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Schiff base reaction-induced fluorescence quenching of carbon dots for detection of formaldehyde
- CHANG Zhiwei, LIU Xiaotong, WANG Shoulin, YANG Yang, SHEN Shigang, DONG Jiangxue
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2025, 45(1):
50-59.
DOI: 10.3969/j.issn.1000-1565.2025.01.006
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In this work, the yellow fluorescent carbon dots(o-PD-CDs)was prepared by the microwave assisted method with o-phenylenediamine(o-PD)and N,N-dimethylformamide as raw materials, the preparation method is simple and rapid. Further study found that the yellow emission of o-PD-CDs would be quenched by formaldehyde(FA)in aqueous solution, and the quenching value of fluorescence intensity showed a good liner relationship with the FA concentration in the range from 0.01 to 0.80 mg/L, therefore, a fluorescent sensor was constructed for the quantitative detection of FA in aqueous solution and the limit of detection was 5.2 μg/L. Moreover, the FA sensing mechanism was explored, and the results showed that the o-PD-CDs possessed a lot of amino groups on the surface, a Schiff base reaction- DOI:10.3969/j.issn.1000-1565.2025.01.006席夫碱反应诱导的荧光碳点猝灭用于检测甲醛常志伟1,刘晓彤1,王守林1,杨阳1,申世刚1,2,董江雪1(1.河北大学 化学与材料科学学院,河北 保定 071002;2.京津冀(雄安新区)生态安全与生态保护教育部工程研究中心,河北 保定 071002)摘 要:以富含碳元素和氮元素的邻苯二胺和N,N-二甲基甲酰胺为原料,在微波辅助下通过一锅法成功制备了发射黄色荧光的碳点(o-PD-CDs),该制备方法简单快速.实验发现,所制备碳点的黄色荧光在水溶液中可被甲醛(FA)猝灭,并在FA质量浓度为0.01~0.80 mg/L时,荧光强度猝灭值与FA质量浓度呈现较好的线性关系.以o-PD-CDs为探针构建了水溶液中定量检测FA的荧光传感器,最低检出限为5.2 μg/L.此外,对检测FA的传感机制进行了研究,表明o-PD-CDs 含有丰富的氨基,可与FA发生席夫碱反应并生成相应的亚胺,从而导致 o-PD-CDs 的荧光发生猝灭.进一步实验发现,随着FA含量的增加,溶液中出现明显的席夫碱沉淀,据此可以实现质量浓度在0.5~150 mg/mL内FA的可视化半定量检测.所建立的传感器已成功应用于食品样品中甲醛的测定,回收率为98.10%~ 107.86%,相对标准偏差为0.86%~2.71%.关键词:碳点;荧光传感器;甲醛检测;席夫碱反应中图分类号:O657.1 文献标志码:A 文章编号:1000-1565(2025)01-0050-10Schiff base reaction-induced fluorescence quenching of carbon dots for detection of formaldehydeCHANG Zhiwei1, LIU Xiaotong1, WANG Shoulin1, YANG Yang1, SHEN Shigang1,2, DONG Jiangxue1(1. College of Chemisitry and Materials Science, Hebei University, Baoding 071002, China; 2. Engineering Research Center of Ecological Safety and Conservation in Beijing-Tianjin-Hebei(Xiongan New Area)of MOE, Baoding 071002, China)Abstract: In this work, the yellow fluorescent carbon dots(o-PD-CDs)was prepared by the microwave assisted method with o-phenylenediamine(o-PD)and N,N-dimethylformamide as raw materials, the preparation method is simple and rapid. Further study found that the yellow emission of o-PD-CDs would be quenched by formaldehyde(FA)in aqueous solution, and the quenching value of fluorescence intensity showed a good liner relationship with the FA concentration in the range from 0.01 to 0.80 mg/L, therefore, a fluorescent sensor was constructed for the quantitative detection of FA in aqueous solution and the limit of detection was 5.2 μg/L. Moreover, the FA sensing mechanism was explored, and the results showed that the o-PD-CDs possessed a lot of amino groups on the surface, a Schiff base reaction- 收稿日期:2024-08-10;修回日期:2024-09-30 基金项目:河北省自然科学基金资助项目(B2024201050);河北大学研究与创新团队项目(IT2023A03);河北大学实验室开放项目(sy202217) 第一作者:常志伟(1999-),男,河北大学在读硕士研究生,主要从事食品安全评估与检验.E-mail:18855216698@163.com 通信作者:董江雪(1987-),女,河北大学副教授,博士,主要从事纳米传感器研究.E-mail:dongjxue@163.com申世刚(1964-),男,河北大学教授,博士,主要从事食品与环境分析研究.E-mail:shensg@hbu.edu.cn 第1期常志伟等:席夫碱反应诱导的荧光碳点猝灭用于检测甲醛河北大学学报(自然科学版) 第45卷would occur between the amino groups and FA, which led to the fluorescence quenching of o-PD-CDs. In addition, with the increase of FA concentration, the Schiff base precipitated obviously in the solution, and the visual semi-quantitative detection of FA in the range of 0.5—150 mg/mL could be realized. The established sensor has been successfully applied to the determination of FA in food samples with the recovery rate of 98.1%—107.86% and the relative standard deviations of 0.86%—2.71%.
