Determination of vanilla mandelic acid and homovanillic acid based on capillay electrophoresis combinated with electrochemiluminescence detection

doi:10.3969/j.issn.1000-1565.2016.03.007

Abstract

Abstract: Vanilla mandelic acid(VMA)and homovanillic acid(HVA)in the urine were detected by capillary electrophoresis with electrochemiluminescence detection(CE-ECL).The following conditions were suitable for the determination of VMA and HVA:running buffer,50 mmol/L NaH₂PO₄-Na₂HPO₄(pH=8.2);separation voltage,16 kV;detection potential,1.2 V(vs.saturated calomel electrode(SCE)).Under the optimum conditions of detection,satisfactory sensitivities(LOD of 5.0×10^-7 mol/L for VMA and 6.1×10^-7 mol/L for HVA at S/N=3)were obtained,the correlation coefficients of the method were 0.999 3 and 0.997 9,respectively.The determination of these metabolites in human urine was completed within 10 min without any interferences,the recoveries were VMA 98% and HVA 99%,the result obtained is satisfactory.

Key words: capillary electrophoresis, electrochemiluminescence detection, vanilla mandelic acid, homovanillic acid

CLC Number:

O657.8

YANG Yang,SUN Xuemei,LI Chuanlong. Determination of vanilla mandelic acid and homovanillic acid based on capillay electrophoresis combinated with electrochemiluminescence detection[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(3): 257-263.

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