Abstract: To investigate the anti-tumor and immune function of compound Taisui oral liquid,(CTOL)in vivo and in vitro, the cytotoxic effects of different doses of CTOL on SGC7901 cells and Hela cells were detected in vitro. The inhibitory effect of CTOL in different concentrations on tumor cells proliferation was detected by MTT assay. S180 tumor-bearing mice were divided into six groups randomly, which are, model group, CTX group, low-dose of CTOL group, high-dose of CTOL group, low-dose of CTOL + CTX group and high-dose of CTOL + CTX group. CTX was intraperitoneally injected and different doses of CTOL were given by gavages. After 6 weeks of continuous administration,- DOI:10.3969/j.issn.1000-1565.2023.05.009复方太岁口服液体内外抗肿瘤及免疫调节作用 石炳烨^1,2,夏子涵²,王蓓²,靳文勋³(1.河北大学附属医院 超声诊断科,河北 保定 071000;2.河北大学 基础医学院,河北 保定 071000;3.保定脑血管病医院 神经外科,河北 保定 071000)摘 要:为探讨复方太岁口服液(compound Taisui oral liquid, CTOL)体内外抗肿瘤及对免疫功能的调节作用,体外采用MTT法检测不同浓度CTOL对SGC7901和Hela 细胞增殖的抑制作用,体内制备S180 荷瘤小鼠模型,实验分为模型组、CTX组、CTOL低剂量组、CTOL高剂量组、CTOL低剂量+CTX组、CTOL高剂量+CTX组.CTX采用腹腔注射给药,CTOL采用灌胃方式给药,连续给药6周后处死小鼠,称取瘤质量和脾质量,计算抑瘤率和脾指数,ELISA法检测血清IL-2和IFN-γ水平.结果显示:不同浓度CTOL对SGC7901和Hela细胞均有抑制作用,且呈剂量依赖关系.体内各实验组瘤质量均明显低于模型组(P<0.05),其中CTOL高剂量+CTX组的抑瘤率最高为70.90%;CTOL低、高剂量+CTX组的脾指数、IL-2、IFN-γ水平均明显高于CTX组(P<0.05).由此可得:CTOL在体内外均可抑制肿瘤的生长,并可改善CTX导致的脾脏损伤及促进IL-2和IFN-γ的分泌.关键词:复方太岁口服液;抗肿瘤;免疫功能中图分类号:R285.5 文献标志码:A 文章编号:1000-1565(2023)05-0506-06In vivo and in vitro anti-tumor and immunomodulation effect of compound Taisui oral liquid SHI Bingye^1,2,XIA Zihan²,WANG Bei²,JIN Wenxun³(1. Department of Ultrasound Diagnosis,Affiliated Hospital of Hebei University,Baoding 071000,China;2. School of Basic Medical Sciences,Hebei University, Baoding 071000,China; 3. Department of Neurosurgery,Baoding Cerebrovascular Disease Hospital,Baoding 071000,China)Abstract: To investigate the anti-tumor and immune function of compound Taisui oral liquid,(CTOL)in vivo and in vitro, the cytotoxic effects of different doses of CTOL on SGC7901 cells and Hela cells were detected in vitro. The inhibitory effect of CTOL in different concentrations on tumor cells proliferation was detected by MTT assay. S180 tumor-bearing mice were divided into six groups randomly, which are, model group, CTX group, low-dose of CTOL group, high-dose of CTOL group, low-dose of CTOL + CTX group and high-dose of CTOL + CTX group. CTX was intraperitoneally injected and different doses of CTOL were given by gavages. After 6 weeks of continuous administration,- 收稿日期:2022-10-25 基金项目:河北省医学科学研究课题计划(20211152);河北大学医学学科培育项目(2020B16); 2022年国家级大学生创新创业训练计划项目(202210075017) 第一作者:石炳烨(1983—),男,河北保定人,河北大学附属医院主治医师,河北大学在读硕士研究生,主要从事免疫药理研究.E-mail:2435231348@qq.com 通信作者:靳文勋(1978—),男,河北曲阳人,保定脑血管病医院副主任医师,主要从事神经肿瘤免疫研究.E-mail:jwx780602@163.com第5期石炳烨等:复方太岁口服液体内外抗肿瘤及免疫调节作用 mice were sacrificed, then tumor tissues and spleens were weighed. The tumor inhibition rate and spleen index were calculated and IL-2 and IFN-γ of serum were measured by ELISA. The proliferation of SGC7901 and Hela cells were inhibited by different concentrations of CTOL, and the cytotoxic effect of CTOL on different tumor cells was dose-dependent. The tumor weight of each experimental group was significantly lower than that of the model group(P<0.05), and the tumor inhibition rate of the high-dose of CTOL + CTX group was the highest(70.90%). The spleen index, IL-2 and IFN-γ levels in low-dose and high-dose of CTOL + CTX groups were significantly higher than those in the CTX group(P<0.05). CTOL can inhibit the growth of tumors in vitro and in vivo, promote the secretion of IL-2 and IFN-γ and has protective effect on the damage to spleen caused by CTX in tumor-bearing mice.

Key words: compound Taisui oral liquid, antitumor, immune function