Tissue culture of Ligularia rumicifolia

doi:10.3969/j.issn.1000-1565.2021.01.008

Abstract

Abstract: For utilization of Ligularia rumicifolia(Drumm.)S. W. Liu, tissue culture in vitro was tested using cotyledon as explants. The results showed that the MS medium containing TDZ 1.0 mg/L and NAA 1.0 mg/L was suitable for callus induction. The maximum induction rate of adventitious shoots from callus was 69.56% when NAA(0.5 mg/L)and 6-BA(2.0 mg/L)were added in the MS medium. The MS medium containing 6-BA 3.0 mg/L and NAA 0.8 mg/L gave the better response to adventitious shoots induction from cotyledon explants, and the induction rate reached up to 72.33%. About 70.00 % of the adventitious shoots rooted on 1/2 MS containing 0.5 mg/L IAA.

Key words: Ligularia rumicifolia(Drumm.)S. W. Liu, callus, adventitious shoots, plant regeneration

CLC Number:

Q945

LI Yi, LU Xu, GUO Meng, LU Jiao, WANG Junli. Tissue culture of Ligularia rumicifolia[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(1): 55-59.

References

[1] 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1989.
[2] 西藏自治区卫生局、西藏军区后勤部卫生处.西藏常用中草药[M]. 西藏:西藏人民出版社,1973.
[3] 帝玛尔·丹增彭措著.晶珠本草[M].毛继祖,译.上海:上海科学技术出版社,2012.
[4] 中国药材公司.中国中药资源志要[M].北京:科学出版社,1994.
[5] 张一鸣,林昱星,张璐,等.奇异南星再生体系的建立[J].河北大学学报(自然科学版),2017,37(3):262-266. DOI:10.3969/j.issn.1000-1565.2017.03.007.
[6] WANG J L, WANG J, LIU K, et al. An efficient plant regeneration system with in vitro flavonoid accumulation for Hylotelephium tatarinowii(Maxim.)H. Ohba[J]. In Vitro Cell Dev Biol—Plant, 2010, 46(5): 445-450. DOI:10.1007/s11627-010-9315-6.
[7] WANG J L, LIU K, XU D T, et al. Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning[J]. Plant Growth Regul, 2013, 69(3):305-310. DOI:10.1007/s10725-012-9765-4.
[8] GUO B, GAO M, LIU C Z. In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir[J]. Plant Cell Rep, 2007, 26(3):261-265. DOI:10.1007/s00299-006-0230-6.
[9] GRABKOWSKA R, SITAREK P, WYSOKINSKA H. Influence of thidiazuron(TDZ)pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens[J]. Acta Physiol Plant, 2014, 36(7):1661-1672. DOI:10.1007/s11738-014-1541-9.
[10] SLAZAK B, SLIWINSKA E, SALUGA M, et al. Micropropagation of Viola uliginosa(Violaceae)for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis[J]. Plant Cell Tissue Organ Cult, 2015, 120(1):179-190. DOI:10.1007/s11240-014-0592-3.
[11] 杜娟,杨柏明,李彦舫.蹄叶橐吾的组织培养及植株再生[J].植物生理学通讯,2006,42(4):687.
[12] 高国超,黄楠,廖萍,等.蹄叶橐吾组织培养及快速繁殖技术研究[J].湖南农业科学,2011(11):27-29.DOI:10.16498/j.cnki.hnnykx.2011.11.031.
[13] 庄倩倩,陈少鹏,顾德峰,等.单花橐吾愈伤组织诱导及分化培养条件研究[J].中国草地学报,2013,35(3):24-30.
[14] 潘艳艳,那少众,王福维,等.全缘橐吾组培再生体系的研究[J].江苏农业科学,2012,40(1):56-59.DOI:10.15889/j.issn.1002-1302.2012.01.083.
[15] 朱丽,林夏珍.大头橐吾的组织培养与快速繁殖[J].植物生理学通讯,2009,45(10):1003-1004.DOI:10.13592/j.cnki.ppj.2009.10.023. (