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25 May 2023, Volume 44 Issue 3

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Complex force pattern and mechanical simulation analysis of Block 0 of the main pier of continuous variable section box girder

YANG Sanqiang, ZHANG Dan, HUANG Jingyong, DAI Zeyu, CAO Yawen

2024, 44(3):  225-234.  DOI: 10.3969/j.issn.1000-1565.2024.03.001

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A continuous variable section box girder is the widely used type of steel box girder bridge, which has the characteristics of complex force and difficult construction deformation control. This paper relies on the project of Baigou River Special Bridge on the Jing-Xiong Expressway Gaobeidian link of the backbone road network of Xiongan New Area, in combination with steel box girder structure of the anisotropy structure design theory, the linear monitoring, millimeter wave radar monitoring, stress-strain gauge monitoring and numerical simulation analysis method, toobtain the stress and deformation data of Block 0 of the main pier of continuous variable section box girder, The working mechanism of complex stress mode of Block 0 of the main pier of continuous variable section box girder is obtained. The results- DOI:10.3969/j.issn.1000-1565.2024.03.001连续变截面箱梁主墩0号块复杂受力模式与力学仿真分析杨三强¹,张丹¹,黄井勇²,代泽煜¹,曹亚文¹(1.河北大学 建筑工程学院,河北省土木工程监测与性能评估技术创新中心,河北 保定 071002;2.汇通建设集团股份有限公司,河北 高碑店 074000)摘 要:连续变截面箱梁是钢箱梁桥中应用较多的型式,具有受力复杂、施工变形控制难的特点.依托雄安新区骨干路网京雄高速高碑店连接线白沟河特大桥项目,结合钢箱梁结构异性结构设计理论,采用线性监测、毫米波雷达监测、埋设应变片应力监测与数值仿真分析相结合的方法,获得连续变截面箱梁主墩0号块各项受力变形数据,得到了连续变截面箱梁主墩0号块复杂受力模式的工作机制.结果表明: 1)针对线性监测、内应力监测与毫米波雷达监测,提出了连续变截面箱梁主墩0号块控制阀值范围,内应力变化范围为0.71~12.58 MPa,沉降量0.78~-0.2 mm;2)对比分析线性监测与内应力监测数据,并对其进行拟合回归,构建了主墩0号块变形受力模型;3)通过提取主墩0号块毫米波雷达波谱图,获取其变形异常点数据,分析得出了连续变截面箱梁主墩0号块失稳破坏工作机制.上述研究成果可为连续变截面箱梁主墩0号块设计参数优化、主墩施工质量控制与施工工艺优化提供理论支撑与技术指导.关键词:变截面箱梁;0号块;受力模式;力学仿真中图分类号:U448.21 文献标志码:A 文章编号:1000-1565(2024)03-0225-10Complex force pattern and mechanical simulation analysis of Block 0 of the main pier of continuous variable section box girderYANG Sanqiang¹, ZHANG Dan¹, HUANG Jingyong², DAI Zeyu¹, CAO Yawen¹(1. Hebei Province Civil Engineering Monitoring and Performance Evaluation Technology Innovation Center, College of civil Engineering and Architecture, Hebei University, Baoding 071002,China;2. Huitong Construction Group Co., Ltd., Gaobeidian 074000,China)Abstract: A continuous variable section box girder is the widely used type of steel box girder bridge, which has the characteristics of complex force and difficult construction deformation control. This paper relies on the project of Baigou River Special Bridge on the Jing-Xiong Expressway Gaobeidian link of the backbone road network of Xiongan New Area, in combination with steel box girder structure of the anisotropy structure design theory, the linear monitoring, millimeter wave radar monitoring, stress-strain gauge monitoring and numerical simulation analysis method, toobtain the stress and deformation data of Block 0 of the main pier of continuous variable section box girder, The working mechanism of complex stress mode of Block 0 of the main pier of continuous variable section box girder is obtained. The results- 收稿日期:2022-08-05;修回日期:2023-09-22 基金项目:保定市科技支撑计划项目(2011ZG010);河北省交通运输厅科技项目(PHP-C34200-2503631-1) 第一作者:杨三强(1980-),男,河北大学教授,主要从事桥梁监控、公路路基和路面材料的研究.E-mail:ysq0999@163.com 通信作者:张丹(1997-),女,河北大学在读硕士研究生,主要从事桥梁监控、公路路基和路面材料的研究.E-mail:zd971218@163.com第3期杨三强等:连续变截面箱梁主墩0号块复杂受力模式与力学仿真分析show that: 1)for linear monitoring, internal stress monitoring, and millimeter wave radar monitoring, the control threshold range of zero blocks of the main pier of continuous variable section box girder was proposed, the internal stress variation range is 0.71~12.58 MPa, and the settlement amount is 0.78~-0.2 mm; 2)the data of linear monitoring and internal stress monitoring were compared and analyzed, and the fitting regression was carried out to construct the deformation stress model of the main pier blocks; 3)by extracting the millimeter wave radar spectrum of the main pier zero block and obtaining data on abnormal deformation points, the instability failure mechanism of the main pier zero blocks of continuous variable section box girder was analyzed. The above research results can provide theoretical support and technical guidance for optimization of design parameters, construction quality control, and construction process optimization of Block 0 of the main pier of contionuous variable section box girder.

Sulfate resistance of high performance concrete with non-spontaneous combustion coal gangue coarse aggregate

WANG Zhigang, LI Ying, LIU Mingze, GAO Qixiang, NIU Xiaoyan, MA Hongqiang

2024, 44(3):  235-246.  DOI: 10.3969/j.issn.1000-1565.2024.03.002

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In order to expand the application range of non-spontaneous combustion coal gangue(NCCG)concrete and reduce the environmental pollution caused by coal gangue stacking, NCCG is substituted for natural gravel to prepare green high-performance concrete. The effects of different water-binder ratio, coarse aggregate substitution rate, mineral admixture type and dosage, water-cement ratio of wolfing slurry on its sulfate attack resistance were systematically studied. The loss mechanism of wolfing slurry was investigated by SEM-EDS and XRD. The results showed that the higher the water-binder ratio is, the higher the damage index is. The best sulfate resistance was obtained by mixing 20% slag powder and 10% mass fraction fly ash. Silica fume coated NCCG aggregate has no obvious improvement in sulfate- DOI:10.3969/j.issn.1000-1565.2024.03.002非自燃煤矸石粗骨料高性能混凝土耐硫酸盐侵蚀性能王志刚¹,李颖²,刘明泽²,高琦翔²,牛晓燕²,马宏强²(1. 广东石油化工学院 建筑工程学院,广东 茂名 525000;2. 河北大学 建筑工程学院,河北 保定 071002)摘 要:为扩大非自燃煤矸石(NCCG)混凝土应用范围,减少煤矸石堆放对环境造成的污染,将NCCG代替天然碎石制备绿色高性能混凝土,系统地研究不同水胶比、粗骨料取代率、矿物掺合料种类与掺量、硅灰裹浆水灰比对其耐硫酸盐侵蚀性能的影响,并借助扫描电镜-能谱分析(SEM-EDS)与X线衍射(XRD)探讨损失机理.结果表明:水胶比越大,各项损伤指标越大;复掺质量分数20%矿渣粉与质量分数10%粉煤灰时耐硫酸盐侵蚀性能最佳;硅灰裹浆NCCG骨料对提高耐硫酸盐侵蚀性能不明显.NCCG取代率越大、水胶比越大生成的钙矾石和石膏等侵蚀产物越多,复掺粉煤灰与矿渣粉时生成的侵蚀产物最少,建议工程中NCCG取代率不宜超过30%,按照矿渣粉与粉煤灰2∶1的质量比复掺使用2种矿物掺合料.关键词:非自燃煤矸石;高性能混凝土;硫酸盐侵蚀;损伤机理中图分类号:TU528 文献标志码:A 文章编号:1000-1565(2024)03-0235-12Sulfate resistance of high performance concrete with non-spontaneous combustion coal gangue coarse aggregateWANG Zhigang¹, LI Ying², LIU Mingze², GAO Qixiang², NIU Xiaoyan², MA Hongqiang²(1. College of Civil Engineering and Architecture, Guangdong University of Petrochemical Technology, Maoming 525000, China;2. College of Civil Engineering and Architecture, Hebei University, Baoding 071002, China)Abstract: In order to expand the application range of non-spontaneous combustion coal gangue(NCCG)concrete and reduce the environmental pollution caused by coal gangue stacking, NCCG is substituted for natural gravel to prepare green high-performance concrete. The effects of different water-binder ratio, coarse aggregate substitution rate, mineral admixture type and dosage, water-cement ratio of wolfing slurry on its sulfate attack resistance were systematically studied. The loss mechanism of wolfing slurry was investigated by SEM-EDS and XRD. The results showed that the higher the water-binder ratio is, the higher the damage index is. The best sulfate resistance was obtained by mixing 20% slag powder and 10% mass fraction fly ash. Silica fume coated NCCG aggregate has no obvious improvement in sulfate- 收稿日期:2023-06-12;修回日期:2023-10-11 基金项目:河北省自然科学基金资助项目(E2022201011);河北省高等学校科学研究项目(QN2022067) 第一作者:王志刚(1982—),男,广东石油化工学院高级实验师,主要从事土木工程材料方向的研究.E-mail:alexsea@gdupt.edu.cn 通信作者:牛晓燕(1979—),女,河北大学教授.主要从事工程材料理论与应用方向研究.E-mail: niu-xiaoyan2002@163.com第3期王志刚等:非自燃煤矸石粗骨料高性能混凝土耐硫酸盐侵蚀性能resistance. The larger the NCCG substitution rate is, the larger the water-binder ratio is, the more ettringite and gypsum will be generated, and the least erosion products will be generated when fly ash and slag powder are mixed and it is suggested that the NCCG substitution rate should not exceed 30% in the project, and two mineral admixtures should be used in a 2∶1 ratio of slag powder and fly ash.

Effect of dielectric constant of tube on characteristics of atmospheric pressure Ar cold plasma jets

YAO Tingyu, WANG Shuo, TIAN Miao, LIU Fucheng, HE Yafeng

2024, 44(3):  247-253.  DOI: 10.3969/j.issn.1000-1565.2024.03.003

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The effect of dielectric constant of tube on characteristics of atmospheric pressure Ar cold plasma jets has been investigated by using two-dimensional fluid model. It is shown that there are different features of plasma jets in and out of the tube. In the dielectric tube, the ionization wave accelerates firstly and then decelerates as it is propagating along with the dielectric surface, giving rise to the ring structure. The larger the dielectric constant, the smaller jet radius of the ring structure, the lower the electron density, and the smaller jet propagation velocity. When the plasma jet leaves the dielectric tube, its velocity increases dramatically and then slows down. The larger the dielectric constant is, the larger the acceleration is. The ring structure shrinks all the time and vanishes eventually in the jet propagating process. These results provide certain guiding significance for design and optimization of cold plasma sources.

Preparation, characterization and antigenicity of soybean 7S globulin-jujube polysaccharide complexes

ZHANG Chenjing, ZHANG Xirui, GUO Ziyao, HAN Dandan, DOU Haiyang

2024, 44(3):  254-260.  DOI: 10.3969/j.issn.1000-1565.2024.03.004

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In order to reduce the antigenicity of soybean, the soybean 7S globulin-Jujube polysaccharide(7S-JP)complexes were prepared by glycosylation reaction. The effects of reaction temperature, reaction time, and material ratio of 7S to JP on the structure of 7S-JP complexes were studied by asymmetrical flow field-flow fractionation coupled with online ultraviolet-visible detector, multi-angle light scattering detector, and differential refractive index detector(AF4-UV-MALS-dRI). Meanwhile, the radius of gyration, molar mass, and conformation of 7S-JP complexes were characterized by AF4-UV-MALS-dRI. The antigenicity of 7S-JP complexes was studied by indirect enzyme-linked immunosorbent assay. The results showed that: The 7S reacted with JP sufficiently at 70 ℃ in 20 min; The structure- DOI:10.3969/j.issn.1000-1565.2024.03.004大豆7S球蛋白-大枣多糖复合物的制备、表征及抗原性张晨婧¹,张西瑞²,郭子瑶¹,韩丹丹²,窦海洋¹(1.河北大学 基础医学院,河北省炎性自身免疫性疾病发病机制及防治重点实验室,河北 保定 071000;2.河北大学 公共卫生学院,河北 保定 071000)摘 要:为了降低大豆的抗原性,通过糖基化反应制备了大豆7S球蛋白-大枣多糖(7S-JP)复合物.采用非对称场流分离技术在线联用紫外可见光检测器、多角度光散射检测器和示差折光检测器(AF4-UV-MALS-dRI)研究糖基化反应温度、时间和物料比对7S-JP复合物结构的影响,对7S-JP复合物的回转半径、摩尔质量和构象进行表征;采用间接竞争酶联免疫吸附法探究7S-JP复合物的抗原性.AF4-UV-MALS-dRI结果表明:在反应温度为70 ℃、反应时间为20 min时,7S与JP糖基化反应效果最佳;当7S与JP的质量比为1∶3时,形成的7S-JP复合物结构紧密,有利于破坏其空间抗原表位,抗原抑制率为(69.1±0.99)%.关键词:大豆7S球蛋白;大枣多糖;非对称场流分离中图分类号:O657 文献标志码:A 文章编号:1000-1565(2024)03-0254-07Preparation, characterization and antigenicity of soybean 7S globulin-jujube polysaccharide complexesZHANG Chenjing¹, ZHANG Xirui², GUO Ziyao¹, HAN Dandan², DOU Haiyang¹(1. Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding 071000, China;2. College of Public Health, Hebei University, Baoding 071000, China)Abstract: In order to reduce the antigenicity of soybean, the soybean 7S globulin-Jujube polysaccharide(7S-JP)complexes were prepared by glycosylation reaction. The effects of reaction temperature, reaction time, and material ratio of 7S to JP on the structure of 7S-JP complexes were studied by asymmetrical flow field-flow fractionation coupled with online ultraviolet-visible detector, multi-angle light scattering detector, and differential refractive index detector(AF4-UV-MALS-dRI). Meanwhile, the radius of gyration, molar mass, and conformation of 7S-JP complexes were characterized by AF4-UV-MALS-dRI. The antigenicity of 7S-JP complexes was studied by indirect enzyme-linked immunosorbent assay. The results showed that: The 7S reacted with JP sufficiently at 70 ℃ in 20 min; The structure- 收稿日期:2024-01-24;修回日期:2024-03-12 基金项目:河北省重点研发项目(22372505D);河北省大学生创新创业训练计划项目(S202310075055);河北大学实验室开放项目(sy202245) 第一作者:张晨婧(2000—),女,河北大学在读硕士研究生,主要从事场流分离研究. E-mail: zhangccc10@163.com 通信作者:窦海洋(1983—),男,河北大学副教授,博士,主要从事场流分离研究.E-mail: douhaiyang-1984@163.com韩丹丹(1981—),女,河北大学教授,博士,主要从事卫生检验研究. E-mail: hdd@hbu.edu.cn第3期张晨婧等:大豆7S球蛋白-大枣多糖复合物的制备、表征及抗原性of 7S-JP complexes obtained with a material ratio of 1∶3 was more compact, which is beneficial to destroy its spatial epitope, and the antigen inhibition rate of 7S-JP(1∶3)achieved to be(69.1±0.99)%.

Preparation and characterization of Fe and Zn co-doped polyaniline microbial fuel cell cathode catalysts

CHAI Yuanji, WANG Hanming, LIU Zhimin,XIAO Longlong, SHEN Zhexi, YU Boqu

2024, 44(3):  261-268.  DOI: 10.3969/j.issn.1000-1565.2024.03.005

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In order to address the issues of low durability, slow kinetics of the oxygen reduction reaction(ORR), and high cost of cathode catalysts for microbial fuel cells(MFC), a porous carbon co-doped with Fe, Zn, and N as an air cathode catalyst was prepared. The electrochemical studies demonstrated that the synthesised catalyst Fe/Zn-NC-0.9 exhibited excellent electrocatalytic activity for the oxygen reduction reaction. The charge transfer resistance and the exchange current density were measured at 5.6 Ω and 53.3 mV/dec, respectively, while the maximum power density was at(1 253±20)mW/m². Additionally, the impact of the Fe doping level on the ORR reaction and maximum power density was assessed. It was found that a moderate Fe doping level is essential for enhancing catalytic activity. Furthermore, the cathode performance is improved through the synergistic effect of Fe and N co-doping.

Adsorption of tungsten by N-doped lignin

ZHANG Baoping, KE Jing, FANG Shiyuan, LI Wencan

2024, 44(3):  269-280.  DOI: 10.3969/j.issn.1000-1565.2024.03.006

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N-doped lignin used for adsorption of tungsten was synthesized by polyamination and quaternization from lignin. The adsorbent was characterized by SEM-EDS and FTIR. The effects of pH, initial mass concentration of tungsten, adsorption time and amount of adsorbent on the adsorption capacity were investigated. The adsorption mechanism was revealed by SEM-EDS and FTIR and XPS. The results showed that N-doped lignin was loose and porous and contained a large amount of phenolic hydroxyl, amine and quaternary ammonium functional groups. When the total concentration of tungsten was 0.005 mol/L and the value of pH was smaller than 4.7, the existing form of tungsten was H₂W₁₂O^6-₄₀. H₂W₁₂O^6-₄₀ was adsorbed by N-doped lignin through electrostatic attraction of hydrogen bond and coordination with amino and ion exchange with Cl^-. The saturated adsorption capacity of 1 g/L N-doped lignin for tungsten reached 421.68 mg/g at pH of 4.0, with initial tungsten mass concentration of 800 mg/L and adsorption time of 960 min. The adsorption followed Langmuir model and quasi-second-order kinetic model, indicating that the adsorption was monolayer homogeneous chemisorption.

Effect of ［BMIM］HSO₄ on physicochemical structure and thermal behavior of regenerated lignin

YANG Huamei, CHEN Ziqin, LUO Kai, WANG Beilei, YU Mengjun

2024, 44(3):  281-289.  DOI: 10.3969/j.issn.1000-1565.2024.03.007

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The effect of ionic liquid dissolution treatment on the thermal behavior of regenerated lignin was investigated. Taken regenerated lignin pretreated by 1-butyl-3-methylimidazole hydrogen sulfate(［BMIM］HSO₄)as the research object, the structure and composition of regenerated lignin were characterized by elemental analysis, XRD, FTIR, SEM. The thermal weight loss process of regenerated lignin was analyzed using a thermogravimetric analyzer and a distributed activation energy model. The effects of dissolution temperature and time on the structure and thermal weight loss process of ［BMIM］HSO₄ regenerated lignin were investigated. The results showed that the arrangement of lignin molecules is effectively adjusted during the lignin regeneration process, due to the occupying effect of ［BMIM］HSO₄. Compared with lignin raw materials, the thermal behavior of ［BMIM］HSO₄ regenerated lignin all shift towards the high-temperature range. Based on the calculation and analysis of the distributed activation energy model, the activation energy during the thermal weight loss process of regenerated lignin is higher than that of lignin raw materials. After the dissolution treatment of ［BMIM］HSO₄, the thermal stability of lignin is improved. The theoretical reference is provided for the development of lignin resource utilization technology.

Effect of INTS10 on cell cycle, apoptosis, growth and migration capacity of HCC

WANG Xueting, QI Xin, WEI Xiaojun, YANG Aiqing, ZHOU Gangqiao

2024, 44(3):  290-300.  DOI: 10.3969/j.issn.1000-1565.2024.03.008

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The effects of integrator complex subunits 10(INTS10)on cell cycle,apoptosis,growth and migration of hepatocellular carcinoma(HCC)cells and its potential molecular mechanism were explored. The HCC cell lines with stable overexpression or knockdown of INTS10 were obtained by lentivirus infection. The mRNA and protein expression levels of INTS10 were detected by qRT-PCR and Western blotting. The cell growth was detected by CCK-8, clonal formation and BrdU assay. The cell migration ability was detected by Transwell assay. Flow cytometry was used to detect the number of cell cycles and apoptosis. The results showed that overexpression of INTS10 could significantly inhibit the apoptosis, growth- DOI:10.3969/j.issn.1000-1565.2024.03.008INTS10对HCC细胞周期、凋亡、生长和迁移能力的影响王雪婷^1,2,齐欣³,魏小军⁴,杨爱清²,周钢桥^1,2,3(1.河北大学 化学与材料科学学院,河北 保定 071002;2.军事医学研究院 辐射医学研究所,北京 100850;3.贵州大学 医学院,贵州 贵阳 550025;4.航天中心医院 肝胆外科,北京 100049)摘 要:为了探究整合因子复合物亚基10(integrator complex subunits 10,INTS10 )对人肝细胞癌(hepatocellular carcinoma, HCC)细胞周期、凋亡、生长和迁移能力的影响及其潜在的分子作用机制,利用慢病毒感染法获得稳定过表达或敲低INTS10的HCC细胞系,采用qRT-PCR和Western blotting检测INTS10 mRNA和蛋白表达水平,接着采用 CCK-8法、克隆形成和BrdU实验检测细胞生长情况,采用 Transwell小室实验检测细胞迁移能力,采用流式分析术检测细胞的周期和凋亡.结果显示:过表达INTS10可显著抑制HCC细胞的凋亡、生长和迁移能力,促进G1期细胞数量的增加,而敲低INTS10则呈现相反的表型.通过通路富集分析发现,周期相关通路被显著富集,过表达INTS10后,CDC25A和CDK4的mRNA和蛋白质水平显著减少,而CDKN1A的水平显著增加,敲低INTS10则呈现相反趋势.综上,本研究初步揭示了INTS10在HCC细胞中可能通过影响G1/S期相关蛋白质的表达而发挥抑癌基因的功能,为下一步更为深入的功能和机制研究提供了基础.关键词:肝细胞癌(HCC);整合因子复合物亚基10(INTS10);CDC25A;CDKN1A;CDK4中图分类号:Q279 文献标志码:A 文章编号:1000-1565(2024)03-0290-11Effect of INTS10 on cell cycle, apoptosis, growth and migration capacity of HCC WANG Xueting^1,2, QI Xin³, WEI Xiaojun⁴, YANG Aiqing², ZHOU Gangqiao^1,2,3(1. College of Chemistry and Materials Science, Hebei University, Baoding 071002, China; 2. Institute of Radiation Medicine,Academy of Military Medical Sciences, Beijing 100850, China; 3. Medical College, Guizhou University, Guiyang 550025, China; 4. Department of Hepatobiliary Surgery,Space Center Hospital, Beijing 100049,China)Abstract: The effects of integrator complex subunits 10(INTS10)on cell cycle,apoptosis,growth and migration of hepatocellular carcinoma(HCC)cells and its potential molecular mechanism were explored. The HCC cell lines with stable overexpression or knockdown of INTS10 were obtained by lentivirus infection. The mRNA and protein expression levels of INTS10 were detected by qRT-PCR and Western blotting. The cell growth was detected by CCK-8, clonal formation and BrdU assay. The cell migration ability was detected by Transwell assay. Flow cytometry was used to detect the number of cell cycles and apoptosis. The results showed that overexpression of INTS10 could significantly inhibit the apoptosis, growth- 收稿日期:2023-05-11;修回日期:2023-06-15 基金项目:国家自然科学基金青年基金资助项目( 8190061543) 第一作者:王雪婷(1998—),女,河北大学硕士研究生,主要从事遗传学和整合组学研究.E-mail:1379402926@qq.com 通信作者:周钢桥(1972—),男,军事医学研究院研究员,主要从事遗传学和整合组学研究.E-mail:zhougq114@126.com第3期王雪婷等:INTS10对HCC细胞周期、凋亡、生长和迁移能力的影响and migration of HCC, and promote the increase of the number of G1 phase cells, while knockdown of INTS10 showed the opposite effects. Through pathway enrichment analysis, it was found that the cycle-dependent pathway was significantly enriched. Overexpression of INTS10 could significantly reduce the mRNA and protein levels of CDC25A and CDK4, while the level of CDKN1A was significantly increased, and INTS10 knockdown showed the opposite trend. In conclusion, it is preliminarily revealed that INTS10 may play a role as a tumor suppressor gene in HCC by affecting the expression of G1/S phase related proteins, which provides a foundation for further functional and mechanism studies.

Heshouwuyin mediates DNMT1 to regulate DNA methylation to promote rat Leydig cell function

SHI Bingye, LIU Xinru, WU Tian, YANG Yujiao, ZHEN Xiaolan, NIU Siyun

2024, 44(3):  301-311.  DOI: 10.3969/j.issn.1000-1565.2024.03.009

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To investigate the effect of Heshouwuyin-mediated gene epigenetic(DNA methylation)changes on testosterone secretion in rat Leydig cells, the testicular tissues of 12-month-old and 18-month-old Wistar male rats were selected. The genes AXL, Ttc29, wnt2b, MAPK10 and C-myb regulated by Heshouwuyin and closely related to spermatogenesis were screened by methylation chip. MSP and RT-qPCR were used to detect gene methylation level and mRNA expression level. BrdU fluorescence staining- DOI:10.3969/j.issn.1000-1565.2024.03.009何首乌饮介导DNMT1调控DNA甲基化促进大鼠Leydig细胞功能石炳烨^1,2,刘鑫如²,吴恬²,杨玉娇²,甄晓兰³,牛嗣云²(1.河北大学附属医院 超声诊断科,河北 保定 071000;2.河北大学 基础医学院, 河北 保定 071000;3.河北省药品医疗器械检验研究院 体外诊断试剂室,河北 石家庄 050000)摘 要:为探讨何首乌饮介导基因表观遗传(DNA甲基化)的改变对大鼠睾丸间质细胞(Leydig)分泌睾酮的影响,选用12、18月龄 Wistar雄性大鼠睾丸组织,甲基化芯片筛选出受何首乌饮调控并与精子发生功能密切的基因AXL、Ttc29、wnt2b、MAPK10、C-myb,甲基化特异性PCR(MSP)与实时荧光定量PCR(RT-qPCR)技术检测基因甲基化水平及mRNA表达水平;BrdU荧光染色观察细胞增殖情况;慢病毒转染敲低Leydig细胞DNMT1,分析wnt2b、C-myb甲基化程度和睾酮分泌水平是否受DNMT1影响.结果显示:何首乌饮可调控衰老大鼠睾丸组织中关键基因DNA甲基化和mRNA水平,促进细胞增殖和睾酮分泌;敲低Leydig细胞DNMT1后,细胞分泌睾酮水平降低,同时wnt2b和C-myb DNA甲基化水平也明显下降.这表明何首乌饮可能介导基因DNA甲基化程度促进大鼠Leydig细胞分泌睾酮,进而提高生精功能.关键词:何首乌饮;睾丸组织;DNA甲基化;睾酮中图分类号:Q291 文献标志码:A 文章编号:1000-1565(2024)03-0301-11Heshouwuyin mediates DNMT1 to regulate DNA methylation to promote rat Leydig cell functionSHI Bingye^1,2, LIU Xinru², WU Tian², YANG Yujiao², ZHEN Xiaolan³, NIU Siyun²(1. Department of Ultrasound Diagnosis, Afiliated Hospital of Hebei University,Baoding 071000, China;2. School of Basic Medical Sciences, Hebei University, Baoding 071000, China;3. In Vitro Diagnostic Reagent Room, Hebei Pharmaceutical and Medical Device Inspection and Research Institute, Shijiazhuang 050000, China)Abstract: To investigate the effect of Heshouwuyin-mediated gene epigenetic(DNA methylation)changes on testosterone secretion in rat Leydig cells, the testicular tissues of 12-month-old and 18-month-old Wistar male rats were selected. The genes AXL, Ttc29, wnt2b, MAPK10 and C-myb regulated by Heshouwuyin and closely related to spermatogenesis were screened by methylation chip. MSP and RT-qPCR were used to detect gene methylation level and mRNA expression level. BrdU fluorescence staining- 收稿日期:2023-08-11;修回日期:2023-11-10 基金项目:国家自然科学基金资助项目(81673714);河北省重点研发计划项目-中医药创新专项(223777134D);河北省高等学校科学技术研究项目(ZD2022060);河北省中医药管理局科研计划项目(2022115) 第一作者:石炳烨(1983—),男,河北大学附属医院主治医师,河北大学在读硕士研究生,主要从事男性生殖衰老研究.E-mail:2435231348@qq.com 通信作者:牛嗣云(1967—),女,满族,河北大学教授,博士,博士生导师,主要从事生殖衰老方向研究.E-mail:nsy1688@163.com甄晓兰(1976—),女,河北省药品医疗器械检验研究院副主任药师,主要从事生殖衰老方向研究.E-mail:zhenxlan2007@163.com第3期石炳烨等:何首乌饮介导DNMT1调控DNA甲基化促进大鼠Leydig细胞功能was used to observe cell proliferation. DNMT1 was knocked down by lentivirus transfection in Leydig cells, and the effects of DNMT1 on wnt2b and C-myb methylation and testosterone secretion were analyzed. The results showed that Heshouwuyin could regulate the DNA methylation and mRNA levels of key genes in testicular tissue of aging rats, and promote cell proliferation and testosterone secretion. After knocking down DNMT1 in Leydig cells, the level of testosterone secretion was decreased, and the levels of wnt2b and C-myb DNA methylation were also significantly decreased, indicating that Heshouwuyin may improve the ability of rat testicular cells to secrete testosterone by regulating the level of gene DNA methylation, thereby improving the spermatogenic function.

Community structure characteristics of macrozooplankton in the sea area near Dagu River Estuary of Jiaozhou Bay in summer and autumn

ZHANG Liang, ZHANG Shaoping, TAO Huihui, SU Kai, PU Sichao, JI Yinglu

2024, 44(3):  312-321.  DOI: 10.3969/j.issn.1000-1565.2024.03.010

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In order to know the ecological status of macrozooplankton in the sea area near Dagu River Estuary of Jiaozhou Bay, based on the investigation data in June(summer)of 2021 and September(autumn)of 2022, the community structure of macrozooplankton were analyzed. As result, a tolal of 20- DOI:10.3969/j.issn.1000-1565.2024.03.010胶州湾大沽河口邻近海域夏、秋季大型浮游动物群落结构特征张亮,张绍萍,陶卉卉,宿凯,蒲思潮,纪莹璐(自然资源部北海预报减灾中心 山东省海洋生态环境与防灾减灾重点实验室,山东 青岛 266061)摘 要:为了解胶州湾大沽河口附近海域的大型浮游动物生态状况,以2021年6月(夏季)和2022年9月(秋季)在胶州湾大沽河口邻近海域进行的大型浮游动物调查数据为基础,对该区域大型浮游动物群落结构进行分析,结果如下:该调查海域2021年夏季和2022年秋季总共发现大型浮游动物成体20种,浮游幼虫11类,其中,2021年夏季调查发现成体16种、浮游幼虫11类,2022年秋季发现成体10种、浮游幼虫5类;夏、秋两季调查大型浮游动物平均个体密度分别为184.6、221.3 ind/m³;平均生物量(湿重)为8 220.1、860.0 mg/m³;平均多样性指数为2.76、2.64;2021年夏季调查优势种为卡玛拉水母(Malagazzia carolinae)、黑球真唇水母(Eucheilota menoni)、日本真瘤水母(Proboscidactyla flavicirrata)、太平洋纺锤水蚤(Acartia pacifica)、长尾类幼虫(Macrura larva)、多毛类幼虫(Polychaeta larva)、磁蟹溞状幼虫(Porcellana Zoea larva)、短尾类溞状幼虫(Brachyura Zoea larva),2022年秋季调查优势种为卡玛拉水母(Malagazzia carolinae)、黑球真唇水母(Eucheilota menoni)、日本真瘤水母(Proboscidactyla flavicirrata)、球型侧腕水母(Pleurobrachia globosa )、薮枝螅水母(Obelia spp.)、强壮滨箭虫(Aidanosagitta crassa)、磁蟹溞状幼虫(Porcellana Zoea larva)、腹足类幼体(Gastropoda larva);聚类分析表明大沽河口大型浮游动物可划分为2个组.本研究可为大沽河口邻近海域大型浮游动物的演变等研究提供一定的参考.关键词:大沽河口;浮游动物;群落结构;优势种中图分类号:Q145 文献标志码:A 文章编号:1000-1565(2024)03-0312-10Community structure characteristics of macrozooplankton in the sea area near Dagu River Estuary of Jiaozhou Bay in summer and autumnZHANG Liang, ZHANG Shaoping, TAO Huihui, SU Kai, PU Sichao, JI Yinglu(Shandong Provincial Key Laboratory of Marine Ecology and Environment & Disaster Prevention and Mitigation, North China Sea Marine Forecasting and Hazard Mitigation Center of Ministry of Natural Resources, Qingdao 266061, China)Abstract: In order to know the ecological status of macrozooplankton in the sea area near Dagu River Estuary of Jiaozhou Bay, based on the investigation data in June(summer)of 2021 and September(autumn)of 2022, the community structure of macrozooplankton were analyzed. As result, a tolal of 20- 收稿日期:2023-05-15;修回日期:2023-06-20 基金项目:山东省海洋生态环境与防灾减灾重点实验室开放基金资助项目(202311);自然资源部北海局海洋科技项目(202302) 第一作者:张亮(1982—),男,自然资源部北海预报减灾中心高级工程师,主要从事海洋环境监测和评价研究. E-mail:36302162@qq.com 通信作者:纪莹璐(1990—),女,自然资源部北海预报减灾中心工程师,主要从事海洋环境监测和评价及底栖生态学研究. E-mail:jiyinglu@ncs.mnr.gov.cn第3期张亮等:胶州湾大沽河口邻近海域夏、秋季大型浮游动物群落结构特征species and 11 taxa of zooplankton larva, including 16 species and 11 taxa of zooplankton larva in summer and 10 species and 5 taxa of zooplankton larva in autumn, were recorded. The macrozooplankton average density was 184.6 ind/m³ in summer and 221.3 ind/m³ in autumn, the average biomass(wet weight)in summer was 8 220.1 mg/m³ and 860.0 mg/m³ in autumn, the average Shannon index(H')were 2.76 in summer and 2.64 in autumn, respectively. In summer, dominant species were Malagazzia carolinae, Eucheilota menoni, Proboscidactyla flavicirrata, Acartia pacifica, Macrura larva, Polychaeta larva, Porcellana Zoea larva, Brachyura Zoea larva, while in autumn they were Malagazzia carolinae, Eucheilota menoni, Proboscidactyla flavicirrata, Pleurobrachia globosa, Obelia spp., Aidanosagitta crassa, Porcellana Zoea larva, Gastropoda larva. Cluster analysis showed that the macrozooplankton in the investigation area can be divided into two groups. This article can provide important reference data for the evolution of macrozooplankton in the sea area near Dagu River Estuary.

A blockchain-based trusted data sharing scheme for electronic medical records

YANG Xiaohui, JIA Kai

2024, 44(3):  322-328.  DOI: 10.3969/j.issn.1000-1565.2024.03.011

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In order to address the current inadequacies of patient privacy and data security in medical blockchain, a blockchain-based trusted sharing scheme with user privacy as the center is proposed with the help of blockchain and key exchange technology. Combining key exchange technology and smart contracts for data key verification and trusted exchange, data security, trusted sharing and protection of rights and interests of both parties in the transaction are realized. Experiments and theories have proved that compared with existing schemes, this scheme has low consumption and high throughput, and has certain advantages and applicability.

Node importance identification in airline network based on multi-order neighborhood contribution degree

HU Gang, WANG Lemeng, LU Zhiyu, HU Junjie, KANG Kai

2024, 44(3):  329-336.  DOI: 10.3969/j.issn.1000-1565.2024.03.012

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In order to improve the robustness of airline network, the node importance identification of airline network is studied. A airline network topology model is constructed based on airline flight data, and multi-order heterogeneity information of the neighborhood is gathered by means of interaction order and average path difference between the nodes of the airline network. The circle structure of the neighborhood nodes of the airline network is used to characterize the closeness of the nodes in the network. A fusion model of multi-order neighborhood information and structural information of nodes is constructed based on airline network and a node centrality algorithm is proposed based on multi-order neighborhood contribution. The attack resources R=0.3 and R=0.5 are selected for the analysis, which maximally improve the attack utility value by 39.62% and 49.69%, respectively, indicating that the algorithm is accurate and effective in identifying the importance of nodes in the airline network, and it can provide theoretical references for the optimal design of the connectivity of the airline network.