成可再生能源寻找新的思路.

关键词: 条华蜗牛, 肠道细菌, 产酶能力, 纤维素生物降解

Abstract: In order to explore the functional microorganism, the intestinal culturable microflora in Cathaica fasciola were screened and cultured by several kinds of culture media, and the cellulase activity of the isolated bacteria and the characteristics of the crude cellulase that produced by strain S6 was studied.The results showed that the largest number of intestinal bacteria was up to 3.3×10⁴ CFU/mL in LB medium.Congo red staining showed that all the 7 dominant bacteria had the ability to secrete cellulase.The strain S6 had highest CMCase and FPase values of 5.96 and 2.84 U/mL, respectively.The CMCase characterization revealed that, the optimum reaction temperature was 35 ℃, the cellulose kept its high activity from 55 ℃to 75 ℃.The crude enzyme still had 60% of the highest obvious activation function at pH 8.0.Mg²⁺、Na⁺、K⁺and Ca²⁺ had enhancing effect, and Zn²⁺、Cu²⁺ and Mn²⁺ had inhibitory action.To research the composition of the snail intestinal bacteria and separate high-yield cellulase strains is a new way for the cellulose biodegradation into renewable energy.

Key words: Cathaica fasciola, intestinal bacteria, enzyme producing capabilities, cellulose biodegradation

中图分类号:

Q939.9

昌艳萍,田其凡,郭欣,李彦芹,闫蕾蕾,李春青. 蜗牛肠道细菌及其纤维素酶性质[J]. 河北大学学报(自然科学版), 2017, 37(4): 400-404.

CHANG Yanping, TIAN Qifan, GUO Xin, LI Yanqin, YAN Leilei, LI Chunqing. Composition of the intestinal bacteria and their cellulase characterization in Cathaica fasciola[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(4): 400-404.

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蜗牛肠道细菌及其纤维素酶性质

Composition of the intestinal bacteria and their cellulase characterization in Cathaica fasciola

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