Activiation of the inflammasomes in trophoblastic cells by Lisetria monocytogenes

doi:10.3969/j.issn.1000-1565.2022.01.010

Abstract

Abstract: To explore the role of Lisetria monocytogenes(LM)-induced mouse trophoblastic cell inflammasome activation in LM-induced mouse abortion, mouse trophoblastic cells were infected with LM. After the mouse trophoblastic cells were infected with LM in vitro,distribution of LM were observed. Interleukin(IL)-1β level in cell medium, cleavaged cysteinyl aspartate specific proteinase 1(caspase 1)level in the cell lysate and the amount of lactate dehydrogenase(LDH)release were detected. Results showed that LM distributed around the cell nucleus. Both IL-1β level in cell medium, cleavaged caspase 1 level in the cell lysate and the amount of LDH release were increased significantly in LM-infected group compared to control group, while the IL-1β level and the cleavaged caspase 1 level of the listeriolysin O(LLO)-deficient LM(Δ hly LM)-infected group were obviously lower than that of wild LM-infected group. Therefore, it is speculated that LM can activiate mouse trophoblastic inflammasome by LLO and promote the secretion of IL-1β and the pyrotosis of trophoblastic cells, which induced the mouse abortion. These results can provide a scientific basis for the clinical prevention and treatment on LM-induced abortion.

Key words: Listeria monocytogenes, mouse, abortion, trophoblastic cell, inflammasome

CLC Number:

S852.61

LI Wenyan, CHEN Yingying, DONG Ziyi, LIU Jie, XIAO Feng, ZHANG Yuhang. Activiation of the inflammasomes in trophoblastic cells by Lisetria monocytogenes[J]. Journal of Hebei University(Natural Science Edition), 2022, 42(1): 73-77.

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