Separation and characterization of lipoproteins in serum using field flow fractionation

doi:10.3969/j.issn.1000-1565.2017.02.004

Abstract

Abstract: The lipoproteins in serum were separated and characterized using asymmetrical flow field-flow fractionation(AF4)coupled with multiangle light scattering(MALS),and fluorescence(FL)detectors.The effect of programmed cross-flow rates in linear decay and exponential decay on the AF4 analysis of serum was studied.The collected AF4 fractions of the serum were characterized by dynamic light scattering(DLS).It was found that the free proteins and lipoproteins in the serum were separated by AF4.The application of exponentially decaying cross-flow rate not only reduced the time of AF4 analysis of serum,but also improved the resolution for the sub-class of lipoproteins.The results demonstrate that AF4-MALS-FL is a useful tool for the analysis of lipoproteins in serum.

Key words: asymmetrical flow field-flow fractionation, serum, lipoprotein, coronary artery disease

CLC Number:

O657

WANG Jing,ZHANG Xiaoyue,ZHANG Jingwen,DOU Haiyang,SHEN Shigang. Separation and characterization of lipoproteins in serum using field flow fractionation[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(2): 128-133.

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