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Vibration characteristics analysis of the inner race fault of axlebox bearing under wheel-rail excitation DENG Feiyue,WANG Hongli,GAO Ruiyang,LI Hao Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 561-570.   DOI: 10.3969/j.issn.1000-1565.2023.06.001 Abstract （339）      PDF（pc） （2669KB）（253）       Knowledge map    Save Wheel-rail complex excitation has a significant effect on the dynamic behavior of faulty axlebox bearing during the train operation. Firstly, this paper uses the Universal Mechanism(UM)software to establish a vehicle-track dynamic model, and obtains the vertical, longitudinal and lateral loads of axlebox bearing under wheel-rail complex excitation based on the verification of vehicle models for stability, smoothness and safety. Secondly, the dynamic model of axlebox bearing with inner race spalling is established via Solidworks and ADAMAS softwares, and the accuracy of the proposed model is verified by comparison with the axlebox bearing experiments on the roll-vibration test bench. The dynamic simulation analysis shows that the contact load of the roller and inner race of the fault side is greater than that of the non-faulty side and the normal bearing, the cage vibration of the fault side is greater than that of- DOI:10.3969/j.issn.1000-1565.2023.06.001轮轨激励条件下轴箱轴承内圈故障振动特性分析邓飞跃1,2,王红力1,高瑞洋1,李浩1(1.石家庄铁道大学 机械工程学院,河北 石家庄 050043;2.河北省工程机械动力与传动控制重点实验室,河北 石家庄 050043)摘 要:高速列车运行过程中,轮轨复杂激励会对轴箱轴承的动力学行为产生不可忽视的影响.首先利用UM(universal mechanism)软件建立车辆-轨道动力学模型,在对车辆模型进行稳定性、平稳性和安全性验证的基础上,获取了复杂激励下轴箱轴承所受的垂向、纵向和横向载荷;然后,通过Solidworks软件和ADAMS软件建立了轴箱轴承内圈剥离故障动力学模型,通过与滚振实验台轴箱轴承实验对比,验证了所提模型的准确性.通过动力学仿真分析可知,轴箱轴承故障侧的滚子与内圈接触载荷大于非故障侧与正常轴承,故障侧保持架的振动大于非故障侧与正常轴承,内圈故障冲击加剧了轴承保持架与外圈的质心波动.最后,进一步对比考虑轮轨激励下与定载荷下故障轴承仿真结果发现,受轮轨激励的影响轴承内部各个元件间的接触载荷显著增大,轴承保持架与外圈质心运动轨迹盒维数显著增大.研究成果对揭示实际工况下高速动车组轴箱轴承内部元件振动特性规律具有重要意义.关键词:轴箱轴承;轮轨激励;振动特性;内圈故障中图分类号:TH17 文献标志码:A 文章编号:1000-1565(2023)06-0561-10Vibration characteristics analysis of the inner race fault of axlebox bearing under wheel-rail excitationDENG Feiyue1,2,WANG Hongli1,GAO Ruiyang1,LI Hao1(1.School of Mechanical Engineering, Shijiazhuang Tiedao University, Shijiazhuang 050043,China;2.Hebei Key Laboratory of Mechanical Power and Transmission Control,Shijiazhuang 050043,China)Abstract: Wheel-rail complex excitation has a significant effect on the dynamic behavior of faulty axlebox bearing during the train operation. Firstly, this paper uses the Universal Mechanism(UM)software to establish a vehicle-track dynamic model, and obtains the vertical, longitudinal and lateral loads of axlebox bearing under wheel-rail complex excitation based on the verification of vehicle models for stability, smoothness and safety. Secondly, the dynamic model of axlebox bearing with inner race spalling is established via Solidworks and ADAMAS softwares, and the accuracy of the proposed model is verified by comparison with the axlebox bearing experiments on the roll-vibration test bench. The dynamic simulation analysis shows that the contact load of the roller and inner race of the fault side is greater than that of the non-faulty side and the normal bearing, the cage vibration of the fault side is greater than that of- 收稿日期:2023-06-13 基金项目:国家自然科学基金资助项目(12272243); 河北省研究生专业学位精品教学案例库项目(KCJPZ2023037) 第一作者:邓飞跃(1985—),男,河北石家庄人,石家庄铁道大学副教授,博士,博士生导师,主要从事机电系统故障诊断与状态监测研究.E-mail:dengfy@stdu.edu.cn第6期邓飞跃等:轮轨激励条件下轴箱轴承内圈故障振动特性分析the non-faulty side and the normal bearing, and the fault impact aggravates the trajectory of the mass center fluctuation of the bearing cage and outer ring. Finally, further comparison of the simulation results under the consideration of wheel-rail excitation and the constant load condition reveals that the contact load between the bearing components is significantly increased by the wheel-rail excitation,and the trajectories of the mass center for the bearing cage and outer ring fluctuate more drastically. The research results are of great significance in revealing the vibration characteristics law of the bearing internal components of faulty axlebox bearing under actual operating conditions. Reference | Related Articles | Metrics | Comments（0）

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Progress on extraction, isolation and antitumor activity of seaweed polypeptides SHEN Jinyang,ZHANG Xuan,YU Yuanyuan,ZHANG Shicheng,SHI Liuqing,DONG Zibo Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 379-386.   DOI: 10.3969/j.issn.1000-1565.2023.04.006 Abstract （230）      PDF（pc） （578KB）（226）       Knowledge map    Save Seaweed is an important marine life and an important source of food for people. Extensive previous studies have confirmed that seaweed contains a lot of protein. However, due to the low content of polypeptides, polypeptides are difficult to be extracted and purified by the traditional methods. At present, enzymatic hydrolysis and chemical extraction are more commonly used for extraction, and chromatography and membrane separation are used for purification. A higher purity polypeptides could be obtained from seaweed, and its structure was identified using MS-related technique. Seaweed polypeptides have a strong effect against tumor, and the mechanisms are manifested in many aspects. In this paper, the latest extraction, purification and identification methods of seaweed polypeptides, as well as the latest research progress of their anti-tumor activity were reviewed, so as to provide reference for their further development and utilization. Reference | Related Articles | Metrics | Comments（0）

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Classroom personnel detection based on object detection and time series migration CONG Shuai,YANG Lei,HUA Zhenghao,YANG Xiaohui Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 432-441.   DOI: 10.3969/j.issn.1000-1565.2023.04.012 Abstract （147）      PDF（pc） （1826KB）（151）       Knowledge map    Save In order to solve the problem of efficient supervision and management and data analysis of classroom persons in the intelligent reconstruction of education, this paper combines the good characteristics of single-stage object detection algorithm and the good special extraction ability of convolutional neural networks(CNN), and proposes an improved YOLO object detection algorithm based on attention network and time series migration. Firstly, this paper performs frame-by-frame extraction and non-distortion image deflation on the source video stream, and performs image super-resolution processing- DOI:10.3969/j.issn.1000-1565.2023.04.012基于目标检测和迁移时间序列的教室人员检测丛帅1,杨磊2,华征豪3,杨晓晖2(1.河北大学 工商学院,河北 保定 071000;2.河北大学 教务处,河北 保定 071002;3.河北大学 网络空间安全与计算机学院,河北 保定 071000)摘 要:为了解决教育智能化重构中对于教室人员的高效监督管理和数据分析,结合单阶段目标检测算法的优良特性和卷积神经网络(convolutional neural networks,CNN)良好的特征提取能力,提出了一种基于注意力机制网络和迁移时间序列改进YOLO目标检测算法的教室人员目标检测算法.首先,对源视频流进行逐帧抽取和非畸变的图像放缩,通过生成对抗网络(generative adversarial network,GAN)进行图像超分辨处理;其次,对每帧图像进行多尺度采样和初步目标检测;然后,根据不同尺度得到的候选结果进行非极大抑制(non maximum suppression,NMS)以去除置信度较低的个体;之后,对候选结果进行融合,再使用交并比(intersection over union,IoU)进行重叠度计算以更新数据、去除重合或过于紧密的定位位置,然后将当前帧的检测结果与先前时间区间中的检测结果作为时间序列进行统计学数据迁移融合(time series migration,TSM)获得最后的检测结果.实验结果表明,本文方法不仅有效地提升了教室人员目标检测的准确率,并且可以进行实时检测.关键词:目标检测;多尺度图像特征;超分辨率;注意力机制网络;迁移时间序列中图分类号:TP391.41 文献标志码:A 文章编号:1000-1565(2023)04-0432-10Classroom personnel detection based onobject detection and time series migrationCONG Shuai1,YANG Lei2,HUA Zhenghao3,YANG Xiaohui2(1. Industrial and Commercial College, Hebei University, Baoding 071000, China;2. Office of Educational Administration, Hebei University, Baoding 071002, China;3. School of Cyber Security and Computer, Hebei University, Baoding 071000, China )Abstract: In order to solve the problem of efficient supervision and management and data analysis of classroom persons in the intelligent reconstruction of education, this paper combines the good characteristics of single-stage object detection algorithm and the good special extraction ability of convolutional neural networks(CNN), and proposes an improved YOLO object detection algorithm based on attention network and time series migration. Firstly, this paper performs frame-by-frame extraction and non-distortion image deflation on the source video stream, and performs image super-resolution processing- 收稿日期:2022-09-13 基金项目:国家自然科学基金面上项目(62172139);中国高校产学研创新基金资助项目(2021BCF03002) 第一作者:丛帅(1980—),男,河北保定人,河北大学工商学院讲师,主要从事大数据技术、图像处理方面研究.E-mail:congshuai@hbu.cn 通信作者:杨磊(1986—),男,河北保定人,河北大学助理研究员,主要从事教育技术、智慧教学方面研究.E-mail:403064340@qq.com第4期丛帅等:基于目标检测和迁移时间序列的教室人员检测by Generative Adversarial Network(GAN). Secondly, multi-scale sampling and preliminary object detection are performed for each frame, and then Non Maximum Suppression(NMS)is performed to remove the individuals with low confidence based on the candidate results obtained at different scales. Then, the candidate results are fused, and the overlap is calculated using intersection over union(IoU)to update the data and remove overlapping or too close localization locations. Finally, the detection results of the current frame and those of the previous time interval are used as a time series for statistal data migration to obtain the final detection results. The experimental results show that this method not only effectively improves the accuracy of classroom personnel target detection, but also enables real-time detection. Reference | Related Articles | Metrics | Comments（0）

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Residents electric larceny detection based on Pearson correlation coefficient and SVM GUO Liang,GUO Zixue,JIA Hongtao,FAN Ruoyu Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 357-363.   DOI: 10.3969/j.issn.1000-1565.2023.04.003 Abstract （137）      PDF（pc） （914KB）（136）       Knowledge map    Save The exist of residents electric larceny not only damages the economic benefits of power supply enterprises, but also affects the security of electric grid. With the rapid development of China’s digital economy and the improvement of electric acquisition system, electric larceny detected methods which are based on big data are updating constantly. The paper puts forward a new method which combines Pearson correlation coefficient, SMOTE algorithm and SVM. Firstly, the paper uses Pearson correlation coefficient to collect abnormal users’ effective power stealing data which are recorded, then SMOTE algorithm is used to enrich effective power stealing database. At last the paper builds a detection mathematical model based on the data base by SVM. By comparing the results of detection mathematical model and the reality situation of users, the validity and feasibility of the method is proved. The new method not only gives a new thought for electricity anti-stealing in power enterprises, but also improves the efficient of working team. Reference | Related Articles | Metrics | Comments（0）

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Upper bounds on the double Roman domination number in graphs HAO Guoliang,ZHUANG Wei,XIE Zhihong,ZENG Shuting Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 449-452.   DOI: 10.3969/j.issn.1000-1565.2023.05.001 Abstract （180）      PDF（pc） （334KB）（134）       Knowledge map    Save By analyzing the structure of a graph and using the graph parameters, such as the number of vertices, diameter, minimum degree and packing number, some new upper bounds of the double Roman domination number of a connected graph are obtained. Reference | Related Articles | Metrics | Comments（0）

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Chaos and pattern transformation of a spatiotemporal discrete predator-prey system LEI Weiqian,GUO Fenglu,HUANG Tousheng Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 346-356.   DOI: 10.3969/j.issn.1000-1565.2023.04.002 Abstract （136）      PDF（pc） （917KB）（123）       Knowledge map    Save The complex spatiotemporal dynamics of a kind of spatiotemporal discrete Leslie-Gower type predator-prey system is discussed. The bifurcation theorem is used to analyze the conditions under which the system undergoes flip bifurcation, Neimark-Sacker bifurcation and Turing bifurcation near the immobility point. The bifurcation process of the system is demonstrated by the bifurcation diagram and the maximum Lyapunov exponent, the pattern transition on the route chaos triggered by the bifurcation is revealed by the changes of spatial amplitude and space-time development. The results show that the pattern indicate a similar period doubling cascade process on the route chaos caused by flip bifurcation, and the system goes through a change from frozen chaos to defect turbulence. The pattern is mainly shaped like annular and spiral waves on the route chaos caused by the Neimark-Sacker bifurcation, and the pattern formed by Turing instability on the periodic and quasi-periodic attractors displays the ordered spatial and temporal banded structure. The pattern formed on the chaotic attractor shows the spatial amplitude changes- DOI:10.3969/j.issn.1000-1565.2023.04.002一类时空离散捕食系统的混沌与斑图转变类维倩,郭丰路,黄头生(华北电力大学 工程生态学与非线性科学研究中心,北京 102206)摘 要:探讨了一类时空离散Leslie-Gower型捕食系统的复杂时空动力学行为.运用分岔定理分析了该系统在不动点附近发生倍周期分岔、Neimark-Sacker分岔和图灵分岔的条件.通过分岔图和最大李雅普诺夫指数展示了系统的分岔过程,借助空间振幅和时空发展的变化揭示了由分岔引发混沌路径上的斑图转变规律.结果表明:在倍周期分岔引发的混沌路径上,斑图呈现类似的周期加倍级联过程,系统经历了从冻结混沌到缺陷湍流的变化;在Neimark-Sacker分岔引发的混沌路径上,斑图以环状和螺旋波状为主,在周期和拟周期吸引子上经图灵失稳形成的斑图仍会呈现有序的时空带状结构,在混沌吸引子上形成的斑图虽然呈现无序的空间振幅变化,但在时空发展变化中会呈现某种整体有序局部混乱的湍流状态.关键词:时空离散;捕食系统;分岔;混沌路径;斑图自组织 中图分类号:O175.13 文献标志码:A 文章编号:1000-1565(2023)04-0346-11Chaos and pattern transformation of a spatiotemporal discrete predator-prey systemLEI Weiqian,GUO Fenglu,HUANG Tousheng(Research Center for Engineering Ecology and Nonlinear Science, North China Electric Power University, Beijing 102206, China)Abstract: The complex spatiotemporal dynamics of a kind of spatiotemporal discrete Leslie-Gower type predator-prey system is discussed. The bifurcation theorem is used to analyze the conditions under which the system undergoes flip bifurcation, Neimark-Sacker bifurcation and Turing bifurcation near the immobility point. The bifurcation process of the system is demonstrated by the bifurcation diagram and the maximum Lyapunov exponent, the pattern transition on the route chaos triggered by the bifurcation is revealed by the changes of spatial amplitude and space-time development. The results show that the pattern indicate a similar period doubling cascade process on the route chaos caused by flip bifurcation, and the system goes through a change from frozen chaos to defect turbulence. The pattern is mainly shaped like annular and spiral waves on the route chaos caused by the Neimark-Sacker bifurcation, and the pattern formed by Turing instability on the periodic and quasi-periodic attractors displays the ordered spatial and temporal banded structure. The pattern formed on the chaotic attractor shows the spatial amplitude changes- 收稿日期:2022-11-28 基金项目:国家自然科学基金青年基金资助项目(11802093);国家水体污染控制与治理科技重大专项(2017ZX07101002) 第一作者:类维倩(1997—),女,山东泰安人,华北电力大学在读硕士研究生,主要从事非线性动力学方向研究.E-mail: 120202209059@ncepu.edu.cn 通信作者:黄头生(1985—),男,江西吉水人,华北电力大学副教授,主要从事非线性动力学与斑图动力学方向研究.E-mail: tous_huang@ncepu.edu.cn第4期类维倩等:一类时空离散捕食系统的混沌与斑图转变of disorder, however, some turbulence state with overall order and local chaos appears in the changes of space-time development diagram. Reference | Related Articles | Metrics | Comments（0）

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Fault detection method of bearings based on HHO-CNN LIU Yuxin, WU Wenbo, ZHANG Xiong, WAN Shuting Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 571-583.   DOI: 10.3969/j.issn.1000-1565.2023.06.002 Abstract （143）      PDF（pc） （2602KB）（120）       Knowledge map    Save Bearing is an important supporting component of drive system, and it is also the weak link of the whole system. The powerful feature extraction and learning ability of convolutional neural network(CNN)provides the possibility for the pattern recognition of the idler bearing. Aiming at the problems of low accuracy and slow rate of convergence caused by the super parameter problem in using CNN to process multi classification pattern recognition, a CNN classification model optimized based on HHO algorithm was proposed. Firstly, the bearing fault dataset is partitioned with different fault types and degrees, and initializes the CNN model parameters; Then, the HHO algorithm is used to optimize the CNN model, calculate fitness values, and obtain the number of units and iteration times of the fully connected layer; Finally,- DOI:10.3969/j.issn.1000-1565.2023.06.002基于HHO-CNN的轴承故障诊断方法研究刘玉鑫1,武文博2,张雄2,万书亭2(1.国家电投内蒙古能源有限公司 通辽霍林河坑口发电有限责任公司,内蒙古 通辽 028000;2.华北电力大学 机械工程系,河北 保定 071003)摘 要:轴承是传动系统重要的支撑部件,也是整个系统的薄弱环节,卷积神经网络(convolutional neural network,CNN)强大的特征提取和学习能力为轴承运行状态模式识别提供了可能性.针对CNN处理多分类模式识别过程中,由超参数问题引起的准确率低、收敛速度慢等问题,提出了一种基于哈里斯鹰优化(Harris hawks optimization,HHO)算法优化的CNN分类模型.首先,对不同故障类型和故障程度轴承故障数据集进行划分,初始化CNN模型参数;然后,使用HHO算法对CNN模型的超参数空间进行优化,计算适应度值并获取全连接层的单元数量和迭代次数;最后,利用优化后的CNN模型对轴承数据集进行模式识别.通过不同故障类型和故障程度轴承实验数据验证,表明HHO-CNN模型可以使得全连接层的单元数量和迭代次数迅速收敛,及时准确调整CNN的网络参数,提升分类器的性能,提高了故障模式识别准确性,增强了模型的稳定性.关键词:HHO;CNN;轴承;故障诊断中图分类号:TH133.3;TP181 文献标志码:A 文章编号:1000-1565(2023)06-0571-13Fault detection method of bearings based on HHO-CNNLIU Yuxin1, WU Wenbo2, ZHANG Xiong2, WAN Shuting2(1.Tongliao Huolinhe Kengkou Power Generation Co. Ltd., State Power Investment Inner Mongolia Energy Co. Ltd.,Tongliao 028000, China;2.Department of Mechanical Engineering, North China Electric Power University, Baoding 071003, China)Abstract: Bearing is an important supporting component of drive system, and it is also the weak link of the whole system. The powerful feature extraction and learning ability of convolutional neural network(CNN)provides the possibility for the pattern recognition of the idler bearing. Aiming at the problems of low accuracy and slow rate of convergence caused by the super parameter problem in using CNN to process multi classification pattern recognition, a CNN classification model optimized based on HHO algorithm was proposed. Firstly, the bearing fault dataset is partitioned with different fault types and degrees, and initializes the CNN model parameters; Then, the HHO algorithm is used to optimize the CNN model, calculate fitness values, and obtain the number of units and iteration times of the fully connected layer; Finally,- 收稿日期:2023-06-13 基金项目:国家自然科学基金资助项目(52105098);河北省自然科学基金资助项目(E2021502038) 第一作者:刘玉鑫(1979—),男,内蒙古通辽人,国家电投内蒙古能源有限公司高级工程师,主要从事电力系统科技创新与管理研究.E-mail:zxlvbzy@163.com 通信作者:张雄(1990—),男,河北保定人,华北电力大学副教授,博士,主要从事旋转机械设备状态检测与故障诊断研究. E-mail:hdjxzx@ncepu.edu.cn第6期刘玉鑫等:基于HHO-CNN的轴承故障诊断方法研究the optimized CNN model is used for pattern recognition on the bearing dataset. Through experimental data of bearings, it is shown that the HHO-CNN model can quickly converge the number of units and iteration times in the fully connected layer, adjust the network parameters of the CNN in a timely and accurate manner, improve the accuracy of fault pattern recognition, and enhance the stability of the model. Reference | Related Articles | Metrics | Comments（0）

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M self-conjugate mixed structure solutions of the quaternion generalized Sylvester equation XU Yun,HUANG Jingpin Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 337-345.   DOI: 10.3969/j.issn.1000-1565.2023.04.001 Abstract （156）      PDF（pc） （428KB）（119）       Knowledge map    Save In this paper, using the specific structure M self-conjugate matrix in the form of quarto-block matrix, we discuss a class of M self-conjugate mixed structure solutions of generalized Sylvester equation AX-YB=C on the quaternion field, where X is a unitary similar block diagonal M self-conjugate matrix, Y is a self-conjugate matrix. According to the characteristics of the proposed structure matrix, the original structural equation is transformed into an equivalent system of unconstrained set of equations, and the necessary and sufficient conditions for the solution of the equations and the general solution expression are obtained by using the Moore-Penrose generalized inverse of the matrix. Thus, the M self-conjugate mixed structure solution of the original equation is obtained. In particular, the necessary and sufficient conditions for matrix equation AX=C to have unitary similar block diagonal M self-conjugate solutions and the general solution expression are derived. When M=0, the constrained mixed structure solution set of generalized Sylvester equation satisfying ‖Y~‖=min‖Y‖ is obtained by using the CCD-Q decomposition of quaternion matrix pairs. A numerical example is given to verify the correctness and feasibility of the results.- DOI:10.3969/j.issn.1000-1565.2023.04.001四元数广义Sylvester方程M自共轭混合结构解徐云,黄敬频(广西民族大学 数学与物理学院,广西 南宁 530006)摘 要:利用矩阵的四分块形式刻画了M自共轭矩阵的特征结构,并讨论了四元数广义Sylvester方程AX-YB=C的一类M自共轭混合结构解,其中X为酉相似块对角M自共轭矩阵,Y为自共轭矩阵.根据所提结构矩阵的特点,将原方程转化为等价的无约束方程组,再利用矩阵的Moore-Penrose广义逆,获得方程组可解的充分必要条件及其通解表达式,从而得到原方程的M自共轭混合结构解.特别地,导出矩阵方程AX=C具有酉相似块对角M自共轭解的充要条件及其通解表达式.当M=0时,利用四元数矩阵对的CCD-Q分解,获得广义Sylvester方程满足‖Y~‖=min‖Y‖的约束混合结构解集.数值算例检验了所得结果的正确及可行性.关键词:四元数;广义Sylvester方程;M自共轭;混合结构解中图分类号:O241.6 文献标志码:A 文章编号:1000-1565(2023)04-0337-09M self-conjugate mixed structure solutions of the quaternion generalized Sylvester equationXU Yun,HUANG Jingpin(College of Mathematics and Physics, Guangxi Minzu University, Nanning 530006, China)Abstract: In this paper, using the specific structure M self-conjugate matrix in the form of quarto-block matrix, we discuss a class of M self-conjugate mixed structure solutions of generalized Sylvester equation AX-YB=C on the quaternion field, where X is a unitary similar block diagonal M self-conjugate matrix, Y is a self-conjugate matrix. According to the characteristics of the proposed structure matrix, the original structural equation is transformed into an equivalent system of unconstrained set of equations, and the necessary and sufficient conditions for the solution of the equations and the general solution expression are obtained by using the Moore-Penrose generalized inverse of the matrix. Thus, the M self-conjugate mixed structure solution of the original equation is obtained. In particular, the necessary and sufficient conditions for matrix equation AX=C to have unitary similar block diagonal M self-conjugate solutions and the general solution expression are derived. When M=0, the constrained mixed structure solution set of generalized Sylvester equation satisfying ‖Y~‖=min‖Y‖ is obtained by using the CCD-Q decomposition of quaternion matrix pairs. A numerical example is given to verify the correctness and feasibility of the results.- 收稿日期:2022-10-07 基金项目:国家自然科学基金资助项目(11661011) 第一作者:徐云(1996—), 女, 贵州水城人, 广西民族大学在读硕士研究生, 主要从事数值代数方向研究.E-mail:960455313@qq.com 通信作者:黄敬频(1964—), 男, 广西陆川人, 广西民族大学教授, 主要从事数值代数方向研究. E-mail:hjp2990@126.com第4期徐云等:四元数广义Sylvester方程M自共轭混合结构解 Reference | Related Articles | Metrics | Comments（0）

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Application of novel ferroelectric thin films in MFIS devices WANG Shuyu,LU Chunguang, YUAN Qiuting,QIU Jiajun,FU Yueju,CAI Shuzhen,FU Guangsheng Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 364-368.   DOI: 10.3969/j.issn.1000-1565.2023.04.004 Abstract （108）      PDF（pc） （773KB）（111）       Knowledge map    Save Ferroelectric Na0.5Y0.5TiO3(NYTO)film is used as the insulating layer of MFIS(Metal-Ferroelectric-Insulate-Semiconductor)capacitors by its high dielectric properties. The Pt/ Pb(Zr0.2Ti0.8)O3/ NYTO/ Si structure capacitors were prepared, and their XRD, SEM, C-V, and I-V characterristics were tested and analyzed. The dependence of C-V storage window on application voltage and insulation layer thickness were studied separately. The results show that the device has a large memory window when the thickness of the insulation layer is 40 nm. The memory window can reach 13 V with 32 V applied voltage. The MFIS capacitor has low leakage current density of 1.08×10-6 A/cm2. The conduction mechanism of leakage current conforms to space charge limited current conduction mechanism. Reference | Related Articles | Metrics | Comments（0）

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Effect of Ga3+ on the luminescence property of Mg14Ge5O24:Cr near infrared material XU Yanmei,QIN Xiaodong,LIANG Shaolong,WANG Jiarui,WANG Xuejiao,WANG Zhijun Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 463-469.   DOI: 10.3969/j.issn.1000-1565.2023.05.003 Abstract （123）      PDF（pc） （1356KB）（89）       Knowledge map    Save Mg14Ge5O24:Cr near-infrared(NIR)phosphors were synthesized by the high-temperature solid-phase method to achieve the coexistence of Cr3+ and Cr4+ valence luminescence. The crystalline phases of the samples were analyzed using XRD and GASA refinement software. The material has two luminescence centers, one from Cr3+ occupying the octahedral Ge4+ position with an emission band in the NIR I region and a half-peak width up to 256 nm, and the other from Cr4+ occupying the tetrahedral Ge4+ position with an emission band in the NIR II region and a half-peak width up to 266 nm. The luminescence properties of the material were analyzed by low-temperature spectra and lifetime decay curves. The modulation of Cr3+ and Cr4+ luminescence can be achieved by using Ga3+ instead of Ge4+. The addition of large radius Ga3+ ions generates new lattice sites, which affects the overall crystal field intensity, and a phosphor with more intense luminescence in the NIR I region is obtained. Reference | Related Articles | Metrics | Comments（0）

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Effects of ALDH2 gene knockout on the development of male mice XU Wenxiu,LIU Qingling,LIU Xiaohan,KONG Lingying,SHI Hui,ZHAO Zhenjun Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 500-505.   DOI: 10.3969/j.issn.1000-1565.2023.05.008 Abstract （132）      PDF（pc） （1130KB）（77）       Knowledge map    Save In order to study the effects of ALDH2 gene knockout on the development of male mice, the effects of ALDH2 gene knockout on male mice were studied by analyzing the organ mass fraction of liver, kidney and testis, histomorphology, tissue antioxidant capacity and total litter size of mice at different weeks of age. The results showed that the cords of hepatocytes of Knockout type(KO)mice at 7 and 10 weeks of age were disordered. At 10 weeks of age, the number of multinucleated hepatocytes, and the black deposits in hepatocytes increased significantly. The kidneys of KO mice at 7 and 10 weeks of age showed obvious interstitial edema. In 7 and 10 weeks of aged mice, the sequence of late spermatogenic cells and spermatogenic cells in testicular seminiferous tubules were disordered. The total antioxidant capacity of liver and kidney in 10 weeks of age KO mice and the total antioxidant capacity of testis in 3 and 5 weeks of age KO mice were significantly lower than that of wild-type(WT)mice. The total number of pups in KO group was significantly less than that of WT mice. In conclusion, ALDH2 knockout can reduce the ability of anti-oxidative damage in liver, kidney and testis tissue of mice, result in cell damage, and affect the fertility of male mice in turn.The results showed that ALDH2 gene knockout would cause- DOI:10.3969/j.issn.1000-1565.2023.05.008乙醛脱氢酶ALDH2基因敲除对雄性小鼠发育的影响徐文秀,刘庆玲,刘笑含,孔令英,石慧,赵振军(烟台大学 生命科学学院,山东 烟台 264003)摘 要:为了研究乙醛脱氢酶ALDH2基因敲除对雄性小鼠发育的影响,将不同周龄ALDH2基因敲除的雄性小鼠,通过分析其肝、肾、睾丸脏器质量分数,组织细胞形态,组织抗氧化能力,总产仔数等指标,探讨ALDH2基因敲除对雄性小鼠的影响.结果表明:7、10周龄敲除型(KO)小鼠肝细胞索排列紊乱,10周龄时,多核肝细胞数量明显增多,且肝细胞中黑色沉积物增加;7、10周龄KO小鼠肾脏出现明显的间质水肿;7、10周龄KO小鼠睾丸生精管内晚期精细胞和精子排序紊乱,腔内有脱落的生精细胞,睾丸组织出现纤维化;10周龄KO小鼠肝脏、肾脏总抗氧化能力和3、5周龄KO小鼠睾丸组织总抗氧化能力显著低于野生型(WT)小鼠;KO组小鼠产仔总数显著少于WT小鼠.综上,ALDH2敲除导致小鼠肝脏、肾脏和睾丸组织抗氧化损伤的能力降低,造成其细胞氧化损伤,进而影响雄性小鼠的生育力.该研究结果说明ALDH2基因敲除会引发雄性小鼠组织器官发育异常,进而影响小鼠的个体发育.关键词:乙醛脱氢酶2;肝脏;肾脏;睾丸;组织抗氧化;生育力中图分类号:Q954.6 文献标志码:A 文章编号:1000-1565(2023)05-0500-06Effects of ALDH2 gene knockout on the development of male miceXU Wenxiu,LIU Qingling,LIU Xiaohan,KONG Lingying,SHI Hui,ZHAO Zhenjun(School of Life Sciences,Yantai University,Yantai 264003,China)Abstract: In order to study the effects of ALDH2 gene knockout on the development of male mice, the effects of ALDH2 gene knockout on male mice were studied by analyzing the organ mass fraction of liver, kidney and testis, histomorphology, tissue antioxidant capacity and total litter size of mice at different weeks of age. The results showed that the cords of hepatocytes of Knockout type(KO)mice at 7 and 10 weeks of age were disordered. At 10 weeks of age, the number of multinucleated hepatocytes, and the black deposits in hepatocytes increased significantly. The kidneys of KO mice at 7 and 10 weeks of age showed obvious interstitial edema. In 7 and 10 weeks of aged mice, the sequence of late spermatogenic cells and spermatogenic cells in testicular seminiferous tubules were disordered. The total antioxidant capacity of liver and kidney in 10 weeks of age KO mice and the total antioxidant capacity of testis in 3 and 5 weeks of age KO mice were significantly lower than that of wild-type(WT)mice. The total number of pups in KO group was significantly less than that of WT mice. In conclusion, ALDH2 knockout can reduce the ability of anti-oxidative damage in liver, kidney and testis tissue of mice, result in cell damage, and affect the fertility of male mice in turn.The results showed that ALDH2 gene knockout would cause- 收稿日期:2022-10-20 基金项目:国家重点研发计划(2018YFC1003600) 第一作者:徐文秀(1996—),女,山东临沂人,烟台大学在读硕士研究生,主要从事雄性小鼠生殖发育研究.E-mail:xwx5935@163.com 通信作者:赵振军(1976—),男,山东烟台人,烟台大学副教授,博士,主要从事小鼠生殖发育研究. E-mail:zhaozhenjun@ytu.edu.cn第5期徐文秀等:乙醛脱氢酶ALDH2基因敲除对雄性小鼠发育的影响abnormal development of tissues and organs in male mice, and then affect the individual development of mice. Reference | Related Articles | Metrics | Comments（0）

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Charge of dielectric particle deep into the plasma sheath in DC discharge CAI Zhimin,ZHANG Ning,ZHANG Shunxin,WANG Shuo,LIU Fucheng,HE Yafeng Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 470-475.   DOI: 10.3969/j.issn.1000-1565.2023.05.004 Abstract （111）      PDF（pc） （905KB）（76）       Knowledge map    Save Aiming to understand the complex motion process of large dielectric particle in the DC discharge dusty plasma experiments, the electrical properties and charge of dielectric particle deep into the plasma sheath are studied by the theoretical analysis and numerical simulation. Results show that the electricity and the charge of large particle deep into the plasma sheath are completely different from that of the small particle suspended near the sheath boundary which were widely studied previously. When the distance between the particle and the electrode is less than 8.2 mm, the particle is positively charged. By modifying the calculation of ion charging current, the charge of particle with the radius of 250 μm is calculated to be about 5.2×106 e under the applied voltage of -280 V. This verifies previous experimental measurement results. Reference | Related Articles | Metrics | Comments（0）

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A robust suppressed fuzzy C-means image segmenting algorithm based on image patch ZHENG Yibo,LI Zhongcan,CHENG Yangxin,DONG Yuhe,ZHU Zhanlong Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 442-448.   DOI: 10.3969/j.issn.1000-1565.2023.04.013 Abstract （91）      PDF（pc） （1277KB）（76）       Knowledge map    Save Considering that the non-destructive testing(NDT)image is easy to be disturbed by noise and the target occupies a small area of the image, a robust and adaptive suppressed fuzzy C-means algorithm based on the image patch(SFCMP)is proposed to segment NDT image. Firstly, the weight of the pixels in the image patch is adaptively determined, which is affected by the spatial distance and gray value of each pixel in the image patch. Then the fuzzy uncertainty model of image patch is constructed, and it is introduced into the new objective function with image patch as the basic unit, and the implementation process of the SFCMP is given. Finally, experiments are carried out on the NDT images, and the results show that the SFCMP has good robustness and effectiveness. Reference | Related Articles | Metrics | Comments（0）

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Discharge characteristics of a linear-field brush-shaped plasma plume and surface modification of polymer HAN Guoxin,WU Jiacun,JIA Hanxiao,WANG Xuefang,JIA Pengying Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 369-378.   DOI: 10.3969/j.issn.1000-1565.2023.04.005 Abstract （125）      PDF（pc） （1471KB）（74）       Knowledge map    Save Atmospheric pressure plasma jets have the ability to generate a plasma plume with a large number of active species in open space, and have been extensively used for material growth, surface modification, biomedicine and et al. However, the small cross section size of plasma plume have limited the efficiency for plasma jet application. Therefore, it is needed to develop a plasma plume with large-scale. At present, the large-scale plasma plumes reported are mostly cross-field plasma jets, which are less chemically active than the linear-field plasma jets. Excited by an alternate-current voltage, an argon linear-field jet with two electrodes has been developed to generate a large-scale brush-shaped plasma plume.- DOI:10.3969/j.issn.1000-1565.2023.04.005平行场刷形等离子体羽的放电特性及其聚合物表面改性韩国新1,武珈存1,贾焓潇1,王雪芳2,贾鹏英1(1.河北大学 物理科学与技术学院,河北 保定 071002;2.石家庄铁道大学 经济管理学院,河北 石家庄 050043)摘 要:大气压等离子体射流可以在开放空间产生富含活性粒子的等离子体羽,在材料生长、表面改性、生物医疗等领域具有广泛的应用前景. 由于通常等离子体羽的截面尺度很小,造成等离子体射流应用时效率较低,因此,急需产生大尺度的等离子体羽. 目前报道的大尺度的等离子体羽多是交叉场等离子体羽,相比于平行场等离子体羽而言其化学活性较低. 针对于此,本文利用双电极结构的平行场氩气射流,通过交流激励产生了大尺度的刷形等离子体羽. 利用电学、光学和光谱学手段对刷形等离子体羽的放电特性进行了表征. 在此基础上,利用该平行场刷形等离子体羽对聚合物(聚对苯二甲酸乙二醇酯(PET))进行了改性研究. 通过研究PET表面的水接触角(WCA)、拉曼光谱、X线光电子能谱(XPS)和原子力显微镜(AFM)照片,发现刷形等离子体羽能实现PET表面的大尺度均匀改性. 改性后的PET表面含氧极性官能团数量显著增加,并且PET表面的粗糙度增加,因此亲水性得到改善, 此种亲水性改良对不同的聚合物具有普适性. 这些研究结果对于大尺度等离子体射流的深入研究及等离子体表面改性的应用均具有一定参考价值. 关键词:大气压刷形等离子体羽;平行场射流;聚合物表面改性中图分类号:O461.2 文献标志码:A 文章编号:1000-1565(2023)04-0369-10Discharge characteristics of a linear-field brush-shaped plasma plume and surface modification of polymerHAN Guoxin1,WU Jiacun1,JIA Hanxiao1,WANG Xuefang2,JIA Pengying1(1. College of Physics Science and Technology, Hebei University, Baoding 071002, China; 2. School of Economics and Management, Shijiazhuang Tiedao University, Shijiazhuang 050043, China)Abstract: Atmospheric pressure plasma jets have the ability to generate a plasma plume with a large number of active species in open space, and have been extensively used for material growth, surface modification, biomedicine and et al. However, the small cross section size of plasma plume have limited the efficiency for plasma jet application. Therefore, it is needed to develop a plasma plume with large-scale. At present, the large-scale plasma plumes reported are mostly cross-field plasma jets, which are less chemically active than the linear-field plasma jets. Excited by an alternate-current voltage, an argon linear-field jet with two electrodes has been developed to generate a large-scale brush-shaped plasma plume.- 收稿日期:2022-09-06 基金项目:河北省自然科学基金资助项目(HB17JY070);河北大学研究生创新资助项目(HBU2022bs004) 第一作者:韩国新(1997—),男,河北张家口人,河北大学在读硕士研究生,主要从事大气压低温等离子体射流研究.E-mail:hanguoxin2020@163.com 通信作者:贾鹏英(1976—),女,河北保定人,河北大学副教授,主要从事大气压等离子体源、电除尘器和气体放电实验方向研究.E-mail:plasmalab@126.com第4期韩国新等:平行场刷形等离子体羽的放电特性及其聚合物表面改性The discharge characteristics of brush-shaped plasma plume are characterized by electrical, optical and spectral methods. Besides, the linear-field brush-shaped plasma plume is used to modify surface of a polymer(polyethylene terephthalate(PET)). By studying the water contact angle(WCA), Raman spectrum, X-ray photoelectron spectrum(XPS)and atomic force microscopy(AFM)image of PET surface, it is found that the brush-shaped plasma plume can achieve large-scale uniform modification. After plasma modification, the amount of oxygen-containing groups on the surface of PET significantly increases, and the roughness of PET surface also increases. Hence, the hydrophilicity of plasma treated PET surface has been improved. This hydrophilic modification is universal to different polymers. These results are of great significance for the development of large-scale plasma jet and the application of plasma modification. Reference | Related Articles | Metrics | Comments（0）

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Screening, characterization and property evaluation of dipyridamole eutectic drug system LI Yuanchun,DONG Xueqing,LI Congwei,AN Qingqing,YANG Caiqin Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 387-394.   DOI: 10.3969/j.issn.1000-1565.2023.04.007 Abstract （94）      PDF（pc） （1381KB）（72）       Knowledge map    Save Two component binary phase diagram analysis method was used to screen the eutectic system of the insoluble drug dipyridamole, and DSC, PXRD and FTIR techniques were used to characterize it. Subsequently, the solubility and dissolution of dipyridamole in the eutectic system were studied, and the solubilization mechanism of the eutectic system was studied by fluorescence quenching and phase solubility methods. The results showed that dipyridamole and nicotinamide formed a “V” type binary phase diagram with a single melting point, which proved that they formed a eutectic system. The solubility and dissolution rate of dipyridamole were improved by this system. Fluorescence spectra showed that there was a complexation effect between dipyridamole and nicotinamide. The phase solubility results further showed that a soluble complex was formed between the two, and the molar ratio of 1∶1. The complexation constants at 25 ℃ and 37 ℃ were 150.22 L/mol and 85.14 L/mol, respectively. The complexation reaction was endothermic, and the temperature rise was not conducive to the complexation of dipyridamole and nicotinamide. Reference | Related Articles | Metrics | Comments（0）

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Effects of long-term continuous 9.375 GHz microwave radiation on Caenorhabditis elegans ZHANG Wenli, LI Zhihui, DONG Guofu, WEI Liya, WANG Changzhen Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 624-630.   DOI: 10.3969/j.issn.1000-1565.2023.06.0089.375 Abstract （70）      PDF（pc） （1028KB）（70）       Knowledge map    Save The Caenorhabditis elegans was used to explore the effects of 9.375 GHz microwave radiation on the motor ability, reproductive ability and lifespan of Caenorhabditis elegans. The wild-type strair(N2)and sod-3 mutant(gk235)strain of C. elegans were cultured to adulthood and synchronized. The worms were randomly divided into control and radiation groups((1 000±100)worms per group)when- DOI:10.3969/j.issn.1000-1565.2023.06.0089.375 GHz微波长期辐射对秀丽线虫的影响张文丽1,2,李志慧2,董国福2,魏丽亚1,王长振1,2(1.河北大学 生命科学学院,河北 保定 071002;2.军事医学研究院 辐射医学研究所,北京 100850)摘 要:利用模式生物秀丽线虫(Caenorhabditis elegans)探究9.375 GHz微波辐射对其运动能力、生殖能力和寿命的影响.将野生型株系(N2)和sod-3基因突变株系(gk235)秀丽线虫培养至成年期进行同步化处理,同步化后秀丽线虫长至L1期时,随机分为对照组和辐射组,每组(1 000±100)只,对辐射组进行连续不间断辐射(频率9.375 GHz,比吸收率(specific absorption ratio,SAR)4 W/kg,辐射时间24 h/d),直到秀丽线虫寿命结束为止.当辐射到L4期时,定期统计产卵量;辐射第7天和第10天,分别检测各组秀丽线虫的运动能力(头部摆动、身体弯曲和咽部震动次数)和存活时间.结果表明:辐射第7天,与对照组相比,N2株系秀丽线虫的头部摆动、身体弯曲和咽部震动次数均呈下降趋势,但无统计学差异;辐射第10天,与对照组相比,可显著降低N2株系秀丽线虫的头部摆动次数(P<0.05),而身体弯曲次数和咽部震动次数呈下降趋势,但无统计学差异.与对照组相比,长期辐射可显著降低sod-3基因突变株系秀丽线虫的头部摆动次数和身体弯曲次数(P<0.05),而咽部震动次数无统计学差异.与对照组相比,长期辐射对N2 和sod-3基因突变株系的产卵量和寿命均无明显影响.综上,在频率9.375 GHz、SAR 4 W/kg的条件下,长期持续辐射可降低秀丽线虫的运动能力,但对秀丽线虫的生殖和寿命无明显影响.本实验为后续深入研究X波段辐射损伤机体的作用机制提供参考.关键词:9.375 GHz微波;秀丽线虫;运动能力;生殖;寿命中图分类号:Q955;O552.424 文献标志码:A 文章编号:1000-1565(2023)06-0624-07Effects of long-term continuous 9.375 GHz microwave radiation on Caenorhabditis elegansZHANG Wenli1,2, LI Zhihui2, DONG Guofu2, WEI Liya1, WANG Changzhen1,2(1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China)Abstract: The Caenorhabditis elegans was used to explore the effects of 9.375 GHz microwave radiation on the motor ability, reproductive ability and lifespan of Caenorhabditis elegans. The wild-type strair(N2)and sod-3 mutant(gk235)strain of C. elegans were cultured to adulthood and synchronized. The worms were randomly divided into control and radiation groups((1 000±100)worms per group)when- 收稿日期:2022-10-21 基金项目:国家自然科学基金资助项目(31800053) 第一作者:张文丽(1997—),女,湖北枣阳人,河北大学在读硕士研究生,主要从事电磁辐射生物效应研究.E-mail:17343355261@163.com 通信作者:魏丽亚(1983—),女,河北石家庄人,河北大学副教授,主要从事生态基因组方向研究.E-mail: weiliya@hbu.edu.cn王长振(1976—),男,山东济宁人,军事医学研究院副研究员,主要从事电磁辐射生物效应方向研究.E-mail: wangcz2002@aliyun.com第6期张文丽等:9.375 GHz微波长期辐射对秀丽线虫的影响they hatched to the L1 phase and started continuous uninterrupted radiation(radio frequency is 9.375 GHz, specific absorption rate(SAR)is 4 W/kg, radiation time is 24 h/d)until the end of worms life. When C. elegans was radiated to the L4 stage, the number of eggs was counted regularly every day. At the time points on the 7th and 10th day of radiation, the motor ability(the number of head swings, body bends, pharyngeal pump vibrations)and survival time of C. elegans in each group were measured, respectively. The results showed that after the 7th day of radiation, the number of head swings, body bends and pharyngeal pump vibrations of the N2 strain showed a decreasing trend compared to the control group, but there was no statistical difference. After the 10th day of radiation, the number of head swings of the N2 strain was significantly reduced compared to the control(P<0.05), while the number of body bends and pharyngeal vibrations tended to decrease, but they were not statistically different. Long-term radiation significantly reduced the number of head swings and body bends in the sod-3 mutant strain compared to the control group(P<0.05), while the number of pharyngeal vibrations was not statistically different. No significant effect on egg production and lifespan of the N2 and sod-3 mutant strains compared to the control group after radiation was observed. In conclusion, under the condition 9.375 GHz and radiation dose of 4 W/kg, long-term continuous radiation can significantly reduce the motor ability of C. elegans, but it has no significant effect on the reproduction and lifespan of C. elegans. This experiment provides a reference for further study of the mechanism of the X-band radiation damage to the organism. Reference | Related Articles | Metrics | Comments（0）

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Analysis of the characteristics of decomposition release of aquatic plants in Baiyangdian Lake TONG Jikun,ZHAO Jianguo,WANG Junxia,LI Hongbo,CHEN Haiying,WANG Hongyun Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 424-431.   DOI: 10.3969/j.issn.1000-1565.2023.04.011 Abstract （115）      PDF（pc） （1126KB）（69）       Knowledge map    Save To study the decomposition release characteristics of Baiyangdian Lake aquatic plants during decay, a multidimensional survey of Baiyangdian Lake aquatic plants was conducted using a combination of remote sensing interpretation and field surveys to estimate the decomposition release of total nitrogen, total phosphorus and organic matter from typical dominant aquatic plant Potamogeton crispus, - DOI:10.3969/j.issn.1000-1565.2023.04.011白洋淀水生植物腐解释放特征分析佟霁坤1,赵建国2,3,王军霞4,李洪波2,3,陈海婴1,王红云5(1.白洋淀流域生态环境监测中心,河北 保定 071051;2.河北省生态环境科学研究院 绿色发展研究所,河北 石家庄 050000;3.河北省水环境科学实验室,河北 石家庄 050000;4.河北大学 生命科学学院, 河北 保定 071002;5.保定市生态环境监控中心,河北 保定 071002)摘 要:为研究白洋淀水生植物衰败期腐解释放特征,采用遥感解译和现场调查相结合的方法,对白洋淀水生植物进行了多维度调查,估算了典型优势水生植物菹草、篦齿眼子菜和莲的全氮、全磷、有机质腐解释放量,提出了白洋淀水生植物管理对策.结果表明:菹草、篦齿眼子菜和莲的腐解释放对白洋淀水质影响较大;易腐解组分在总生物量中的质量分数菹草为72%,篦齿眼子菜为40%,莲为12.14%;难腐解组分在总生物量中的质量分数菹草为28%,篦齿眼子菜为60%,莲为87.86%.优势水生植物全氮、全磷、有机质的释放效率不同,但所有植物腐解前期磷释放速率最快,菹草有机质的释放量最大.水生植物腐解释放具有明显的时间特征,与水生植物生长周期密切相关,集中在优势种的腐解时期.为控制白洋淀水生植物腐解释放污染,应根据水生植物的生长周期及腐解释放规律,在5月中旬对菹草进行收割,9月中下旬对莲和篦齿眼子菜进行收割,并拓展水生植物的资源化利用途径.关键词:白洋淀;水生植物;腐解释放;生长周期中图分类号:Q89 文献标志码:A 文章编号:1000-1565(2023)04-0424-08Analysis of the characteristics of decomposition release of aquatic plants in Baiyangdian LakeTONG Jikun1,ZHAO Jianguo2,3,WANG Junxia4,LI Hongbo2,3,CHEN Haiying1,WANG Hongyun5(1. Baiyangdian Basin Ecological Environment Monitoring Center,Baoding 071051, China;2. Institute of Green Development,Hebei Institute of Ecological and Environmental Sciences,Shijiazhuang 050000, China;3. Hebei Water Environmental Science Laboratory, Shijiazhuang 050000, China;4. College of Life Sciences,Hebei University, Baoding 071002,China;5. Baoding Ecological Environmental Monitoring Center,Baoding 071002, China)Abstract: To study the decomposition release characteristics of Baiyangdian Lake aquatic plants during decay, a multidimensional survey of Baiyangdian Lake aquatic plants was conducted using a combination of remote sensing interpretation and field surveys to estimate the decomposition release of total nitrogen, total phosphorus and organic matter from typical dominant aquatic plant Potamogeton crispus, - 收稿日期:2022-09-14 基金项目:河北省重点研发计划项目资源与环境创新专项(3110701);河北省湿地生态与保护重点实验室开放基金资助项目(hklk202003);保定市科技计划项目(2141ZF003) 第一作者:佟霁坤(1981—),男,河北怀来人,白洋淀流域生态环境监测中心高级工程师,主要从事水生态监测研究.E-mail:601766712@qq.com 通信作者:赵建国(1991—),男,河北石家庄人,河北省生态环境科学研究院工程师,主要从事水生态环境修复方向研究.E-mail:807078293@qq.com王军霞(1974—),女,河北新乐人,河北大学副教授,主要从事水域生态修复方向研究. E-mail: 303370613@qq.com第4期佟霁坤等:白洋淀水生植物腐解释放特征分析P.pectinatus and Nelumbo nucifera,countermeasures for the management of Baiyangdian Lake aquatic plants were proposed.The results showed that the decomposing release of P. crispus, P. pectinatus and N. nucifera had a greater impact on Baiyangdian Lake water quality. The mass fraction of easily decomposed fractions in the total biomass was 72% for P. crispus, 40% for P. pectinatus cress and 12.14% for N. nucifera; the mass fraction of difficult to decompose fractions in the total biomass was 28% for P. crispus, 60% for P. pectinatus and 87.86% for N. nucifera.The release efficiency of total nitrogen, total phosphorus, and organic matter from dominant aquatic plants varied, but the release rate of phosphorus was fastest in all plants during the pre-decay period, and the release of organic matter was greatest in P. crispus.The decay release of aquatic plants has obvious temporal characteristics, closely related to the growth cycle of aquatic plants, concentrated in the decay period of the dominant species. In order to control the decay and release of aquatic plants in Baiyangdian Lake, we should harvest P. crispus in mid-May and P. pectinatus and N. nucifera in mid-late September according to the growth cycle and decay and release rules of aquatic plants, and expand the resource utilization pathway of aquatic plants. Reference | Related Articles | Metrics | Comments（0）

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Periodic changes for adsorption of methyl oranges on natural hydrotalcite by online method YE Jianhua,YU Tao,YU Haoqi,LIU Huiyu,YUAN Xueyan Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 395-407.   DOI: 10.3969/j.issn.1000-1565.2023.04.008 Abstract （120）      PDF（pc） （2140KB）（68）       Knowledge map    Save In order to study the periodic change law of adsorbance behavior of pollutants in water on adsorbent surface, explore the dynamic change mechanism of adsorption and desorption, and improve the adsorption efficiency, the online adsorption experiment was carried out by using the home-made online spectrophotometer, combined with the online measurement results of pH and electrical conductivity of adsorption system. The kinetic and thermodynamic properties of the adsorption of methyl orange on natural hydrotalcite were explained. Based on theoretical calculations, the equation of the periodic variation of adsorption was proposed and deduced. The 721 spectrophotometer was modified for the online determination of the real-time changes of adsorbent concentration in the adsorption system; the optimal online adsorption condition was determined by the experiment condition at pH 3.5 and solid-liquid ratio of 0.002 g/L, and the adsorption rate under this condition reached 83.6%. The online adsorption experiments- DOI:10.3969/j.issn.1000-1565.2023.04.008甲基橙在天然水滑石上的在线吸附周期性规律研究叶剑华1,于涛1,余豪奇1,刘会誉1,原雪燕2(1.东华理工大学 核科学与工程学院,江西 南昌 330013;2.东华理工大学 化学生物与材料科学学院,江西 南昌 330013)摘 要:为研究水体中的污染物在吸附剂表面吸附性行为的周期性变化规律,探讨吸附-解吸的动态变化机理,提高吸附效率,采用改造的分光光度计进行在线吸附实验,结合吸附体系pH、电导率的在线测定结果,解释了甲基橙在天然水滑石上的吸附动力学、热力学特性.根据理论计算,提出并推导出吸附周期性变化规律公式.通过改造的721分光光度计,在线测定吸附体系中吸附质浓度的实时变化;通过条件实验确定最佳在线吸附条件为pH 3.5,吸附剂质量浓度0.002 g/L,在此条件下的吸附率达83.6%.在线吸附实验显示,天然水滑石对甲基橙的在线吸附过程中,前10 min吸附率持续增长,10~15 min吸附率增长趋于平稳,15~20 min吸附率变化呈指数衰减,符合指数衰减规律.此后吸附量随接触时间的变化呈现出潮汐波动现象.此潮汐波动呈周期性变化,且变化周期受温度和浓度影响较小,每个周期的变化符合指数增长规律.关键词:甲基橙;天然水滑石;吸附;周期性变化;动力学中图分类号:X52 文献标志码:A 文章编号:1000-1565(2023)04-0395-13Periodic changes for adsorption of methyl oranges on natural hydrotalcite by online methodYE Jianhua1,YU Tao1,YU Haoqi1,LIU Huiyu1,YUAN Xueyan2(1.School of Nuclear Science and Engineering, East China University of Technology, Nanchang 330013, China; 2. School of Chemical Biology and Materials Science, East China University of Technology, Nanchang 330013, China)Abstract: In order to study the periodic change law of adsorbance behavior of pollutants in water on adsorbent surface, explore the dynamic change mechanism of adsorption and desorption, and improve the adsorption efficiency, the online adsorption experiment was carried out by using the home-made online spectrophotometer, combined with the online measurement results of pH and electrical conductivity of adsorption system. The kinetic and thermodynamic properties of the adsorption of methyl orange on natural hydrotalcite were explained. Based on theoretical calculations, the equation of the periodic variation of adsorption was proposed and deduced. The 721 spectrophotometer was modified for the online determination of the real-time changes of adsorbent concentration in the adsorption system; the optimal online adsorption condition was determined by the experiment condition at pH 3.5 and solid-liquid ratio of 0.002 g/L, and the adsorption rate under this condition reached 83.6%. The online adsorption experiments- 收稿日期:2022-07-12 基金项目:国家自然科学基金资助项目(21561001);江西省自然科学基金资助项目(20202BABL203004) 第一作者:叶剑华(1998—),男,云南曲靖人,东华理工大学在读硕士研究生,主要从事环境放射化学方向研究. E-mail:2513172134@qq.com 通信作者:于涛(1979—),男,吉林白城人,东华理工大学副教授,博士,主要从事环境放射化学方向研究.E-mail:xiaoshan770@163.com第4期叶剑华等:甲基橙在天然水滑石上的在线吸附周期性规律研究showed that the adsorption rate of natural hydrotalcite on methyl orange continued to increase in the first 10 min, and then stabilized from 10 to 15 min and declined exponentially from 15 to 20 min, which was in accordance with the exponential decay law. After that, the adsorption amount showed a tidal fluctuation with the contact time. The tidal fluctuation was cyclical, and the cycle of change was less affected by temperature and concentration, and the change of each cycle was in accordance with the exponential growth law. Reference | Related Articles | Metrics | Comments（0）

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Influence of temperature on the production of monascin and ankaflavin by Monascus ruber M7 during liquid state fermentation XU Yong,QIU Ziya,CHEN Suo,ZHANG Jialan,GAO Mengxiang,LI Li Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 408-419.   DOI: 10.3969/j.issn.1000-1565.2023.04.009 Abstract （116）      PDF（pc） （4011KB）（65）       Knowledge map    Save To improve the production of monascin(MS)and ankaflavin(AF)by Monascus ruber M7, the changes in composition and yield of alcohol-soluble pigments produced at different fermentation temperatures were investigated using liquid state fermentation. It was found that pigments produced at lower temperatures(25、30 ℃)were orange in hue, with a maximum absorption around 470 nm, and- DOI:10.3969/j.issn.1000-1565.2023.04.009温度对红色红曲菌M7液态发酵产monascin和ankaflavin的影响徐勇1,邱子娅1,陈莎1,2,张佳兰3,高梦祥1,2,李利1,2(1.长江大学 生命科学学院,湖北 荆州 434025;2.长江大学 食品研究院,湖北 荆州 434025;3.长江大学 动物科学学院,湖北 荆州 434025)摘 要:为了提高红色红曲菌(Monascus ruber)M7发酵生产红曲素(monascin, MS)和红曲黄素(ankaflavin, AF)的产量,采用液态发酵法,探究不同发酵温度下其产生的醇溶性色素在组成和产量方面的变化规律.结果发现,当温度较低时(25、30 ℃),所产生的色素在470 nm有最大吸收,色调偏橙,红斑素(rubropunctatin, RP)和红曲红素(monascorubrin, MR)为优势组分;当温度较高时(35、40 ℃),所产生的色素在400 nm 附近有最大吸收,色调偏黄,MS和AF成为优势组分.随着温度的升高,所有色素组分的产量均先升高后降低,其中,25、40 ℃不利于色素的产生,产量显著低于30、35 ℃;30 ℃时,RP和MR的产量达到最大值,分别为107、124 mg/L,而MS和AF的产量较低,分别为48、26 mg/L;35 ℃时,MS和AF的产量达到最大值,分别为100、47 mg/L,而RP和MR的产量相对30 ℃时显著降低,分别为57、50 mg/L.qPCR分析发现,温度对MS和AF液态发酵的影响与其对色素合成关键酶基因的转录调控密切相关,其中,对色素合成分支节点关键酶基因MrPigF和MrPigH的差异调节,可能对35 ℃时MS和AF产量的显著提高起到了较关键的作用.该结果可为提高MS和AF液态发酵产量提供参考.关键词:红色红曲菌;红曲素;红曲黄素;液态发酵;温度中图分类号:TS201.3 文献标志码:A 文章编号:1000-1565(2023)04-0408-12Influence of temperature on the production of monascin and ankaflavin by Monascus ruber M7 during liquid state fermentationXU Yong1,QIU Ziya1,CHEN Suo1,2,ZHANG Jialan3,GAO Mengxiang1,2,LI Li1,2(1. College of Life Science, Yangtze University, Jingzhou 434025, China; 2. Institute of Food Science and Technology, Yangtze University, Jingzhou 434025, China; 3. College of Animal Science, Yangtze University, Jingzhou 434025, China)Abstract: To improve the production of monascin(MS)and ankaflavin(AF)by Monascus ruber M7, the changes in composition and yield of alcohol-soluble pigments produced at different fermentation temperatures were investigated using liquid state fermentation. It was found that pigments produced at lower temperatures(25、30 ℃)were orange in hue, with a maximum absorption around 470 nm, and- 收稿日期:2022-06-07 基金项目:国家自然科学基金资助项目(31730068) 第一作者:徐勇(1997—),男,四川达州人,长江大学在读硕士研究生,主要从事红曲色素的发酵及其在食品中的应用研究. E-mail:1903437308@qq.com 通信作者:李利(1983—),女,湖北浠水人,长江大学副教授,博士,主要从事真菌次生代谢产物及其在食品中的应用研究. E-mail:lily2012@yangtzeu.edu.cn第4期徐勇等:温度对红色红曲菌M7液态发酵产monascin和ankaflavin的影响rubropunctatin(RP)and monascorubrin(MR)were the dominant components; while pigments produced at higher temperatures(35、40 ℃)were yellow in hue, with a maximum absorption around 400 nm, and MS and AF became the dominant ones. As the temperature increased, the yields of each pigment compounds first increased and then decreased. The yields of all pigment compounds were significantly lower at 25、40 ℃ than at 30、35 ℃, indicating that these temperatures were not conducive to the production of pigments. At 30 ℃, RP and MR had a maximum yield of 107、124 mg/L, respectively, while MS and AF had a lower yield, being 48、26 mg/L, respectively. At 35 ℃, MS and AF reached a maximum yield of 100、47 mg/L, while RP and MR decreased significantly when compared to 30 ℃, being 57、50 mg/L, respectively. qPCR analysis revealed that the influence of temperature on the liquid state fermentation of MS and AF was closely related to its transcriptional regulation on key genes responding to pigments biosynthesis. The differential regulation on MrPigF and MrPigH, the key enzyme genes of the pigments biosynthesis branch node, might playe a critical role in improving the yields of MS and AF at 35 ℃. These results would be helpful for the promotion of MS and AF during liquid state fermentation. Reference | Related Articles | Metrics | Comments（0）

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Application of object detection algorithm in pathology diagnosis of lung cancer WU Jianguo,YANG Xiaoru,WANG Pan,WU Junfang,LI Ruikai Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 546-552.   DOI: 10.3969/j.issn.1000-1565.2023.05.014 Abstract （80）      PDF（pc） （1596KB）（62）       Knowledge map    Save Object detection based on deep learning has been widely studied in many fields such as transportation and military, and has achieved remarkable results. In order to further study its usability in medical image diagnosis, two classical models of object detection algorithms, SSD and Faster RCNN, are proposed to appliy to the lesion detection of lung cancer pathological images. The experimental comparison shows that the average processing time of Faster RCNN is about 4.6 times longer than that of SSD, but the recognition accuracy and precision are higher than that of SSD, and the model performance of Faster RCNN is much better. The research results show that the object detection algorithm can realize the intelligent diagnosis of lung cancer pathological images and improve the diagnosis rate of lung cancer. Reference | Related Articles | Metrics | Comments（0）

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Isolation and identification of secondary metabolites of Fusarium equiseti PAN Huayang, LIU Huaguang, QI Zexin, ZHAO Xiaolong, LUO Duqiang Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 512-516.   DOI: 10.3969/j.issn.1000-1565.2023.05.010 Abstract （78）      PDF（pc） （625KB）（61）       Knowledge map    Save In order to find compounds with new structures and significant pharmacological effects, three compounds 1, 2 and 3 were isolated and identified from the secondary metabolites of the endophytic fungus Fusarium equiseti from Harmonia axyridis by modern isolation methods. Among them, compounds 1(equisetin)and 2(epi-equisetin)are a pair of epiisomers, and 3(6-methoxy-droperylene)belongs to steroids. In vitro protein tyrosine phosphatase 1B(PTP1B)inhibitory activity test showed that compounds 1 and 2 had certain inhibitory activity on PTP1B, and IC50 values were(8.73 ± 0.03),(12.88 ± 0.16)μg/mL. It provides potential drug lead compounds for the development of new PTP1B inhibitors. Reference | Related Articles | Metrics | Comments（0）

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Effect of nitrite on start-up of anammox EGSB reactor SHI Yonghui, ZHANG Yihao, QIN Zhe, SHI Tianxi, WU Xinying, ZHAO Chunxia, LU Da Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 595-602.   DOI: 10.3969/j.issn.1000-1565.2023.06.005 Abstract （79）      PDF（pc） （1098KB）（60）       Knowledge map    Save Long-term stored anaerobic ammonia oxidation sludge at 4 ℃ was inoculated to investigate the effect of influent nitrite on Anammox expanded granular sludge bed(EGSB)reactor start-up at 50.0 mg/L(R1)and 70.0 mg/L(R2). It was found that the retardation time of R1 was 18 days shorter than that of R2, and higher concentrations of nitrite(70.0 mg/L)inhibited the start-up of the Anammox EGSB reactor. R1 and R2 were successfully started at 53 d and 56 d, with total nitrogen(TN)removal rates of 67.9% and 68.8%, respectively. After increasing the nitrogen load, the ammonia removal rates of R1 and R2 increased from 93.1% and 88.0%, respectively, to 99.6% and 99.0%. The hydraulic shock experiment discovered that external circulation could enhance the substrate mixing effect in the reactor and improve the effluent water quality, and R1 and R2 recovered on the 5th and 6th days following destabilization, respectively. High-throughput sequencing revealed that the dominant genus in both reactors was Candidatus_Jettenia with a relative abundance of 54.1% and 45.3%, respectively. Long-term stored Anammox sludge was initiated with a nitrite concentration recommended below 50.0 mg/L. Reference | Related Articles | Metrics | Comments（0）

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Design of cooling system of air warm plasma jet generator LIU Zhenhua, HUANG Baowang, LI Zhanxian Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 584-588.   DOI: 10.3969/j.issn.1000-1565.2023.06.003 Abstract （83）      PDF（pc） （717KB）（60）       Knowledge map    Save A gas loop warm plasma jet generator with air as the working gas as well as cooling media was designed by optimizing the generator electrode and cooling structure in order to better meet the requirements of portable, simple operation, and diverse application backgrounds of plasma jet generator. The impacts of gas flow rate, discharge frequency, and discharge voltage on plasma jet temperature were studied. It was found that the introduction of cooling gas obviously reduces the jet temperature. Under the conditions of 5.5 kV voltage, 22 kHz frequency and 7 L/min flow rate, the introduction of cooling gas reduced the jet temperature by 302 ℃. The jet temperature decreased with the increase of gas flow rate and discharge frequency, but increased with the increase of discharge voltage. The decrease of jet temperature was more dependent on gas flow rate than discharge frequency or voltage. Reference | Related Articles | Metrics | Comments（0）

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Fault diagnosis of rolling bearing based on improved Autogram LIU Shangkun, ZHANG Wei, SUN Yuhao, KONG Degang, ZHAO Xiaoshun Journal of Hebei University(Natural Science Edition)    2024, 44 (1): 1-8.   DOI: 10.3969/j.issn.1000-1565.2024.01.001 Abstract （77）      PDF（pc） （1340KB）（60）       Knowledge map    Save To address the problem of inaccurate selection of demodulation frequency band and difficulty in fault diagnosis that may arise when using the unbiased autocorrelation kurtosis index, the square envelope negentropy is introduced into Autogram, and an improved Autogram method using square envelope negentropy index is proposed to select the optimal demodulation frequency band. The square envelope negentropy is less affected by noise and can measure the impact of period signal. Firstly, the bearing vibration signal is decomposed into different frequency bands by maximum overlapping discrete wavelet packet transform. Then the square envelope negentropy of each frequency band is calculated, and the optimal frequency band which has the maximum square envelope negentropy value is selected. Finally, the bearing fault type is diagnosed by analyzing the square envelope spectrum of the optimal demodulation frequency band. The analysis results of fault bearing experimental signals by improved Autogram, Autogram, and fast kurtogram method showed that the improved Autogram method is more reasonable in the selecting demodulation frequency band, and more obvious in extracting fault frequency, and more accurately in diagnosing bearing faults.- DOI:10.3969/j.issn.1000-1565.2024.01.001基于改进Autogram的滚动轴承故障诊断刘尚坤,张伟,孙宇浩,孔德刚,赵晓顺(河北农业大学 机电工程学院,河北 保定 071001)摘 要:针对Autogram以无偏自相关峭度指标选取解调频带时易出现不准确、故障诊断困难的问题,将平方包络负熵引入Autogram中,结合平方包络负熵受噪声影响小且能衡量周期冲击成分的优点,提出一种采用平方包络负熵指标选取最优解调频带的改进Autogram方法.首先采用最大重叠离散小波包变换(MODWPT)对轴承振动信号进行频带分解,再计算每个频带信号的平方包络负熵,将平方包络负熵最大的频带作为最优解调频带,最后通过分析最优解调频带平方包络谱的频率成分来诊断轴承故障类型.将轴承故障实验信号分析结果与Autogram、快速谱峭度方法对比,结果表明,改进Autogram方法在解调频带的选取上更为合理,所提取的故障特征频率更明显,轴承故障诊断更准确.关键词:平方包络负熵;改进Autogram;最优解调频带;故障诊断中图分类号:TH133.33 文献标志码:A 文章编号:1000-1565(2024)01-0001-08Fault diagnosis of rolling bearing based on improved AutogramLIU Shangkun, ZHANG Wei, SUN Yuhao, KONG Degang, ZHAO Xiaoshun(School of Mechanical and Electrical Engineering, Hebei Agricultural University, Baoding 071001, China)Abstract: To address the problem of inaccurate selection of demodulation frequency band and difficulty in fault diagnosis that may arise when using the unbiased autocorrelation kurtosis index, the square envelope negentropy is introduced into Autogram, and an improved Autogram method using square envelope negentropy index is proposed to select the optimal demodulation frequency band. The square envelope negentropy is less affected by noise and can measure the impact of period signal. Firstly, the bearing vibration signal is decomposed into different frequency bands by maximum overlapping discrete wavelet packet transform. Then the square envelope negentropy of each frequency band is calculated, and the optimal frequency band which has the maximum square envelope negentropy value is selected. Finally, the bearing fault type is diagnosed by analyzing the square envelope spectrum of the optimal demodulation frequency band. The analysis results of fault bearing experimental signals by improved Autogram, Autogram, and fast kurtogram method showed that the improved Autogram method is more reasonable in the selecting demodulation frequency band, and more obvious in extracting fault frequency, and more accurately in diagnosing bearing faults.- 收稿日期:2023-05-06;修回日期:2023-09-20 基金项目:河北省重点研发计划项目(22327206D);保定市科技计划项目(2172P003);河北省省属高校基本科研业务费资助项目(KY202014);河北农业大学引进人才科研专项(YJ201814) 第一作者:刘尚坤(1979—),男,河北农业大学副教授,博士,主要从事机械设备状态监测与故障诊断方向研究.E-mail:lsk1213@163.com 通信作者:孔德刚(1986—),男,河北农业大学讲师,主要从事机械设计制造及故障诊断方向研究.E-mail:870714576@qq.com第1期刘尚坤等:基于改进Autogram的滚动轴承故障诊断 Reference | Related Articles | Metrics | Comments（0）

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Establishment and application of polysaccharide characteristic degradation profile for Yupingfeng oral liquid MA Mengnan,ZHANG Yu,LI Xinke,LIU Jinbao,LIU Dan,GUO Huaizhong Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 616-623.   DOI: 10.3969/j.issn.1000-1565.2023.06.007 Abstract （79）      PDF（pc） （1091KB）（58）       Knowledge map    Save The characteristic degradation profile of Yupingfeng oral liquid polysaccharide(YPF-P)was established in this paper, and the quality control of this preparation was improved based on the profile. YPF-P was obtained by the precipitation of 70% ethanol solution, and the excipient sucrose in the oral liquid was removed in the meantime. The optimized partial degradation conditions of YPF-P were as follows: 0.5 mol/L hydrochloric acid solution, temperature 80 ℃, time 4 h. The degradation products derived by 1-phenyl-3-methyl-5-pyrazolinone(PMP)were separated by capillary zone electrophoresis(CZE)under the following conditions: uncoated fused silica capillary column(50 cm×50 μm i.d., effective length 41 cm)was used, and the running buffer was 40 mmol/L borax buffer(pH 10.1), detection wavelength was set at 245 nm with operating voltage 10 kV, gravity injection 15 cm×20 s was employed, and the column temperature was room temperature. According to the L9(34)orthogonal design,- DOI:10.3969/j.issn.1000-1565.2023.06.007玉屏风口服液多糖特征降解谱的建立和应用马梦楠1,张钰1,李欣珂1,刘进宝1,刘丹1,郭怀忠1,2(1.河北大学 药学院,河北 保定 071002;2.河北省药物质量分析控制重点实验室,河北 保定 071002)摘 要:建立了玉屏风口服液多糖(YPF-P)的特征降解谱,并基于此完善该制剂的质量控制,通过体积分数70%乙醇溶液沉淀得到YPF-P,同时去除口服液中的辅料蔗糖.优化的YPF-P部分降解条件为0.5 mol/L盐酸溶液,温度80 ℃,时间4 h,随后采用毛细管区带电泳法(CZE)对经过1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生后的YPF-P部分降解产物进行测定.CZE条件为未涂层熔融石英毛细管柱(50 cm × 50 μm i.d.,有效长度41 cm),运行缓冲液40 mmol/L硼砂缓冲液(pH 10.1),检测波长245 nm,运行电压10 kV,重力进样15 cm × 20 s,柱温为室温.根据L9(34)正交设计组合不同来源的玉屏风处方药材,制备9份玉屏风模拟样品,采用中位数法综合各特征降解谱得到YPF-P特征降解谱对照谱图,并对不同批次样品YPF-P特征降解谱进行聚类分析.结果表明:不同来源和组合的YPF-P特征降解谱差异显著,证明多糖特征降解谱可用于YPF-P成分的分析,也可为其他中药复方制剂多糖成分质量控制的深入研究提供参考.关键词:玉屏风口服液;多糖;特征降解谱;毛细管区带电泳法;中药复方制剂中图分类号:R917 文献标志码:A 文章编号:1000-1565(2023)06-0616-08Establishment and application of polysaccharide characteristic degradation profile for Yupingfeng oral liquidMA Mengnan1,ZHANG Yu1,LI Xinke1,LIU Jinbao1,LIU Dan1,GUO Huaizhong1,2(1. College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; 2. Key Lab of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China)Abstract: The characteristic degradation profile of Yupingfeng oral liquid polysaccharide(YPF-P)was established in this paper, and the quality control of this preparation was improved based on the profile. YPF-P was obtained by the precipitation of 70% ethanol solution, and the excipient sucrose in the oral liquid was removed in the meantime. The optimized partial degradation conditions of YPF-P were as follows: 0.5 mol/L hydrochloric acid solution, temperature 80 ℃, time 4 h. The degradation products derived by 1-phenyl-3-methyl-5-pyrazolinone(PMP)were separated by capillary zone electrophoresis(CZE)under the following conditions: uncoated fused silica capillary column(50 cm×50 μm i.d., effective length 41 cm)was used, and the running buffer was 40 mmol/L borax buffer(pH 10.1), detection wavelength was set at 245 nm with operating voltage 10 kV, gravity injection 15 cm×20 s was employed, and the column temperature was room temperature. According to the L9(34)orthogonal design,- 收稿日期:2023-01-17 基金项目:河北省自然科学基金资助项目(H2021201018;H2016201221);河北省创新能力计划项目(20567605H) 第一作者:马梦楠(1996—),女,河北石家庄人,河北大学在读硕士研究生.E-mail:mmn_0721@163.com 通信作者:郭怀忠(1968—),男,内蒙古商都人,河北大学教授,博士,主要从事中药质量控制及其药效学方向研究.E-mail: ghuaizh@aliyun.com第6期马梦楠等:玉屏风口服液多糖特征降解谱的建立和应用nine Yupingfeng simulated samples were prepared by combining the prescription medicinal materials with different sources of Yupingfeng. The reference YPF-P characteristic degradation profile was obtained by synthesizing the above nine profiles using median method, and the cluster analysis of the characteristic degradation profiles of YPF-P from different batches was carried on. The results indicated that the characteristic degradation profiles of YPF-P from different batches and simulated samples were significantly different, which proved that the characteristic degradation profiles of polysaccharide could be used for the analysis of YPF-P, and could also provide reference for the in-depth study of the quality control of polysaccharide in other Chinese herbal medicine compound preparations. Reference | Related Articles | Metrics | Comments（0）

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Effect of NiO photocathode film thickness on the performance of quantum dot sensitized solar cells WANG Senyang,ZHANG Zhaoyou,SUN Jie,LIANG Chengyang,CAO Ying,GENG Shitong,LI Ling Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 492-499.   DOI: 10.3969/j.issn.1000-1565.2023.05.007 Abstract （86）      PDF（pc） （1207KB）（58）       Knowledge map    Save This work is to explore the best preparation conditions of photoanode film materials in quantum dot sensitized solar cells(QDSSCs). P-type NiO nanoparticles are synthetized by one-step high temperature hydrothermal method, which is then screen printed on conductive glass to form NiO films. NiO films can be used as photocathode for quantum dot sensitized solar cells. Combine with Cu2S counter electrode, solar cells are assembled. NiO photocathode films with different film thicknesses were studied and tested repeatedly. The results show that the highest photoelectric conversion efficiency is achieved when the film thickness of NiO film material is 21 μm. Reference | Related Articles | Metrics | Comments（0）

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Preparation and characterization of glutamate iron chelate LIU Xinshuo,WANG Zhen,LI Qing,YANG Wenzhi,LI Haiying Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 476-483.   DOI: 10.3969/j.issn.1000-1565.2023.05.005 Abstract （123）      PDF（pc） （1302KB）（56）       Knowledge map    Save Glutamic acid(Glu)was reacted with trivalent iron ions(Fe3+)to prepare glutamate iron chelate ［Glu-Fe(Ⅲ)］. Glu-Fe(Ⅲ)was characterized by FT-IR, DSC, and TGA analysis. The Fe3+ content in Glu-Fe(Ⅲ)was determined by an indirect iodometric method and identified as an evaluation index for optimizing the preparation condition of Glu-Fe(Ⅲ). The effects of molar ratio of glutamic acid to iron, pH, time and temperature on the iron content of Glu-Fe(Ⅲ)were investigated by single factor method and Box-Benhnken design(BBD). The optimum preparation condition of Glu-Fe(Ⅲ)was Glu/Fe3+ molar ratio of 3∶5, pH 4.8, 50 ℃ and 3 h, and the iron content of Glu-Fe(Ⅲ)prepared under the optimized conditions was 19.91%. Glu-Fe(Ⅲ)could be a new iron and glutamic acid nutrient supplement. Reference | Related Articles | Metrics | Comments（0）

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Evaluation of uncertainty of measurement in determination of aflatoxin B1 content in polygala tenuifolia willd by HPLC MA Lihui,CUI Yan,LI Xun,ZHAO Zhilei Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 484-491.   DOI: 10.3969/j.issn.1000-1565.2023.05.006 Abstract （116）      PDF（pc） （500KB）（56）       Knowledge map    Save In order to evaluate the uncertainty of measurement in the determination of aflatoxin B1 in polygala tenuifolia willd by high performance liquid chromatography(HPLC),according to the national metrology technical specifications described in JJF1059.1—2012 “Evaluation and Expression of Uncertainty in Measurement”, six samples were randomly selected, and the mathematical model was established to analyze uncertainty sources,quantify the uncertainty components, calculate synthetic uncertainty and extend uncertainty in terms of the dilution of standard solution, the weighing of the samples, the final volume of the samples and the repeatability of instrumental measurement. The experimental results showed that the combined uncertainty of aflatoxin B1 determination in polygala7 tenuifolia- DOI:10.3969/j.issn.1000-1565.2023.05.006HPLC法测定远志中黄曲霉毒素B1含量的不确定度评定马力辉1,2,3,崔炎1,2,3,李迅1,2,3,赵志磊1,2,3,4(1.河北大学 质量技术监督学院,河北 保定 071002;2.计量仪器与系统国家地方联合工程研究中心,河北 保定 071002;3.河北省能源计量与安全检测技术重点实验室,河北 保定 071002;4.河北大学 地理标志研究院,河北 保定 071002 )摘 要:为评定高效液相色谱法测定远志中黄曲霉毒素B1含量的不确定度,依据国家计量技术规范 JJF 1059.1—2012《测量不确定度评定与表示》,对随机选取的6 个远志样品,建立数学模型,从标准溶液稀释、样品称量、样品最终定容体积和仪器测定重复性等方面分析不确定度来源,量化不确定度分量,计算合成不确定度和扩展不确定度.实验结果表明远志中黄曲霉毒素B1测定结果合成标准不确定度为0.016 39 μg/kg,扩展不确定度0.032 78 μg/kg.不确定度评定结果表明:HPLC法对远志中黄曲霉毒素B1含量的测定数据可靠,远志中黄曲霉毒素B1含量的测量不确定度主要来源于重复性实验、标准曲线的拟合、液相色谱仪的使用.关键词:不确定度;黄曲霉毒素B1;HPLC;远志 中图分类号:O657.72 文献标志码:A 文章编号:1000-1565(2023)05-0484-08Evaluation of uncertainty of measurement in determination of aflatoxin B1 content in polygala tenuifolia willd by HPLCMA Lihui1,2,3,CUI Yan1,2,3,LI Xun1,2,3,ZHAO Zhilei1,2,3,4(1.College of Quality and Technical Supervision, Hebei University, Baoding 071002, China;2. National&Local Joint Engineering Research Center of Metrology Instrument and System, Baoding 071002, China;3. Hebei Key Laboratory of Energy Metering and Safety Testing Technology, Baoding 071002, China;4.Institute of Geographical Indications,Hebei University, Baoding 071002, China)Abstract: In order to evaluate the uncertainty of measurement in the determination of aflatoxin B1 in polygala tenuifolia willd by high performance liquid chromatography(HPLC),according to the national metrology technical specifications described in JJF1059.1—2012 “Evaluation and Expression of Uncertainty in Measurement”, six samples were randomly selected, and the mathematical model was established to analyze uncertainty sources,quantify the uncertainty components, calculate synthetic uncertainty and extend uncertainty in terms of the dilution of standard solution, the weighing of the samples, the final volume of the samples and the repeatability of instrumental measurement. The experimental results showed that the combined uncertainty of aflatoxin B1 determination in polygala7 tenuifolia- 收稿日期:2022-10-17 基金项目:河北省重点研发计划项目(21344801D) 第一作者:马力辉(1967—),男,河北定州人,河北大学教授,博士,主要从事检测技术及产品创新研究.E-mail:malihui@aliyun.com 通信作者:赵志磊(1977—),男,河北辛集人,河北大学教授,博士,主要从事食品药品质量检测技术.E-mail:15930721279@163.com第5期马力辉等:HPLC法测定远志中黄曲霉毒素B1含量的不确定度评定willd was 0.016 39 μg/kg and the extended uncertainty was 0.032 78 μg/kg.The uncertainty assessment results showed that determination of aflatoxin B1 in polygala tenuifolia willd by HPLC method were reliable, and the uncertainties of measurement in the determination of aflatoxin B1 in polygala tenuifolia willd were mainly come from the repeatability, the fitting of the standard curve, and the use of HPLC. Reference | Related Articles | Metrics | Comments（0）

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Profit distribution of water saving management contract in the fixed investment and return model based on Stackelberg game WANG Xiaosheng,NIU Shuyue Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 453-462.   DOI: 10.3969/j.issn.1000-1565.2023.05.002 Abstract （110）      PDF（pc） （527KB）（55）       Knowledge map    Save Water saving management contract(WSMC)is a kind of water saving investment model by which water costs saved in the future is used to pay for the cost of project renovation and shared with participants. In order to reasonally address the profit distribution problem of WSMC in the fixed investment and return model, this paper takes initial investment amount, contract period and excess water saving income distribution ratio as research objectives, based on Stackebergs master-slave game theory, four profit distribution models under the non-cooperative game between the water users and the water saving service companies are established; and through numerical examples the optimal equilibrium strategies of the four profit distribution models are analyzed. The results show that the water saving service company and the water users respectively achieve the maximum profit value in the case of the Stackelberg equilibrium when they are in the dominant position. For this water saving renovation project, the economic benefit of the project achieves the maximum profit value when the water saving service company is the leader and the initial investment amount is in a priority decision; at the same time, choosing a water saving service company with higher water saving capacity can produce higher water saving benefits.- DOI:10.3969/j.issn.1000-1565.2023.05.002基于Stackelberg博弈的固定投资回报型合同节水管理收益分配王小胜1,牛淑月2(1. 河北工程大学 数理科学与工程学院,河北 邯郸 056038;2.河北工程大学 管理工程与商学院,河北 邯郸 056038)摘 要:合同节水管理(water saving management contract,WSMC)是用节约的水费支付项目改造成本并分享节水收益的一种节水投资模式.为了解决固定投资回报型模式下WSMC的收益分配问题,以初始投资额、合同期以及超额节水收益分配比例为研究对象,基于Stackelberg主从博弈理论,建立了用水户和节水服务公司在非合作博弈下的4种收益分配模型,并通过数值算例对4种模式下的最优策略进行分析.结果表明:节水服务公司和用水户分别在其处于主导地位时的Stackelberg均衡下取得最大利润,对于该节水改造项目而言,在节水服务公司主导且优先决策初始投资额的情况下,项目经济效益较好,同时选择较高节水能力的节水服务公司可以产生更高的节水收益.关键词:合同节水管理;固定投资回报型;收益分配;Stackelberg博弈中图分类号:TV213.4 文献标志码:A 文章编号:1000-1565(2023)05-0453-10Profit distribution of water saving management contract in the fixed investment and return model based on Stackelberg gameWANG Xiaosheng1,NIU Shuyue2(1. School of Mathematics and Physics, Hebei University of Engineering, Handan 056038, China; 2. School of Management Engineering and Business, Hebei University of Engineering, Handan 056038, China)Abstract: Water saving management contract(WSMC)is a kind of water saving investment model by which water costs saved in the future is used to pay for the cost of project renovation and shared with participants. In order to reasonally address the profit distribution problem of WSMC in the fixed investment and return model, this paper takes initial investment amount, contract period and excess water saving income distribution ratio as research objectives, based on Stackebergs master-slave game theory, four profit distribution models under the non-cooperative game between the water users and the water saving service companies are established; and through numerical examples the optimal equilibrium strategies of the four profit distribution models are analyzed. The results show that the water saving service company and the water users respectively achieve the maximum profit value in the case of the Stackelberg equilibrium when they are in the dominant position. For this water saving renovation project, the economic benefit of the project achieves the maximum profit value when the water saving service company is the leader and the initial investment amount is in a priority decision; at the same time, choosing a water saving service company with higher water saving capacity can produce higher water saving benefits.- 收稿日期:2022-08-16 基金项目:国家自然科学基金资助项目(61873084) 第一作者:王小胜(1969—),男,河北邯郸人,河北工程大学教授,博士,主要从事不确定统计预测与决策建模方向研究.E-mail:xswang@hebeu.edu.cn第5期王小胜等:基于Stackelberg博弈的固定投资回报型合同节水管理收益分配 Reference | Related Articles | Metrics | Comments（0）

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Two new record species of Encyrtidae from China(Hymenoptera: Chalcidoidea) WANG Haiyang,ZHANG Chenghui,ZU Guohao Journal of Hebei University(Natural Science Edition)    2023, 43 (4): 420-423.   DOI: 10.3969/j.issn.1000-1565.2023.04.010 Abstract （119）      PDF（pc） （812KB）（54）       Knowledge map    Save Copidosoma coimbatorense Kazmi & Hayat, 1998 and Oobius zahaikevitshi Trjapitzin, 1963 are firstly recorded from Tianjin, China. The morphological characteristics, host and distribution information of adults are described in detail, and the photos of adults are provided. Reference | Related Articles | Metrics | Comments（0）

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Effect of sacubitril valsartan sodium on cardiomyocyte apoptosis by inhibiting Bax/caspase-3 pathway LI Yaqin, LIU Yi,WANG Xiaowei, CUI Honggen,KANG Shixin,XU Zhanwen Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 631-637.   DOI: 10.3969/j.issn.1000-1565.2023.06.009 Abstract （72）      PDF（pc） （1227KB）（54）       Knowledge map    Save To explore the mechanism of sacubitril valsartan sodium(LCZ696)on myocardial apoptosis with heart failure, a heart failure model of SD rats was established by intraperitoneal injection of doxorubicin. After successful model building, rats were randomly divided into HF group, valsartan group, and LCZ696 low(LCZ-L)group, LCZ696 high group(LCZ-H),and control group. After 8 weeks of treatment, the change of MAP, weight and HR were observed. The serum contents of BNP, CK-MB,CK, AST and ALT were detected. The improvement of cardiac function in each group was evaluated by ultrasound. Rat left ventricular myocardial tissue was subjected to HE and Tunel staining; the expression of- DOI:10.3969/j.issn.1000-1565.2023.06.009沙库巴曲缬沙坦钠通过Bax/caspase-3信号通路抑制心肌细胞凋亡李亚芹,刘毅,王潇伟,崔红根,康世鑫,徐占稳(河北大学附属医院 心血管内科,河北 保定 071000)摘 要:为了探索沙库巴曲缬沙坦钠(LCZ696)抑制心衰心肌细胞凋亡的作用机制,腹腔注射盐酸阿霉素构建SD大鼠心衰模型,模型构建成功后随机分为心衰组(HF)、缬沙坦组(Val)、LCZ696低剂量组(LCZ-L)、LCZ696高剂量组(LCZ-H),空白组(Control),治疗8周后观察大鼠平均动脉压(MAP)、体质量、心率(HR)变化,检测血清中脑钠肽(BNP)、肌酸激酶同工酶(CK-MB)、肌酸肌酶(CK)、谷草转氨酶(AST)、谷丙转氨酶(ALT)含量,超声评价各组大鼠心功能改善情况;将大鼠左心室心肌组织进行 HE染色和Tunel染色;检测活化的AKT(P-AKT)、Bax、caspase-3蛋白表达.结果显示:心衰大鼠经Val及LCZ696治疗后超声显示心脏功能均有明显改善,BNP、CK-MB、CK、AST、ALT水平下降,其中LCZ-H组降低最明显;HE染色及Tunel染色显示经治疗后细胞凋亡数减少,心肌组织中P-AKT含量较HF增加,Bax、caspase-3蛋白含量较HF组降低,LCZ-H组减少最显著.综上可知,LCZ696通过抑制Bax/caspase-3信号通路抑制心肌细胞凋亡,改善心功能.关键词:沙库巴曲缬沙坦钠;Bax/caspase-3信号通路;心衰;心肌细胞凋亡 中图分类号:Q952 文献标志码:A 文章编号:1000-1565(2023)06-0631-07Effect of sacubitril valsartan sodium on cardiomyocyte apoptosis by inhibiting Bax/caspase-3 pathwayLI Yaqin, LIU Yi,WANG Xiaowei, CUI Honggen,KANG Shixin,XU Zhanwen(Department of Cardiovascular Medicine, Affiliated Hospital of Hebei University, Baoding 071002,China)Abstract: To explore the mechanism of sacubitril valsartan sodium(LCZ696)on myocardial apoptosis with heart failure, a heart failure model of SD rats was established by intraperitoneal injection of doxorubicin. After successful model building, rats were randomly divided into HF group, valsartan group, and LCZ696 low(LCZ-L)group, LCZ696 high group(LCZ-H),and control group. After 8 weeks of treatment, the change of MAP, weight and HR were observed. The serum contents of BNP, CK-MB,CK, AST and ALT were detected. The improvement of cardiac function in each group was evaluated by ultrasound. Rat left ventricular myocardial tissue was subjected to HE and Tunel staining; the expression of- 收稿日期:2023-06-19 基金项目:河北省卫健委医学科学研究项目(20200203) 第一作者:李亚芹(1973—),女,河北定州人,河北大学附属医院副教授/副主任医师,主要从事冠心病、心力衰竭疾病研究.E-mail:liyqin2014@163.com 通信作者:刘毅(1988—),男,河北安国人,河北大学附属医院主治医师,主要从事冠心病、心衰诊治及介入治疗研究.E-mail:824516368@qq.com王潇伟(1988—),女,河北保定人,河北大学附属医院主治医师,主要从事冠心病及心衰治疗研究.E-mail:775723357@qq.com第6期李亚芹等:沙库巴曲缬沙坦钠通过Bax/caspase-3信号通路抑制心肌细胞凋亡P-AKT, Bax, and caspase-3 proteins was detected. The results showed that heart function improvement after valsartan and LCZ696 treatment.The BNP, CK-MB, CK, AST, and ALT levels were decreased. The most significant reduction was observed in the LCZ-H group. HE and Tunel staining showed decreased myocardial collagen content and apoptosis number after treatment. P-AKT in myocardial tissue increased compared with HF group, Bax and caspase-3 protein decreased compared with HF group, and LCZ-H decreased most significantly. The study show that LCZ696 inhibited cardiomyocyte apoptosis and improved cardiac function by inhibiting the Bax/caspase-3 signaling pathway. Reference | Related Articles | Metrics | Comments（0）

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An improved KCF mixed with deep feature for pedestrian tracking CHEN Xiangyang, ZHOU Yang, YANG Wenzhu Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 665-672.   DOI: 10.3969/j.issn.1000-1565.2023.06.013 Abstract （68）      PDF（pc） （1055KB）（53）       Knowledge map    Save The KCF(kernel correlation filter)algorithm is currently one of the best tracking algorithms, but it has no scale adaptability and cannot solve the occlusion problem. To solve the problems of the KCF algorithm, an improved KCF model, named IKCFMDF(improved KCF mixed with deep feature), is proposed. This model obtains the prediction frame of the target pedestrian by the KCF algorithm, and then compares it with all the pedestrian detection frames obtained through target detection method, and uses the detection frame with the largest intersection-over-union as the new training target, thus achieving the scale adaptation of KCF. By introducing the deep features instead of the HOG features of KCF,the algorithm can avoid the defects that HOG(histogram of oriented gradient)features are insensitive to pose changes and color information of pedestrians. The deep feature is retained when the tracking target is lost, and then the deep feature is compared with the pedestrians in the subsequent videos. If the similarity is greater than the set threshold, it can determine that the target reappears, thereby the occlusion problem of KCF is solved. Compared with the original KCF, the success rate of pedestrian tracking in complex situations is increased by 36%, and compared with the popular neural network models, the proposed model also achieves improvement.- DOI:10.3969/j.issn.1000-1565.2023.06.013融合深度特征的改进KCF行人追踪算法陈向阳,周扬,杨文柱(河北大学 网络空间安全与计算机学院,河北机器视觉工程研究中心,河北 保定 071000)摘 要:KCF(kernel correlation filter)算法是目前追踪效果最好的算法之一,但KCF算法无尺度自适应能力且不能有效解决遮挡问题.针对KCF算法的缺陷,设计了1种改进的KCF模型,IKCFMDF(improved KCF mixed with deep feature).该模型先由KCF算法得到目标行人的预测框,然后与通过目标检测得到的所有行人检测框相比较,将交并比最大的检测框作为新的训练目标,从而实现KCF的尺度自适应;引入深度特征代替原KCF使用的HOG(histogram of oriented gradient)特征,以避免HOG特征对行人的姿势改变和颜色信息不敏感的缺陷;在追踪目标丢失时保留其深度特征,与之后视频中行人的深度特征进行对比,如果相似度大于设定阈值,则可判定目标重新出现,从而有效解决KCF的遮挡问题.与原始KCF相比,改进的模型在复杂情况下,对行人追踪的成功率提升了36%,相比主流的神经网络模型也有少量的提升.关键词:行人追踪;改进的KCF模型;深度特征;目标检测中图分类号:TP391 文献标志码:A 文章编号:1000-1565(2023)06-0665-08An improved KCF mixed with deep feature for pedestrian trackingCHEN Xiangyang, ZHOU Yang, YANG Wenzhu(Hebei Machine Vision Engineering Research Center, School of Cyber Security and Computer, Hebei University, Baoding 071000, China)Abstract: The KCF(kernel correlation filter)algorithm is currently one of the best tracking algorithms, but it has no scale adaptability and cannot solve the occlusion problem. To solve the problems of the KCF algorithm, an improved KCF model, named IKCFMDF(improved KCF mixed with deep feature), is proposed. This model obtains the prediction frame of the target pedestrian by the KCF algorithm, and then compares it with all the pedestrian detection frames obtained through target detection method, and uses the detection frame with the largest intersection-over-union as the new training target, thus achieving the scale adaptation of KCF. By introducing the deep features instead of the HOG features of KCF,the algorithm can avoid the defects that HOG(histogram of oriented gradient)features are insensitive to pose changes and color information of pedestrians. The deep feature is retained when the tracking target is lost, and then the deep feature is compared with the pedestrians in the subsequent videos. If the similarity is greater than the set threshold, it can determine that the target reappears, thereby the occlusion problem of KCF is solved. Compared with the original KCF, the success rate of pedestrian tracking in complex situations is increased by 36%, and compared with the popular neural network models, the proposed model also achieves improvement.- 收稿日期:2022-03-25 基金项目:河北省自然科学基金资助项目(F2018201060) 第一作者:陈向阳(1977—),女,河南三门峡人,河北大学讲师,主要从事机器视觉方向研究.E-mail:chenxyhbu@163.com 通信作者:杨文柱(1968—),男,河北保定人,河北大学教授,博士,主要从事机器视觉方向研究.E-mail:wenzhuyang@163.com第6期陈向阳等:融合深度特征的改进KCF行人追踪算法 Reference | Related Articles | Metrics | Comments（0）

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Evaluation methods of luminous-flux-attenuation activation energy for LED bulb lamp WU Deqi,WU Yiping,CHU Xiaoxing Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 589-594.   DOI: 10.3969/j.issn.1000-1565.2023.06.004LED Abstract （81）      PDF（pc） （498KB）（52）       Knowledge map    Save Under the rated power, temperature stress accelerated life test of BPZ 220/5 W JA55 bulb lamp was performed under thermal stress of 40 ℃ and 60 ℃ respectively, and the failure mechanism of the LED bulb samples was analyzed. A method to evaluate luminous-flux-attenuation activation energy value for LED bulb lamps was proposed, and a theoretical model to calculate luminous-flux-attenuation activation energy value was established in this paper. Thereby, the luminous-flux attenuation activation energy value of 0.67 eV was determined based on the above mentioned theory. Reference | Related Articles | Metrics | Comments（0）

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Coverage optimization for WSNs based on uncovered vertices YIN Boran,MA Jun,CHEN Bohang,WANG Hongchen Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 553-560.   DOI: 10.3969/j.issn.1000-1565.2023.05.015 Abstract （58）      PDF（pc） （1663KB）（51）       Knowledge map    Save In order to further improve the coverage of wireless sensor networks(WSNs)and reduce the number of mobile deployment iterations, this work improves the virtual force between sensors and the virtual force algorithm of Tyson polygon vertices. In view of the discontinuity of the mathematical model of the existing virtual force algorithm, Hookes Law is used heve as the model, so that the virtual force on the sensor is linearly related to the distance. When the distance between sensors is greater than 2RS, virtual force is applied to the nearest sensor node by using the uncovered vertices of Tyson polygons. At the same time, this algorithm lets the step size of the virtual force determine the movement step size of the sensor. The simulation results show that this algorithm can increase the coverage from 93.5% to 99.7%, and reduce the number of iterations to less than 25%. The algorithm in this paper plays an active role in the coverage research of homogeneous wireless sensor networks. Reference | Related Articles | Metrics | Comments（0）

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In vivo and in vitro anti-tumor and immunomodulation effect of compound Taisui oral liquid SHI Bingye,XIA Zihan,WANG Bei,JIN Wenxun Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 506-511.   DOI: 10.3969/j.issn.1000-1565.2023.05.009 Abstract （130）      PDF（pc） （699KB）（49）       Knowledge map    Save To investigate the anti-tumor and immune function of compound Taisui oral liquid,(CTOL)in vivo and in vitro, the cytotoxic effects of different doses of CTOL on SGC7901 cells and Hela cells were detected in vitro. The inhibitory effect of CTOL in different concentrations on tumor cells proliferation was detected by MTT assay. S180 tumor-bearing mice were divided into six groups randomly, which are, model group, CTX group, low-dose of CTOL group, high-dose of CTOL group, low-dose of CTOL + CTX group and high-dose of CTOL + CTX group. CTX was intraperitoneally injected and different doses of CTOL were given by gavages. After 6 weeks of continuous administration,- DOI:10.3969/j.issn.1000-1565.2023.05.009复方太岁口服液体内外抗肿瘤及免疫调节作用 石炳烨1,2,夏子涵2,王蓓2,靳文勋3(1.河北大学附属医院 超声诊断科,河北 保定 071000;2.河北大学 基础医学院,河北 保定 071000;3.保定脑血管病医院 神经外科,河北 保定 071000)摘 要:为探讨复方太岁口服液(compound Taisui oral liquid, CTOL)体内外抗肿瘤及对免疫功能的调节作用,体外采用MTT法检测不同浓度CTOL对SGC7901和Hela 细胞增殖的抑制作用,体内制备S180 荷瘤小鼠模型,实验分为模型组、CTX组、CTOL低剂量组、CTOL高剂量组、CTOL低剂量+CTX组、CTOL高剂量+CTX组.CTX采用腹腔注射给药,CTOL采用灌胃方式给药,连续给药6周后处死小鼠,称取瘤质量和脾质量,计算抑瘤率和脾指数,ELISA法检测血清IL-2和IFN-γ水平.结果显示:不同浓度CTOL对SGC7901和Hela细胞均有抑制作用,且呈剂量依赖关系.体内各实验组瘤质量均明显低于模型组(P<0.05),其中CTOL高剂量+CTX组的抑瘤率最高为70.90%;CTOL低、高剂量+CTX组的脾指数、IL-2、IFN-γ水平均明显高于CTX组(P<0.05).由此可得:CTOL在体内外均可抑制肿瘤的生长,并可改善CTX导致的脾脏损伤及促进IL-2和IFN-γ的分泌.关键词:复方太岁口服液;抗肿瘤;免疫功能中图分类号:R285.5 文献标志码:A 文章编号:1000-1565(2023)05-0506-06In vivo and in vitro anti-tumor and immunomodulation effect of compound Taisui oral liquid SHI Bingye1,2,XIA Zihan2,WANG Bei2,JIN Wenxun3(1. Department of Ultrasound Diagnosis,Affiliated Hospital of Hebei University,Baoding 071000,China;2. School of Basic Medical Sciences,Hebei University, Baoding 071000,China; 3. Department of Neurosurgery,Baoding Cerebrovascular Disease Hospital,Baoding 071000,China)Abstract: To investigate the anti-tumor and immune function of compound Taisui oral liquid,(CTOL)in vivo and in vitro, the cytotoxic effects of different doses of CTOL on SGC7901 cells and Hela cells were detected in vitro. The inhibitory effect of CTOL in different concentrations on tumor cells proliferation was detected by MTT assay. S180 tumor-bearing mice were divided into six groups randomly, which are, model group, CTX group, low-dose of CTOL group, high-dose of CTOL group, low-dose of CTOL + CTX group and high-dose of CTOL + CTX group. CTX was intraperitoneally injected and different doses of CTOL were given by gavages. After 6 weeks of continuous administration,- 收稿日期:2022-10-25 基金项目:河北省医学科学研究课题计划(20211152);河北大学医学学科培育项目(2020B16); 2022年国家级大学生创新创业训练计划项目(202210075017) 第一作者:石炳烨(1983—),男,河北保定人,河北大学附属医院主治医师,河北大学在读硕士研究生,主要从事免疫药理研究.E-mail:2435231348@qq.com 通信作者:靳文勋(1978—),男,河北曲阳人,保定脑血管病医院副主任医师,主要从事神经肿瘤免疫研究.E-mail:jwx780602@163.com第5期石炳烨等:复方太岁口服液体内外抗肿瘤及免疫调节作用 mice were sacrificed, then tumor tissues and spleens were weighed. The tumor inhibition rate and spleen index were calculated and IL-2 and IFN-γ of serum were measured by ELISA. The proliferation of SGC7901 and Hela cells were inhibited by different concentrations of CTOL, and the cytotoxic effect of CTOL on different tumor cells was dose-dependent. The tumor weight of each experimental group was significantly lower than that of the model group(P<0.05), and the tumor inhibition rate of the high-dose of CTOL + CTX group was the highest(70.90%). The spleen index, IL-2 and IFN-γ levels in low-dose and high-dose of CTOL + CTX groups were significantly higher than those in the CTX group(P<0.05). CTOL can inhibit the growth of tumors in vitro and in vivo, promote the secretion of IL-2 and IFN-γ and has protective effect on the damage to spleen caused by CTX in tumor-bearing mice. Reference | Related Articles | Metrics | Comments（0）

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Role of Mahonia bealei in regulating Ferroptosis by AURKA to intervene in Liver hepatocellular carcinoma LI Jun, YANG Kaiping, LIN Dengmei, ZHU Yi, ZHANG Nannan Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 603-615.   DOI: 10.3969/j.issn.1000-1565.2023.06.006 Abstract （76）      PDF（pc） （3623KB）（48）       Knowledge map    Save Through network pharmacology and bioinformatics, we explored the mechanism of Mahonia bealei regulating ferroptosis to interfere with liver hepatocellular carcinoma(LIHC). LIHC gene chip data set was downloaded from GEO database, ferroptosis markers were downloaded from FerrDb database, components and targets of M.bealei were obtained from TCMSP, SymMap and SwissTargetPrediction database. Molecular docking was performed using DS BIOVIA Discovery Studio 2016 v16.1 software to search for hub genes; Using Cytoscape software and STRING platform, PPI analysis was performed on the intersection targets of drug-disease-ferroptosis. The GEPIA and TIMER database were used for differential- DOI:10.3969/j.issn.1000-1565.2023.06.006阔叶十大功劳作用于AURKA调控铁死亡干预肝癌的作用李军1,杨凯平2,林登梅2,朱燚2,张楠楠1(1.贵州中医药大学 基础医学院,贵州 贵阳 550025;2.贵州中医药大学 药学院,贵州 贵阳 550025)摘 要:探索阔叶十大功劳Mahonia bealei调控铁死亡干预肝癌的机制.从GEO数据库下载肝癌的转录组数据集,从FerrDb数据库下载铁死亡相关基因,使用TCMSP、SymMap、SwissTargetPrediction数据库获取M.bealei的成分与靶点;使用DS BIOVIA Discovery Studio 2016 v16.1软件进行分子对接;运用Cytoscape软件及STRING平台,对药物-疾病-铁死亡交集靶点进行PPI分析;利用GEPIA、TIMER数据库进行差异表达分析;利用UALCAN数据库进行临床相关性分析;利用Kaplan-Meier Plotter数据库进行生存分析;使用THPA数据库进行病理学切片分析;使用Western blot进行体外实验验证;最后,进行转录因子的预测,共筛选出5个M.bealei调控铁死亡干预肝癌的关键基因,通过基因差异表达选取3个核心基因AKR1C3、AKR1C1、AURKA,进一步分析发现AURKA与肝癌生存分析、临床参数、病理学结果均显著相关.Western blot实验证实,与HepG2肝癌细胞组相比,M.bealei含药血清组的AURKA蛋白表达上调(P<0.05).通过以上研究认为M.bealei可通过作用于AURKA调控铁死亡而干预肝癌.关键词:阔叶十大功劳;肝癌;铁死亡;AURKA 中图分类号:R273 文献标志码:A 文章编号:1000-1565(2023)06-0603-13Role of Mahonia bealei in regulating Ferroptosis by AURKA to intervene in Liver hepatocellular carcinoma LI Jun1, YANG Kaiping2, LIN Dengmei2, ZHU Yi2, ZHANG Nannan1(1. School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China; 2. School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China)Abstract: Through network pharmacology and bioinformatics, we explored the mechanism of Mahonia bealei regulating ferroptosis to interfere with liver hepatocellular carcinoma(LIHC). LIHC gene chip data set was downloaded from GEO database, ferroptosis markers were downloaded from FerrDb database, components and targets of M.bealei were obtained from TCMSP, SymMap and SwissTargetPrediction database. Molecular docking was performed using DS BIOVIA Discovery Studio 2016 v16.1 software to search for hub genes; Using Cytoscape software and STRING platform, PPI analysis was performed on the intersection targets of drug-disease-ferroptosis. The GEPIA and TIMER database were used for differential- 收稿日期:2023-04-19 基金项目:贵州中医药大学学术新苗项目(贵科合学术新苗2023-28);贵州省科技计划项目(黔科合基础ZK［2023］一般428);2023年贵州中医药大学研究生教育创新计划项目(YCXKYB2023001) 第一作者:李军(1986—),男,山东菏泽人,贵州中医药大学副教授,主要从事肝癌及糖尿病药物方面研究.E-mail: lijun763@gzy.edu.cn 通信作者:张楠楠(1989—),女,河南焦作人,贵州中医药大学讲师,博士,主要从事肝癌及糖尿病药物研究.E-mail: doczn@sina.com第6期李军等:阔叶十大功劳作用于AURKA调控铁死亡干预肝癌的作用expression analysis. Clinical correlation analysis was performed using UALCAN data. Kaplan-Meier Plotter database was used for survival analysis. Pathology section analysis was performed using the THPA database. We used Western blot to Verify in vitro experiments Finally, transcription factor prediction and cell experiments were performed. A total of 5 hub genes regulating ferroptosis and interfering LIHC were screened out. Three core genes, AKR1C3, AKR1C1 and AURKA, were selected through gene differential expression. Further analysis found that AURKA was significantly correlated with LIHC survival analysis, clinical parameters and pathological results. We also verified that AURKA protein expression was up-regulated in M.bealei containing serum group compared with HepG2 hepatocellular carcinoma group by Western blot assay(P<0.05). Through the above research, we believe that the M.bealei can intervene in LIHC by acting on AURKA to regulate ferroptosis. Reference | Related Articles | Metrics | Comments（0）

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A movie recommendation method based on the positive and negative preferences of users with emotional factors ZHANG Bin, DONG Yaqian, XU Jianmin Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 653-664.   DOI: 10.3969/j.issn.1000-1565.2023.06.012 Abstract （65）      PDF（pc） （1041KB）（43）       Knowledge map    Save In the existing movie recommendation methods based on users positive and negative preferences. The users sentiment information was not fully considered, the users positive and negative preferences was not accurate enough, and the recommendation effect was not ideal. In this paper, a method is proposed to improve users positive and negative preferences by using users emotions contained in reviews, and the movie recommendation is realized based on the new preferences. Firstly, the sentiment dictionary in the field of movie was constructed, which is used to realize the mining and quantification of the review data and obtain the sentiment score of user reviews. Secondly, the users preference degree for movies was calculated in order to generate the users positive and negative preference movie sets by considering the users rating and the comment sentiment score comprehensively, then according to the two sets, the users positive and negative preference feature vectors were constructed, and the users positive and negative preferences containing user sentiment were mined. Finally, the similarity, which is between the candidate movie and the users positive and negative preference features, was used to calculate the users final rating for the candidate movie, and the movie recommendation was realized. Compared with traditional - DOI:10.3969/j.issn.1000-1565.2023.06.012基于蕴含情感要素用户正负偏好的电影推荐方法张彬,董雅倩,徐建民(河北大学 网络空间安全与计算机学院,河北 保定 071000)摘 要:针对现有基于用户正负偏好的电影推荐方法未充分考虑用户情感信息、用户正负偏好表示不够准确以及推荐效果不够理想的问题,提出一种利用评论中蕴含的用户情感改进其正负偏好,基于改进偏好实现电影推荐的方法.首先,构建电影领域情感词典,利用该词典实现对评论数据的挖掘与量化,得到用户评论的情感得分;然后,综合考虑用户的评分和评论情感得分计算用户对电影的喜好度,从而生成用户的正、负向偏好电影集合,并基于这2个集合构建用户的正、负向偏好特征向量,挖掘得到蕴含评论情感的用户正负偏好;最后,利用候选电影与用户正、负向偏好特征的相似度计算用户对候选电影的最终评分,实现电影推荐.基于豆瓣数据集的实验结果表明,本文方法的各项指标相对于传统方法有一定提升,其中F1、MAE和MAPE指标分别提升6.10%、3.32%、11.67%.关键词:电影推荐;正向偏好;负向偏好;评论情感中图分类号:TP391 文献标志码:A 文章编号:1000-1565(2023)06-0653-12A movie recommendation method based on the positive and negative preferences of users with emotional factorsZHANG Bin, DONG Yaqian, XU Jianmin(School of Cyber Security and Computer, Hebei University, Baoding 071000, China)Abstract: In the existing movie recommendation methods based on users positive and negative preferences. The users sentiment information was not fully considered, the users positive and negative preferences was not accurate enough, and the recommendation effect was not ideal. In this paper, a method is proposed to improve users positive and negative preferences by using users emotions contained in reviews, and the movie recommendation is realized based on the new preferences. Firstly, the sentiment dictionary in the field of movie was constructed, which is used to realize the mining and quantification of the review data and obtain the sentiment score of user reviews. Secondly, the users preference degree for movies was calculated in order to generate the users positive and negative preference movie sets by considering the users rating and the comment sentiment score comprehensively, then according to the two sets, the users positive and negative preference feature vectors were constructed, and the users positive and negative preferences containing user sentiment were mined. Finally, the similarity, which is between the candidate movie and the users positive and negative preference features, was used to calculate the users final rating for the candidate movie, and the movie recommendation was realized. Compared with traditional - 收稿日期:2023-02-23 基金项目:河北省社会科学基金资助项目(HB21TQ005) 第一作者:张彬(1980—),男,河北涿州人,河北大学高级实验师,博士,主要从事信息检索方向研究.E-mail:zb@hbu.edu.cn 通信作者:徐建民(1966—),男,河北馆陶人,河北大学教授,博士生导师,主要从事信息检索方向研究.E-mail:hbuxjm@hbu.edu.cn第6期张彬等:基于蕴含情感要素用户正负偏好的电影推荐方法methods, the experimental results based on Douban dataset show that the proposed method has certain improvements over the traditional methods, and the indexes of F1, MAE and MAPE are improved by 6.10%, 3.32% and 11.67%, respectively. Reference | Related Articles | Metrics | Comments（0）

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3D PET/MRI image fusion based on improved structure tensor ZHENG Wei, XIA Xueting, ZHANG Xiaodan, MA Zepeng Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 525-538.   DOI: 10.3969/j.issn.1000-1565.2023.05.012 Abstract （61）      PDF（pc） （1653KB）（40）       Knowledge map    Save The fusion of magnetic resonance imaging(MRI)and positron emission computed tomography(PET)can achieve the prevention and diagnosis of Alzheimers disease(AD). Although the current three-dimensional image fusion algorithm based on structural tensor has good spatial characteristics, it can also represent the structural information of the image. This paper proposes structure tensor based on 3D ISobel and double-objective marine predator algorithm(IST), which improves the reliability and stability of the decomposition and makes the detailed information fully expressed. In this paper, the edge region fusion rule of Joint Weight Sum of 3D Energy And Modified Laplacian And Trace Phase Consistency(ETPC)is proposed, which fully describes the local region feature distribution of the fused - DOI:10.3969/j.issn.1000-1565.2023.05.012基于改进结构张量的三维PET/MRI图像融合郑伟1,夏雪婷1,张晓丹2,马泽鹏3(1.河北大学 电子信息工程学院,河北 保定 071002;2.河北大学附属医院 超声诊断科,河北 保定 071000;3.河北大学附属医院 放射科,河北 保定 071000)摘 要:将核磁共振成像(magnetic resonance imaging, MRI)与正电子发射断层成像(positron emission computed tomography, PET)进行融合,可以实现阿尔茨海默病(Alzheimers disease, AD)的预防与诊断.提出了基于3D ISobel与双目标海洋捕食者的结构张量(ISobel and double-objective marine predator algorithm based on structure tensor, IST)分解算法,提高了分解的可靠性与稳定性,使细节信息充分表达.提出了联合三维拉普拉斯加权能量的迹值相位一致性(joint weight sum of 3d energy and modified laplacian and trace phase congruency, ETPC)的边缘区融合规则,充分描述了融合图像局部区域特征分布.融合结果表明,基于改进结构张量的三维PET/MRI图像融合算法解决了结构张量分解欠缺可靠性、融合图像细节信息不充分、局部特征描述不完善的问题,在主观评价和客观评价方面都要优于其他的空间域和变换域的融合方法.关键词:图像融合;结构张量;海洋捕食者算法;三维PET/MRI;相位一致性中图分类号:TP391.9 文献标志码:A 文章编号:1000-1565(2023)05-0525-143D PET/MRI image fusion based on improved structure tensorZHENG Wei1, XIA Xueting1, ZHANG Xiaodan2, MA Zepeng3(1.College of Electronic Information Engineering, Hebei University, Baoding 071002, China;2. Department of Ultrasound Diagnosis, Affiliated Hospital of Hebei University, Baoding 071000, China;3. Department of Radiology, Affiliated Hospital of Hebei University, Baoding 071000, China)Abstract: The fusion of magnetic resonance imaging(MRI)and positron emission computed tomography(PET)can achieve the prevention and diagnosis of Alzheimers disease(AD). Although the current three-dimensional image fusion algorithm based on structural tensor has good spatial characteristics, it can also represent the structural information of the image. This paper proposes structure tensor based on 3D ISobel and double-objective marine predator algorithm(IST), which improves the reliability and stability of the decomposition and makes the detailed information fully expressed. In this paper, the edge region fusion rule of Joint Weight Sum of 3D Energy And Modified Laplacian And Trace Phase Consistency(ETPC)is proposed, which fully describes the local region feature distribution of the fused - 收稿日期:2023-01-06 基金项目:河北省自然科学基金资助项目( F2020201025; H2020201021);河北省高等学校科学技术研究项目(BJ2020030);河北大学附属医院青年科研基金资助项目(2021Q021;2022QB28) 第一作者:郑伟(1972—),女,黑龙江兰西人,河北大学教授,博士,主要从事图像处理与分析、图像安全通信、图像加密和隐藏研究. E-mail:147685650@qq.com 通信作者:马泽鹏(1989—),男,河北保定人,河北大学附属医院放射科主治医师,主要从事脑部和心脏疾病的CT、MR、fMRI等的鉴别诊断研究.E-mail:mzpdan@163.com第5期郑伟等:基于改进结构张量的三维PET/MRI图像融合image. The 3D PET/MRI image fusion algorithm based on improved structural tensor solves the problems of the lack of reliability of structural tensor decomposition, insufficient detail information of fusion image, and imperfect description of local features, and it is better than other fusion methods of spatial domain and transform domain in terms of subjective evaluation and objective evaluation. Reference | Related Articles | Metrics | Comments（0）

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Detection of 2-(trifluoromethyl)phenothiazine in pork by Ic- ELISA LIU Guangxing,HAN Xiaoli,LI Yiqing,WANG Tingxin Journal of Hebei University(Natural Science Edition)    2023, 43 (6): 638-645.   DOI: 10.3969/j.issn.1000-1565.2023.06.010 Abstract （43）      PDF（pc） （1107KB）（37）       Knowledge map    Save In order to quickly analyze the sedative 2-(trifluoromethyl)phenothiazine(TFPTZ)in pork, haptens and complete antigens were synthesized using the azobenzoic acid method and the mixed anhydride method, respectively. Polyclonal antibodies were obtained by immunizing New Zealand white rabbits. Indirect competitive enzyme linked immunosorbent assay(Ic-ELISA)was used to quantitatively analyze TFPTZ in pork. The experimental conditions were as follows: the concentration of the coated antigen was 1.25 μg/mL, and the polyclonal antibody was diluted 16 000 times, Coated at 37 ℃ for 3 h, the blocking solution was 1% BSA of mass fraction, and the TFPTZ diluent is PBS of DMSO with a volume fraction of 10%. The competitive reaction lasted 60 minutes. The correlation coefficient R2 of this method was 0.999 1, and the IC50 was 1.500 2 μg/L, minimum detection limit IC10 is 0.284 1 μg/L, linear range(IC20~IC80)0.430 7~5.225 2 μg/L, the cross reaction rate with five TFPTZ structural analogues is less than 1.2%, the sample addition recovery rate is 93.26%~109.13%, addition recovery rate is 93.26%~109.13%,- DOI:10.3969/j.issn.1000-1565.2023.06.010猪肉中2-(三氟甲基)吩噻嗪的Ic-ELISA检测刘广兴,韩晓利,李昳晴,王庭欣(河北大学 质量技术监督学院,计量仪器与系统国家地方联合工程研究中心,河北省能源计量与安全检测技术重点实验室(筹),河北 保定 071002)摘 要:为了快速检测猪肉中的镇静剂2-(三氟甲基)吩噻嗪(2-(trifluoromethyl)phenothiazine,TFPTZ),分别用偶氮苯甲酸法、混合酸酐法合成了半抗原、完全抗原,通过免疫新西兰大耳白兔获得了多克隆抗体,采用间接竞争酶联免疫方法(Ic-ELISA)对猪肉中TFPTZ进行了定量分析,实验条件:包被抗原浓度1.25 μg/mL,多克隆抗体稀释16 000倍,37 ℃包被3 h,封闭液为质量分数1%的牛血清白蛋白(BSA),TFPTZ稀释液为含体积分数10%的DMSO的PBS,竞争反应60 min.该方法相关系数R2为0.999 1,IC50为1.500 2 μg/L,最低检测限IC10为0.284 1 μg/L,线性(IC20~IC80)为0.430 7~5.225 2 μg/L,与5种TFPTZ结构类似物的交叉反应率小于1.2%,样品添加回收率为93.26%~109.13%,变异系数小于6%,实际样品检测结果与高效液相色谱法测定结果高度一致,表明建立的快速检测猪肉中TFPTZ的Ic-ELISA方法具有良好的准确度,适用于快速检测猪肉中TFPTZ的残留量.关键词:2-(三氟甲基)吩噻嗪;抗原合成;间接竞争酶联免疫方法(Ic-ELISA)中图分类号:Q5-33 文献标志码:A 文章编号:1000-1565(2023)06-0638-08Detection of 2-(trifluoromethyl)phenothiazine in pork by Ic- ELISALIU Guangxing,HAN Xiaoli,LI Yiqing,WANG Tingxin(Hebei Key Laboratory of Energy Metering and Safety Testing Technology, National & Local Joint Engineering Research Center of Metrology Instrument and System, College of Quality and Technical Supervision, Hebei University, Baoding 071002,China)Abstract: In order to quickly analyze the sedative 2-(trifluoromethyl)phenothiazine(TFPTZ)in pork, haptens and complete antigens were synthesized using the azobenzoic acid method and the mixed anhydride method, respectively. Polyclonal antibodies were obtained by immunizing New Zealand white rabbits. Indirect competitive enzyme linked immunosorbent assay(Ic-ELISA)was used to quantitatively analyze TFPTZ in pork. The experimental conditions were as follows: the concentration of the coated antigen was 1.25 μg/mL, and the polyclonal antibody was diluted 16 000 times, Coated at 37 ℃ for 3 h, the blocking solution was 1% BSA of mass fraction, and the TFPTZ diluent is PBS of DMSO with a volume fraction of 10%. The competitive reaction lasted 60 minutes. The correlation coefficient R2 of this method was 0.999 1, and the IC50 was 1.500 2 μg/L, minimum detection limit IC10 is 0.284 1 μg/L, linear range(IC20~IC80)0.430 7~5.225 2 μg/L, the cross reaction rate with five TFPTZ structural analogues is less than 1.2%, the sample addition recovery rate is 93.26%~109.13%, addition recovery rate is 93.26%~109.13%,- 收稿日期:2022-12-18 基金项目:河北省重点研发计划项目(18275502D) 第一作者:刘广兴(1995—),男,河北衡水人,河北大学在读硕士研究生,主要从事食品安全快速检测技术研究.E-mail:603658113@qq.com 通信作者:王庭欣(1965—),女,河北保定人,河北大学教授,主要从事食品安全快速检测技术研究.E-mail:tingxinwang@126.com第6期刘广兴等:猪肉中2-(三氟甲基)吩噻嗪的Ic-ELISA检测and the coefficient of variation is less than 6%. The actual sample detection results are highly consistent with the results determined by high-performance liquid chromatography, indicating that the established Ic-ELISA detection method of TFPTZ has good accuracy and is suitable for rapid detection of TFPTZ residues in pork. Reference | Related Articles | Metrics | Comments（0）

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Supervisor synthesis for Petri nets with unobservable and uncontrollable transitions HAO Jinyuan,GAO Limin,RAN Ning,HUANG Miao,HAO Zhenming Journal of Hebei University(Natural Science Edition)    2023, 43 (5): 517-524.   DOI: 10.3969/j.issn.1000-1565.2023.05.011 Abstract （60）      PDF（pc） （765KB）（32）       Knowledge map    Save A controller synthesis method based on integer linear programming is proposed to solve the forbidden state problem of discrete event systems with both unobservable and uncontrollable events. Firstly, according to the structural characteristics of Petri net, a set of linear constraints for all unobservable transitions and uncontrollable transitions are constructed, and the given constraints were abstracted into integer linear programming problems. Secondly, the allowable linear constraint is obtained by resolving the integer linear programming problem. Finally, the controller is designed based on library invariants and the transformed allowable linear constraints are integrated into Petri net. The simulation- DOI:10.3969/j.issn.1000-1565.2023.05.011含不可观和不可控变迁Petri网的控制器综合方法郝晋渊1,高丽敏2,冉宁2,3,黄淼2,郝真鸣3,4(1.河北大学-中央兰开夏传媒与创意学院,河北 保定 071002;2.河北大学 电子信息工程学院 节能技术研发中心,河北 保定 071002;3.河北大学 电子信息工程学院,河北 保定 071002;4.河北大学 电子信息工程学院 物联网智能技术研究中心,河北 保定 071002)摘 要:针对同时含有不可观和不可控事件的离散事件系统禁止状态问题,提出了一种基于整数线性规划的控制器综合方法.首先,依据Petri网的结构特性构建所有不可观变迁及不可控变迁需满足的线性约束条件集合,将给定约束条件抽象为整数线性规划问题.其次,通过求解整数线性规划问题得到允许线性约束.最后,基于库所不变量的方法设计控制器并将转换后的允许线性约束综合到Petri网中.实验仿真表明提出的方法简单高效,能够保障系统不进入禁止状态,对离散事件系统监控问题具有理论指导意义.关键词:Petri网;禁止状态;约束转换;整数线性规划中图分类号:TP11 文献标志码:A 文章编号:1000-1565(2023)05-0517-08Supervisor synthesis for Petri nets with unobservable and uncontrollable transitionsHAO Jinyuan1,GAO Limin2,RAN Ning2,3,HUANG Miao2,HAO Zhenming3,4(1. HBU-UCLAN School of Media, Communication and Creative Industries, Hebei University, Baoding 071002,China;2. Lab of Energy-Saving Technology, College of Electronic Informational Engineering, Hebei University, Baoding 071002, China; 3. College of Electronic Informational Engineering, Hebei University, Baoding 071002,China; 4. Laboratory of IoT Technology, College of Electronic Informational Engineering, Hebei University, Baoding 071002, China)Abstract: A controller synthesis method based on integer linear programming is proposed to solve the forbidden state problem of discrete event systems with both unobservable and uncontrollable events. Firstly, according to the structural characteristics of Petri net, a set of linear constraints for all unobservable transitions and uncontrollable transitions are constructed, and the given constraints were abstracted into integer linear programming problems. Secondly, the allowable linear constraint is obtained by resolving the integer linear programming problem. Finally, the controller is designed based on library invariants and the transformed allowable linear constraints are integrated into Petri net. The simulation- 收稿日期:2022-03-02 基金项目:国家自然科学基金资助项目(62373132);教育部“春晖计划”合作科研项目(HZKY20220257);中央引导地方科技发展基金资助项目(236Z1602G);河北省高等学校科学技术研究项目(BJ2021008);河北省引进留学人员资助项目(C20190319);河北大学"一省一校"专项基金资助项目(801260201137) 第一作者:郝晋渊(1990—),女,河北保定人,河北大学讲师,主要从事人工智能、信息技术、新媒体传播等研究.E-mail:hjy@hbu.edu.cn 通信作者:冉宁(1978—),男,河北丰宁人,河北大学副教授,主要从事离散事件系统、监控理论等研究.E-mail:ranning87@hotmail.com第5期郝晋渊等:含不可观和不可控变迁Petri网的控制器综合方法results indicate that the suggested technique is uncomplicated and efficient, and can ensure that the system does not enter forbidden state, which has theoretical guiding significance for discrete event system monitoring. Reference | Related Articles | Metrics | Comments（0）