超高效液相色谱测定肉制品中的罗丹明B、孔雀石绿和结晶紫及其代谢物残留

doi:10.3969/j.issn.1000-1565.2013.02.009

摘要/Abstract

摘要： 提出了一种以超高效液相色谱同时检测肉制品中5种违禁合成色素的新方法.以甲醇-水(V(甲醇)∶V(水)=95∶5)作为提取剂,样品在80℃经微波辅助萃取5 min,继以C18固相萃取柱净化,使用ACQUITY BEH C18分析柱,以乙腈-20 mmol/L乙酸铵缓冲溶液(V(乙腈)∶V(乙酸铵)=80∶20,pH=5)作流动相,实现了5种色素的有效分离.在0.1～5.0 μg/mL内,校准曲线呈良好的线性,相关系数r为0.994 5～0.999 5.该方法测定肉制品中罗丹明B、孔雀石绿、结晶紫、隐性孔雀石绿和隐性结晶紫的定量限分别为4.23,1.83,1.61,1.96,1.95 μg/kg.对加标50 μg/kg的牛肉香肠日内测定6次,5种分析物的精密度(以RSD表示)优于9.2％.在25 μg/kg和75 μg/kg添加水平,平均回收率为78.01％～109.2％,相对标准偏差小于10％.实验结果表明:该方法具有快速、灵敏和准确等优点,可用于肉制品中5种违禁色素的常规分析.

关键词: 违禁色素, 肉制品, 微波辅助提取, 固相萃取, 超高效液相色谱

中图分类号:

O657.7

孙娜,李挥,孙汉文. 超高效液相色谱测定肉制品中的罗丹明B、孔雀石绿和结晶紫及其代谢物残留[J]. 河北大学学报(自然科学版), 2013, 33(2): 154-160.

SUN Na,LI Hui,SUN Hanwen. Simultaneous development of rhodamine B, malachite green, crystal violet and their metabolites residues in meat by UPLC[J]. Journal of Hebei University (Natural Science Edition), 2013, 33(2): 154-160.

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