Callus proliferation and accumulation of tea polyphenols of Anji Baicha

doi:10.3969/j.issn.1000-1565.2017.06.009

Abstract

Abstract: For utilization of Anji Baicha,the effects of different plant growth regulators on callus,proliferation and accumulation of tea polyphenols of Anji Baicha were investigated using leaf as explants.The results showed that TDZ was more effective for callus induction,proliferation and accumulation of tea polyphenols.The callus induction frequency reached 92.1 % when the concentration of TDZ was as high as 1.0 mg/L.The mass fraction of tea polyphenols was 20.6%,and the relative abundance of epicatechin increased to 5.3%.

Key words: Anji Baicha, callus proliferation, tea polyphenols, GC-MS analyses

CLC Number:

Q945

LIN Yuxing,XIAO Zefeng,TIAN Birui,HAO Na,WANG Junli. Callus proliferation and accumulation of tea polyphenols of Anji Baicha[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(6): 614-620.

References

[1] 郭雅敏.安吉白茶的性状与发展前景[J].茶叶,1997(4):23-24. GUO Y M.Characteristics and development prospects of Anji Baicha [J].Journal of Tea,1997(4):23-24.
[2] LI Q,HUANG J A,LIU S Q,et al.Proteomic analysis of young leaves at three developmental stages in albino tea cultivar [J].Proteome Science,2011,9(1):44.DOI: 10.1186/1477-5956-9-44.
[3] 陆文渊,钱文春,赖建红,等.安吉白茶产业的现状及发展对策[J].茶叶科学技术,2012(1):25-27. LU W Y,QIAN W C,LAI J H,et al.Development status and its countermeasures of Anji Baicha[J].Tea Science and Technology,2012(1):25-27.
[4] 于淑池,刘畅,王乘慧,等.安吉白茶茶多酚的抗氧化活性研究[J].时珍国医国药,2012,23(5):1184-1187.DOI:10.3696/j.issn.1008.0805.2012.05.062. YU S C,LIU C,WANG C H,et al.Antioxidant activity of tea polyphenols of Anji Baicha[J].Lishizhen Medicine and Materia Medica Research,2012,23(5):1184-1187.DOI:10.3696/j.issn.1008.0805.2012.05.062.
[5] 吕娜,张伟臻,陈林龙,等.安吉白茶黄酮抗氧化活性的体外实验研究[J].毒理学杂志,2014,28(5):389-392.DOI: 10.16421/j.cnki.1002-3127.2014.05.008. LÜ N,ZHANG W Z,CHEN L L,et al.In vitro experimental study on antioxidant activity of flavonoids of Anji Baicha [J].Journal of Toxicology,2014,28(5):389-392. DOI: 10.16421/j.cnki.1002-3127.2014.05.008.
[6] 夏道宗,张元君,倪达美,等.安吉白茶多糖抗肿瘤及免疫调节研究[J].茶叶科学,2013,33(1):40-44.DOI:10.13305/j.cnki.jts.2013.01.008. XIA D Z,ZHANG Y J,NI D M,et al.Studies on antitumor and immune regulation of polysaccharide of Anji Baicha[J].Tea Science and Technology,2013,33(1):40-44.DOI:10.13305/j.cnki.jts.2013.01.008.
[7] HAJIAGHAALIPOUR F,KANTHIMATHI M S,SANUSI J,et al.White tea(Camellia sinensis)inhibits proliferation of the colon cancer cell line,HT-29,activates caspases and protects DNA of normal cells against oxidatiive damage[J].Food Chemistry,2015,169: 401-410. DOI: 10.1016/j.foodchem.2014.07.005.
[8] 吕娜,韦薇,顾帅,等.安吉白茶对柱状黄杆菌抑菌机理的研究[J].中国畜牧兽医,2015,42(1):136-139.DOI:10.16431/j.cnki.1671-7236.2015.01.021. LÜ N,WEI W,GU S,et al.Study on bacteriostatic mechanism of flavobacterium columnare of Anji Baicha[J].China Animal Husbandry & Veterinary Medicine,2015,42(1):136-139. DOI:10.16431/j.cnki.1671-7236.2015.01.021.
[9] 薛寒青,王舰,周淑兰.TDZ对诱导大蒜愈伤组织形成的影响[J].长江蔬菜,2009(14):19-20.DOI:10.3865/j.issn.1001-3547.2009.14.006. XUE H Q,WANG J,ZHOU S L.Effect of TDZ on callus induction of garlic [J].Journal of Changjiang Vegetables,2009(14):19-20.DOI:10.3865/j.issn.1001-3547.2009.14.006.
[10] 康大力,张洪利,莫小路,等.TDZ对喜树愈伤组织生长及色素积累的影响[J].生物技术,2012,22(1):83-85. DOI: 10.3696/j.issn.1004-311x.2012.01.023. KANG D L,ZHANG H L,MO X L,et al.Effect of TDZ on callus of growth and pigment accumulation of Camptotheca acuminata[J].Biotechnology,2012,22(1):83-85. DOI:10.3696/j.issn.1004-311x.2012.01.023.
[11] WANG J L,XIAO X,WANG Q,et al.Accumulation of flavonoids and antioxidant activity of Stellera chamaejasme by an efficient callus culture[J].Hort Environ Biotechhnol,2013,54(5):441-449.DOI 10.1007/s13580-013-0171-2.
[12] 杜鸿标,王坤波,陈勤操,等.茶树愈伤组织诱导培养及儿茶素合成的研究[J].茶叶通讯,2014,41(1):11-14. DU H B,WANG K B,CHEN Q C,et al.Study on callus induction and catechin synthesis of tea [J]. Tea Communication,2014,41(1):11-14.

[1]	HUANG Hangjun, LI Yi, MA Linxi, MA Weiwei, WANG Junli. Changes of antioxidant enzyme activity in callus of Hylotelephium tatarinowii under PEG 6 000 stress [J]. Journal of Hebei University (Natural Science Edition), 2020, 40(4): 415-420.
[2]	LIANG Yuling,XIANYU Liangyan,LIU Wenwen,ZHANG Yuanyuan. Direct shoot regeneration in vitro for Atractylodes macrocephala using epicotyl and radicle as explants [J]. Journal of Hebei University (Natural Science Edition), 2020, 40(2): 171-175.
[3]	MA Weiwei, HUANG Hangjun, BI Kaili, LI Yi, WANG Junli. Determination of phenols and antioxidant activity of inflorescence from Scabiosa tschiliensis Grunning [J]. Journal of Hebei University (Natural Science Edition), 2019, 39(6): 637-642.
[4]	LIU Mingfei, MA Weiwei, HUANG Jingjing, WANG Zhongyue, WANG Junli. Antifungal activity of Nepeta cataria L. against 4 pathogenic fungi isolated from grape [J]. Journal of Hebei University (Natural Science Edition), 2019, 39(1): 69-75.
[5]	HAO Na, MA Weiwei, HUANG Hangjun, LI Jianfei, WANG Junli. Chemical constituents and antioxidant activity of Dracocephalum rupestre Hance [J]. Journal of Hebei University (Natural Science Edition), 2018, 38(6): 617-622.
[6]	ZHANG Yiming,LIN Yuxing,ZHANG Lu,XIAO Zefeng,WANG Junli. Establishment of regeneration system of Arisaema decipiens Schott [J]. Journal of Hebei University (Natural Science Edition), 2017, 37(3): 262-266.
[7]	LI Si,ZHANG Li,YAO Yaqin. Effects of different water stress on active oxygen, stoma and photosynthesis characteristics of wheat [J]. Journal of Hebei University (Natural Science Edition), 2015, 35(5): 487-493.
[8]	GUO Meng,XU Dongting,WANG Junli,ZHANG Rongrong,HUANG Jingjing. Establishment of regeneration system and flavonoids analysis of Scabiosa tschiliensis Grunning [J]. Journal of Hebei University (Natural Science Edition), 2015, 35(4): 379-384.
[9]	LI Jianfei,WANG Junli,GONG Weizhen,FEI Liangdan,LIU Jindou. Antioxidation, antibacterial and antitumor activities of Anemone cathayensis [J]. Journal of Hebei University (Natural Science Edition), 2014, 34(4): 414-420.
[10]	YU Xiumin,YUE Wenran,WANG Ruigang. Cloning and expression study of CMV promoter in plant [J]. Journal of Hebei University (Natural Science Edition), 2014, 34(2): 187-192.
[11]	ZHANG Lu,WANG Junli,WANG Jue,TIAN Birui,MA Linxi. Plant tissue culture and establishment of regeneration system of Hylotelephium tatarinowii (Maxim.) H.Ohba [J]. Journal of Hebei University (Natural Science Edition), 2013, 33(6): 648-653.
[12]	SONG Yunfei,WANG Junli,BI Kaili,ZHANG Lu,LI Jianfei. Tissue culture and plant regeneration of Primula maximowiczii [J]. Journal of Hebei University (Natural Science Edition), 2012, 32(6): 630-634.
[13]	LIANG Yu-ling,LEI Qi-yao. Regulation on growth and glycyrrhiza polysaccharide biosynthesis of Glycyrrhiza in flata callus [J]. Journal of Hebei University (Natural Science Edition), 2012, 32(3): 299-303.
[14]	ZHANG Zhuo,WANG Yan,WANG Xiao-xu,LI Hui,JIANG Chang-yang. Clone construction through direct differentiation from tender stems of Ixeris chinensis [J]. Journal of Hebei University (Natural Science Edition), 2012, 32(2): 180-186.
[15]	JIANG Ji-zhi,GUO Jun-ting,LI Li-yan. Advances of antagonistic fungi against Phytophthora infestans [J]. Journal of Hebei University (Natural Science Edition), 2012, 32(1): 105-112.