双核钌配合物的DNA键合及光断裂性能

doi:10.3969/j.issn.1000-1565.2019.03.006

摘要/Abstract

摘要： 合成了1个新型的双核钌配合物,并通过紫外-可见吸收光谱和荧光光谱滴定、热熔链、盐效应、黏度和凝胶电泳实验研究了其与小牛胸腺DNA(ct-DNA)之间的相互作用. 结果表明,由于主配体的位阻作用,该配合物通过部分插入模式与DNA键合,键合常数大于10⁶ L/mol;在365 nm紫外光照射下,该配合物能够断裂pBR322 DNA,是有效的DNA断裂试剂.

关键词: 钌配合物, DNA, 键合, 电泳

Abstract: A dinuclear ruthenium complex has been synthesized and characterized. The calf thymus DNA(ct-DNA)binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, DNA thermal denaturation and reverse, salt effect and agarose gel electrophoresis. The results suggest that the complex binds to DNA through partial-intercalation mode with a large DNA binding constant of 10⁶ L/mol order of magnitude. The complex is also demonstrated to be an efficient cleaver of pBR 322 DNA under irradiation at 360 nm.

Key words: ruthenium complex, DNA, bind, electrophoresis

中图分类号:

Q523

王爽,徐雪雪,米雅萱,郑泽宝,赵晓珑. 双核钌配合物的DNA键合及光断裂性能[J]. 河北大学学报(自然科学版), 2019, 39(3): 254-263.

WANG Shuang, XU Xuexue, MI Yaxuan, ZHENG Zebao, ZHAO Xiaolong. DNA binding and photocleavage properties of a dinuclear ruthenium complex[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(3): 254-263.

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