一种荧光增强型的GSH荧光探针

doi:10.3969/j.issn.1000-1565.2019.01.009

河北大学学报(自然科学版) ›› 2019, Vol. 39 ›› Issue (1): 49-55.DOI: 10.3969/j.issn.1000-1565.2019.01.009

一种荧光增强型的GSH荧光探针

孟美荣¹,阴彩霞²

收稿日期:2018-10-20 出版日期:2019-01-25 发布日期:2019-01-25
通讯作者: 阴彩霞(1978—),女,山西平遥人,山西大学教授,主要从事有机小分子荧光探针的研究. E-mail:yincx@sxu.edu.cn
作者简介:孟美荣(1982—),女,山西朔州人,山西大学讲师,主要从事荧光探针的识别研究.
基金资助:
国家自然科学基金资助项目(21672131;21775096;21705102);山西省人才支持计划资助项目(2014401);山西省归国基金资助项目(2017-026)

A turn-on fluorescent probe for GSH

MENG Meirong¹, YIN Caixia²

1.School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China; 2. Institute of Molecular Science, Shanxi University, Taiyuan 030006, China

Received:2018-10-20 Online:2019-01-25 Published:2019-01-25

摘要/Abstract

摘要： 常见的生物硫醇包括半胱氨酸(Cys)、同型半胱氨酸(Hcy)以及谷胱甘肽(GSH),它们在人体中起着十分重要的作用.使用荧光探针检测此类生物硫醇具有灵敏度高、选择性好、响应时间快等优势.由于3种硫醇具有相似的化学结构(含有活性巯基),因此给这类生物硫醇的选择性检测带来挑战.本文设计了一种荧光探针(2-甲基,6-丙烯酰基喹啉)用于区分检测GSH和Cys/Hcy.通过测试该探针的光谱性质,发现在含有该探针的水溶液中加入谷胱甘肽(GSH)后,相应的荧光光谱和紫外-可见光谱都有显著的变化.相比其他分析物,发现探针在水溶液中对GSH具有较高的选择性和灵敏度.此外,考虑到该检测过程是在水相中进行的,因此该探针在生物成像方面具备潜在的使用价值.

关键词: 荧光探针, 喹啉, 生物硫醇, 荧光增强

Abstract: As we all known that homocysteine(Hcy), cysteine(Cys), and glutathione(GSH)are common biothiols which play a crucial role in the human body. The use of fluorescent probes for detection of biothiols has many advantages, such as high sensitivity, good selectivity, and short response times. Because of the similar chemical structure(containing active sulfhydryl groups)of these biothiols, the selective detection of them is a challenge. In this article, we designed a GSH fluorescent probe(2-methyl, 6-acryloyl quinoline)to distinguish Cys/Hcy. By testing the spectral properties of the probe, we found that the addition of GSH in aqueous solution containing the probe caused a significant change in the fluorescence and UV-visible spectra. After comparing with other analytes, we found that the probe had higher selectivity and sensitivity to GSH in H₂O. In addition, considering that the detection process is performed in the aqueous phase, so the probe has a potential application value in biological imaging.

Key words: fluorescent probe, quinoline, biothiol, fluorescence enhancement

中图分类号:

O316

孟美荣,阴彩霞. 一种荧光增强型的GSH荧光探针[J]. 河北大学学报(自然科学版), 2019, 39(1): 49-55.

MENG Meirong, YIN Caixia. A turn-on fluorescent probe for GSH[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(1): 49-55.

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一种荧光增强型的GSH荧光探针

A turn-on fluorescent probe for GSH

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