Effects of Rpf factor of Micrococcus luteus on the isolation of soil culturable species

doi:10.3969/j.issn.1000-1565.2019.01.011

Abstract

Abstract: Bacteria in three soil samples were isolated by adding Rpf factor from Micrococcus luteus to the medium to understand the effect of Rpf factor on the culturable species diversity. By adding the Rpf factor of M. luteus to the modified VL55 medium, the bacteria were isolated from three soil samples collected from Shanggang Village, Laishui County, Hebei Province, Sanjiangyuan, Qinghai, and Wuliangshan, Yunnan. The phylogenetic analysis of the 16S rRNA gene sequence was carried out on the isolated- DOI:10.3969/j.issn.1000-1565.2019.01.011藤黄微球菌Rpf因子对土壤细菌可培养物种分离效果的影响李云琪,王宇辉,李小锦,孙健鹏,景凤霞,张秀敏(河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北省微生物育种与保育工程实验室,河北保定 071002)摘要:通过培养基添加藤黄微球菌Rpf因子对3份土壤样品中的细菌进行分离以了解藤黄微球菌Rpf因子对细菌可培养物种多样性的影响.通过在改良的VL55培养基中添加藤黄微球菌Rpf因子,对采自河北涞水县上港村、青海三江源和云南无量山3个地区的3份土壤样品进行细菌的分离,并对所分离菌株进行16S rRNA基因序列系统发育分析,分析培养基添加藤黄微球菌Rpf因子对土壤中可培养细菌物种分离效果的影响.结果显示:河北土壤中不添加Rpf因子的对照组分离得到5个属,添加Rpf因子的实验组分离得到8个属;青海土壤中对照组分离得到2个属,实验组分离得到6个属;云南土壤中对照组分离得到6个属,实验组分离得到8个属.表明添加藤黄微球菌Rpf因子的实验组分离得到的微生物物种较对照组丰富. 其中,青海三江源土壤中分离得到的菌株HBUM200161与多食鞘氨醇杆菌(Sphingobacterium multivorum)IAM14316^T亲缘关系最近,16S rRNA基因序列相似性为97.93%,可能为鞘氨醇杆菌属(Sphingobacterium)潜在的新种.关键词:藤黄微球菌;Rpf;细菌;分离中图分类号:Q393.13 文献标志码:A 文章编号:1000-1565(2019)01-0063-06Effects of Rpf factor of Micrococcus luteus on the isolation of soil culturable speciesLI Yunqi, WANG Yuhui, LI Xiaojin, SUN Jianpeng, JING Fengxia, ZHANG Xiumin ( Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province,Key Laboratory of Microbial Diversity Research and Application of Hebei Province,College of Life Sciences, Hebei University, Baoding 071002, China )Abstract:Bacteria in three soil samples were isolated by adding Rpf factor from Micrococcus luteus to the medium to understand the effect of Rpf factor on the culturable species diversity. By adding the Rpf factor of M. luteus to the modified VL55 medium, the bacteria were isolated from three soil samples collected from Shanggang Village, Laishui County, Hebei Province, Sanjiangyuan, Qinghai, and Wuliangshan, Yunnan. The phylogenetic analysis of the 16S rRNA gene sequence was carried out on the isolated- 收稿日期:2018-05-25 基金项目:国家自然科学基金资助项目( 31270053 );河北省自然科学基金资助项目(C2014201141) 第一作者: 李云琪(1993—),女,河北保定人,河北大学在读硕士研究生.E-mail:1139539385@qq.com 通信作者:张秀敏(1970—),女,河北涞水人,河北大学教授,博士,主要从事微生物系统性及多样性研究. E-mail:zhxiumin1106@126.com第1期李云琪等:藤黄微球菌Rpf因子对土壤细菌可培养物种分离效果的影响strains, and the effect of adding Rpf factor to the culture medium on the separation of culturable bacterial species in the soil was analyzed. The results showed that five genera were isolated from the control group in Hebei soil without Rpf factor, and eight genera were isolated from the test group with Rpf factor; two genera were isolated from the control group in Qinghai soil, and six genera were isolated from the test group; in the soil of Yunnan, six genera were isolated from the control group, and eight genera were isolated from the test group. The results show that the microbial species isolated from the test group with M. luteus Rpf added was more abundant than that of the control group. Among them, the strain HBUM200161 isolated from the Sanjiangyuan soil in Qinghai has the closest relationship with Sphingobacterium multivorum IAM14316^T, and the 16S rRNA gene sequence similarity is 97.93%, the strain probably represents a novel species of the genus Sphingobacterium.

Key words: Micrococcus luteus, Rpf, bacteria, isolated

CLC Number:

Q393.13

LI Yunqi, WANG Yuhui, LI Xiaojin, SUN Jianpeng, JING Fengxia, ZHANG Xiumin. Effects of Rpf factor of Micrococcus luteus on the isolation of soil culturable species[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(1): 63-68.

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