Establishment of regeneration system of Arisaema decipiens Schott

doi:10.3969/j.issn.1000-1565.2017.03.007

Abstract

Abstract: For protection and utilization of the wild Arisaema decipiens Schott,a regeneration system was developed.The effects of different plant growth regulators on callus,adventitious shoot and root induction were investigated by organogenesis using petiole as explants.The results showed that the optimal medium of callus induction was MS+6-BA 1.0 mg/L+2,4-D 1.0 mg/L.The suitable medium for adventitious shoots induction was MS+IAA 0.5 mg/L +6-BA 0.5 mg/L,and the induction rate of adventitious shoots reached at 82%.Up to 100% of the regenerated shoots formed complete plantlets on MS+IAA 0.5 mg/L+6-BA 0.5 mg/L medium.

Key words: Arisaema decipiens, callus, adventitious shoots, rooting culture

CLC Number:

Q945

ZHANG Yiming,LIN Yuxing,ZHANG Lu,XIAO Zefeng,WANG Junli. Establishment of regeneration system of Arisaema decipiens Schott[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(3): 262-266.

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