Analysis and determination of the main components in the four medicinal materials of Yinzhihuang formula by HPLC

doi:10.3969/j.issn.1000-1565.2020.05.008

Abstract

Abstract: The chemical constituents of the standard extracts of the four medicinal materials in Yinzhihuang formula were analyzed by HPLC, and determined the content of chlorogenic acid, 1,3-dicaffeoylquinic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, geniposide, baicalin, wogonin, baicalein, wogonoside, oroxylin A, and neochlorogenic acid. The separation was carried out on a Diamonsil C₁₈(250 mm×4.6 mm,5 μm)column, and gradient eluted with the mobile phase of acetonitrile(A)-0.1% formic acid aqueous solution(B)with 1.0 mL/min. The column temperature was set at 30 ℃. The main components in the four standard medicinal extracts of Yinzhihuang formula can be separated in the chromatographic conditions. Precision, repeatability, and stability were good, and the average recoveries were between 98.84%~102.22%. The developed method was simple and effective for the determination of the components in the four medicinal materials, and the result is accurate and reliable, it can provide a reference for the Yinzhihuang formulation of the quality standard of medicinal extracts and a basis for the analysis of the main chemical components.

Key words: Yinzhihuang formula, HPLC, component analysis, content determination

CLC Number:

R917

ZHANG Yushan, SUN Yifei, LUO Li, LI Zuoming, ZHANG Shanzi, YAO Xincheng. Analysis and determination of the main components in the four medicinal materials of Yinzhihuang formula by HPLC[J]. Journal of Hebei University (Natural Science Edition), 2020, 40(5): 500-509.

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