Quality standard of Qihuang Xingnao Granules

doi:10.3969/j.issn.1000-1565.2024.04.005

Abstract

Abstract: The quality standard of Qihuang Xingnao Granule is established to provide reference for its quality control. Thin layer chromatography(TLC)was used to identify Barbary wolfberry, Epimedium, Salvia miltiorrhiza, Rhizoma curculiginis, Angelica sinensis and Polygonum multiflorum in the granules. The content of diphenylvinyl glycosides in the preparation was determined by high performance liquid chromatography(HPLC). The HPLC conditions were: Waters Bridge C₁₈ column(4.6× 250 nm, 5 μL), methanol-water(36∶64), detection wavelength of 320 nm, flow rate of 1.0 mL/min, and a column temperature of 30 ℃. The main TLC spots of Barbary wolfberry, Epimedium, Salvia miltiorrhiza, Rhizoma curculiginis, Angelica sinensis and Polygonum multiflorum were clear, the separation was good, and the negative control had no interference. The stilbenside was linearly related in the range of 0.195 6 to 2.934 0 μg(R²=0.999 5, n=6), the RSD of precision, repeatability and stability was less than 2.0%, the sample recovery rate ranged from 96.38% to 100.19%, and the RSD was 1.48%. The quality standard project is set reasonably, convenient and accurate, and can be used for the quality control of Qihuang Xingnao Granules.

Key words: Qihuang Xingnao Granules, quality standard, diphenylvinyl glycosides, TLC, HPLC

CLC Number:

R927
R917

SHAO Zhenzhen, ZHAO Ziwei, LIU Xiaomeng, LI Lili, ZHANG Yali, HA Jing. Quality standard of Qihuang Xingnao Granules[J]. Journal of Hebei University(Natural Science Edition), 2024, 44(4): 373-381.

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