Composition of the intestinal bacteria and their cellulase characterization in Cathaica fasciola

doi:10.3969/j.issn.1000-1565.2017.04.011蜗牛肠道细菌及其纤维素酶性质

Abstract

Abstract: In order to explore the functional microorganism, the intestinal culturable microflora in Cathaica fasciola were screened and cultured by several kinds of culture media, and the cellulase activity of the isolated bacteria and the characteristics of the crude cellulase that produced by strain S6 was studied.The results showed that the largest number of intestinal bacteria was up to 3.3×10⁴ CFU/mL in LB medium.Congo red staining showed that all the 7 dominant bacteria had the ability to secrete cellulase.The strain S6 had highest CMCase and FPase values of 5.96 and 2.84 U/mL, respectively.The CMCase characterization revealed that, the optimum reaction temperature was 35 ℃, the cellulose kept its high activity from 55 ℃to 75 ℃.The crude enzyme still had 60% of the highest obvious activation function at pH 8.0.Mg²⁺、Na⁺、K⁺and Ca²⁺ had enhancing effect, and Zn²⁺、Cu²⁺ and Mn²⁺ had inhibitory action.To research the composition of the snail intestinal bacteria and separate high-yield cellulase strains is a new way for the cellulose biodegradation into renewable energy.

Key words: Cathaica fasciola, intestinal bacteria, enzyme producing capabilities, cellulose biodegradation

CLC Number:

Q939.9

CHANG Yanping, TIAN Qifan, GUO Xin, LI Yanqin, YAN Leilei, LI Chunqing. Composition of the intestinal bacteria and their cellulase characterization in Cathaica fasciola[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(4): 400-404.

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References

[1] MONTGOMERY R.Development of biobased products[J].Bioresour Technol, 2004, 91(1): 1-29.DOI:10.1016/s0960-8524(03)00154-8.
[2] LO N, TOKUDA G, WATANABE H, et al.Evidence from multiple gene sequences indicates that termites evolved from wood-feeding cockroaches[J].Curr Biol, 2000, 10(13): 801-804.DOI:10.1016/s0960-9822(00)00561-3.
[3] DESVAUX M.The cellulosome of Clostridium cellulolyticum[J].Enzyme and Microbial Technology, 2005, 37(4): 373-385.DOI:10.1016/j.enzmictec.2004.04.025.
[4] FOLEY B.Heterotrophic nutrition in animals [A] / KNOX B,LADIGES P, EVANS B, et al. Biology(2nd)[C].Roseville Australia: McGraw-Hill,2001: 486-515.
[5] TIAN C, BEESON W T, IAVARONE A T, et al.Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa[J].Proceedings of the National Academy of Sciences, 2009, 106(52): 22157-22162.DOI:10.1073/pnas.0906810106.
[6] 田敏, 张庆华, 毛忠贵, 等.一株高温兼性纤维素降解细菌的分离鉴定及特性分析[J].安全与环境学报, 2013, 13(2): 31-36.DOI:10.3969/j.issn.1009-6094.2013.02.008. TIAN M, ZHANG Q H, MAO Z G, et al.Isolation and identification of a thermophilic cellulolytic facultative anaerobic bacterium and analysis of its properties[J].Journal of Safety and Environment, 2013, 13(2): 31-36.DOI:10.3969/j.issn.1009-6094.2013.02.008.
[7] 陈德牛,张国庆.中国动物志:无脊椎动物第3 7卷软体动物门、腹足纲、巴蜗牛科M].北京:科学出版社,2004:216-220.
[8] 张超, 李朝品, 刘群红.天螺多糖抑制HBV复制的体外实验研究[J].中国实验方剂学杂志, 2004, 10(4): 63-64.DOI:10.3969/j.issn.1005-9903.2004.04.026.
[9] GHOSH K, SEN S K, RAY A K.Characterization of bacilli isolated from the gut of rohu,Labeo rohita, fingerlings and its significance in digestion[J].Journal of Applied Aquaculture, 2002, 12(3): 33-42.DOI:10.1300/j028v12n03_04.

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