Chemical constituents and antioxidant activity of Dracocephalum rupestre Hance

doi:10.3969/j.issn.1000-1565.2018.06.010

Abstract

Abstract: For the utilization of the resources of Dracocephalum rupestre Hance, the content of total phenol and total flavonoids in different polar extracts was determined. The results showed that the content of total phenol and total flavonoids in ethyl acetate fraction was 74.5 mg/g and 426.70 mg/g, respectively, much higher than other fractions. Six compounds, eriodictyol, komaroviquinone, pedalitin, dracocequinone A, gardenin B and cirsimaritin were identified from the samples by LC-MS. In vitro antioxidant analysis showed that the D. rupestre Hance had strong antioxidant activity.

Key words: Dracocephalum rupestre Hance, total phenol, total flavonoids, LC-MS analysis, antioxidant

CLC Number:

Q945

HAO Na, MA Weiwei, HUANG Hangjun, LI Jianfei, WANG Junli. Chemical constituents and antioxidant activity of Dracocephalum rupestre Hance[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(6): 617-622.

References

[1] 中科院中国植物志编委会.中国植物志[M].科学出版社,1977,65(2):378.
[2] 任冬梅.两种青兰属植物的化学成分及活性研究[D].济南:山东大学,2007. REN D M. Chemical and bioactive studies on the species of the genus Dracocephalum[D]. Jinan:Shandong University, 2007.
[3] DASTMAICHI K, DORMAN H J D, LAAKSO L, et al. Chemical composition and in vitro antioxidative activity of a lemon balm(Melissa officinalis L.)extract[J]. LWT-Food Science and Technology, 2008, 41(3): 391-400. DOI: org/10.1016/j.lwt.2007.03.007.
[4] JAHANIANI F, EBRAHIMI S A, ROSHANDEL N R, et al. Xanthomicrol is the main cytotoxic component of Dracocephalum kotschyii and a potential anti-cancer agent[J]. Phytochernistry, 2005, 66(4): 1581-1592. DOI: org/10.1016/j.phytochem.2005.04.035.
[5] 海平.藏药唐古特青兰抗缺氧作用研究[J].山东中医杂志,2005, 24(1):41-43.DOI:10.16295/j.cnki.0257-358x.2005.01.024. HAI P. Study on anti-hypoxia effect of Tibetan medicine Dracocephalum tanguticum [J]. Shandong Journalof Traditional Chinese Medicine, 2005, 24(1):41-43.DOI:10.16295/j.cnki.0257-358x.2005.01.024.
[6] 洪秀芳,魏妤,刘文杰.香青兰对缺血心肌保护作用的实验研究[J]. 新疆医学院学报, 1997,20(2):95-98. HONG X F, WEI Y, LIU W J. Experimental study on the protective effect of Dracocephalum moldavica on ischemic myocardium [J]. Journal of Xinjiang Medical University, 1997,20(2):95-98.
[7] 王晓雯,依巴岱提,李迪民,等.香青兰提取物对心肌缺血的保护作用[J].中草药,1991,22(2):76-77. WANG X W, YI BADAITI, LI D M, et al. Protective effect of Dracocephalum moldavica extract on ischemic myocardium [J]. Chinese Traditional and Herbal Drugs, 1991,22(2):76-77.
[8] 李建飞, 王俊丽, 公维镇, 等.银莲花的抗氧化、抑菌和抗肿瘤活性[J].河北大学学报(自然科学版),2014,34(4):414-420. DOI: 10.3969/j.issn.1000-1565.2014.04.015. LI J F, WANG J L, GONG W Z, et al. Antioxidation, antibacterial and antitumor activities of Anemone cathayensis[J]. Journal of Hebei Universiry(Natural Science Edition), 2014, 34(4):414-420. DOI: 10. 3969/j.issn.1000-1565.2014.04.015.
[9] VALKO M,LEIBFRITZ D,MONCOL J,et al.Free radicals and antioxidants in normal physiological functions and human disease[J].The International Journal of Biochemistry & Cell Biology, 2007, 39:44-84.DOI: ORG/10.1016/J.BIOCEL.2006.07.001.
[10] 刘根生,徐贵发.丹参酚酸的抗氧化作用[J].食品与药品,2007, 9(1):69-71. LIU G S, XU G F. Antioxidant effect of salvianolic acid [J].Food and Drug, 2007, 9(1):69-71.
[11] 张改平,朱华泽,杨建雄.甘草提取物的体外抗氧化活性[J].生物加工过程,2010,8(3):53-57.DOI: 10.3969/j.issn.1672-3678.2010.03.011. ZHANG G P, ZHU H Z, YANG J X. In vitro antioxidant activity of extract from Glycyrrhiza uralensis[J]. Chinese Journal of Bioprocess Engineering, 2010,8(3):53-57.DOI: 10.3969/j.issn.1672-3678.2010.03.011.
[12] 宋月英,马玉珍.红景天属植物化学成分及药理作用的研究进展[J].中草药,2004,35(2):235-237. SONG Y Y,MA Y Z.Research advances on chemical constituents and pharmacological effects of the species of the genus Rhodiola [J]. Chinese Traditional and Herbal Drugs, 2004,35(2):235-237.
[13] WANG J L, LIU K,LI X X, et al. Variation of active constituents and antioxidant activity in Scabiosa tschiliensis Grunning from different stages [J]. Journal of Food Science and Technology, 2017, 54(8):2288-2295. DOI: 10.1007/s13197-017-2666-9.
[14] DASTMALCHI K, DORMANA H J D, KOSAR M, et al. Chemical composition and in vitro antioxidant evaluation of a water-soluble Moldavian balm(Dracocephalum moldavica L.)extract [J]. LWT-Food Science and Technology, 2007,40(2):239-248. DOI: org/10.1016/j.lwt.2005.09.019.